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Product Info Summary
| SKU: | A02536 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Dynamin I DNM1 Antibody
SKU/Catalog Number
A02536
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Dynamin I DNM1 Antibody catalog # A02536. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dynamin I DNM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02536)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Dynamin-1. AA range:740-789
Reactive Species
A02536 is reactive to DNM1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
97.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02536 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of 3T3 cells using Dynamin I Polyclonal Antibody
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Western blot analysis of lysates from mouse brain, using Dynamin-1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Dynamin-1 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Dynamin I DNM1 Antibody (A02536)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Dynamin I DNM1 Antibody
Question
Does A02536 anti-Dynamin I antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
It shows on the product datasheet, A02536 anti-Dynamin I antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-16
Question
My question regards to test anti-Dynamin I antibody A02536 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-Dynamin I antibody A02536 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
The products we sell, including anti-Dynamin I antibody A02536, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-04
Question
Do you have a BSA free version of anti-Dynamin I antibody A02536 available?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Dynamin I antibody A02536 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Dynamin I antibody A02536. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-26
Question
Would anti-Dynamin I antibody A02536 work for WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
According to the expression profile of cervix carcinoma, DNM1 is highly expressed in cervix carcinoma. So, it is likely that anti-Dynamin I antibody A02536 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2019-09-03
Question
We have been able to see staining in mouse platelet. Any tips? Is anti-Dynamin I antibody supposed to stain platelet positively?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
According to literature platelet does express DNM1. According to Uniprot.org, DNM1 is expressed in frontal cortex, brain pns, cervix carcinoma, platelet, among other tissues. Regarding which tissues have DNM1 expression, here are a few articles citing expression in various tissues:
Brain, and PNS, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-08-30
Question
I have a question about product A02536, anti-Dynamin I antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02536 anti-Dynamin I antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-18
Question
We were content with the WB result of your anti-Dynamin I antibody. However we have observed positive staining in platelet cytoplasm. using this antibody. Is that expected? Could you tell me where is DNM1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
From what I have seen in literature, platelet does express DNM1. Generally DNM1 expresses in cytoplasm. Regarding which tissues have DNM1 expression, here are a few articles citing expression in various tissues:
Brain, and PNS, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-07-05
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-Dynamin I antibody A02536. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-30
Question
I see that the anti-Dynamin I antibody A02536 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-05-20
Question
I am interested in using your anti-Dynamin I antibody for g protein-coupled receptor internalization studies. Has this antibody been tested with western blotting on 3t3 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
Thank you for your inquiry. This A02536 anti-Dynamin I antibody is tested on 3t3 cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-11-23
Question
Is a blocking peptide available for product anti-Dynamin I antibody (A02536)?
Verified Customer
Verified customer
Asked: 2018-11-09
Answer
We do provide the blocking peptide for product anti-Dynamin I antibody (A02536). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-11-09
Question
Is this A02536 anti-Dynamin I antibody reactive to the isotypes of DNM1?
Verified Customer
Verified customer
Asked: 2017-06-23
Answer
The immunogen of A02536 anti-Dynamin I antibody is Synthesized peptide derived from human Dynamin I around the non-phosphorylation site of S774. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-23
Question
We are currently using anti-Dynamin I antibody A02536 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
C. Jones
Verified customer
Asked: 2013-08-14
Answer
The anti-Dynamin I antibody (A02536) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-08-14
Question
I was wanting to use your anti-Dynamin I antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma identification?
H. Rodriguez
Verified customer
Asked: 2013-05-17
Answer
It shows on the product datasheet, A02536 anti-Dynamin I antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-05-17
