Overview
| Product Name |
Anti-DYR1A DYRK1A Antibody
See more DYRK1A products |
| Catalog# |
A30487 |
| Pack Size |
100ul |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-DYR1A DYRK1A Antibody catalog # A30487. Tested in IF, WB applications. This antibody reacts with Human, Mouse, Rat. |
| Cite This Product |
Anti-DYR1A DYRK1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30487)
|
| Antibodies Validation |
Antibodies Validation Information
|
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Anti-DYR1A DYRK1A Antibody may replace the following items: sc 28899|sc 130741|sc 100376|sc 12568|sc 12571. |
Product Specs
| Host |
Rabbit |
| Reactive Species |
Human, Mouse, Rat |
| Applications |
IF, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
A peptide derived from human DYR1A. Immunogen sequence location: 21-70 |
Gene/Protein Basic Information For DYRK1A (Source: Uniprot.org, NCBI)
| Uniprot Id | Q13627 |
|---|
| NCBI Gene Id | 1859 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | DYRK1A |
|---|
| Protein Name | Dual specificity tyrosine-phosphorylation-regulated kinase 1A |
|---|
| Superfamily | protein kinase superfamily |
|---|
| Alternative Names | DYRK1A|dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A; DYRK1; DYRK1A; MNBEC 2.7.110EC 2.7.12; MNBH |
|---|
| Molecular Weight | 85584 |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on DYRK1A, check out the DYRK1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for DYRK1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the DYRK1A infographic
Our Boster Quality Guarantee for Anti-DYR1A DYRK1A Antibody covers its use in the following applications.
User need to optimize the dilution ratio for this antibody
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Western blot analysis of lysates from HepG2 cells, using DYR1A Antibody. The lane on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HepG2 cells, using DYR1A Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
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Questions and answers from customers related to A30487 Anti-DYR1A DYRK1A Antibody
17 Related Questions
Question
We are currently using anti-DYR1A antibody A30487 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Asked: 2020-04-17
Answer
The anti-DYR1A antibody (A30487) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-17
Question
Is a blocking peptide available for product anti-DYR1A antibody (A30487)?
Verified Customer
Asked: 2020-02-17
Answer
We do provide the blocking peptide for product anti-DYR1A antibody (A30487). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-17
Question
Please see the WB image, lot number and protocol we used for erythroleukemia using anti-DYR1A antibody A30487. Please let me know if you require anything else.
Verified Customer
Asked: 2020-01-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-21
Question
My team were satisfied with the WB result of your anti-DYR1A antibody. However we have been able to see positive staining in erythroleukemia nucleus using this antibody. Is that expected? Could you tell me where is DYRK1A supposed to be expressed?
Verified Customer
Asked: 2019-10-23
Answer
From literature, erythroleukemia does express DYRK1A. Generally DYRK1A expresses in nucleus. Regarding which tissues have DYRK1A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 8769099, 8975710, 9503011
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Promyelocytic leukemia, Pubmed ID: 9037601
Boster Scientific Support
Answered: 2019-10-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-DYR1A antibody A30487. Let me know if you need anything else.
Verified Customer
Asked: 2019-10-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-17
Question
We ordered your anti-DYR1A antibody for WB on fetal brain in the past. I am using rat, and We want to use the antibody for IF next. you antibody examining fetal brain as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Asked: 2019-08-20
Answer
I have checked the website and datasheets of our anti-DYR1A antibody and it appears that A30487 has been validated on rat in both WB and IF. Thus A30487 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-20
Question
Does A30487 anti-DYR1A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Asked: 2019-07-18
Answer
It shows on the product datasheet, A30487 anti-DYR1A antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-18
Question
Does anti-DYR1A antibody A30487 work on pig WB with erythroleukemia?
Verified Customer
Asked: 2019-05-29
Answer
Our lab technicians have not tested anti-DYR1A antibody A30487 on pig. You can run a BLAST between pig and the immunogen sequence of anti-DYR1A antibody A30487 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-29
Question
Will anti-DYR1A antibody A30487 work for IF with erythroleukemia?
Verified Customer
Asked: 2019-05-21
Answer
According to the expression profile of erythroleukemia, DYRK1A is highly expressed in erythroleukemia. So, it is likely that anti-DYR1A antibody A30487 will work for IF with erythroleukemia.
Boster Scientific Support
Answered: 2019-05-21
Question
We want to test anti-DYR1A antibody A30487 on rat erythroleukemia for research purposes, then I may be interested in using anti-DYR1A antibody A30487 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Asked: 2019-05-14
Answer
The products we sell, including anti-DYR1A antibody A30487, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-14
Question
My question regarding product A30487, anti-DYR1A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Asked: 2018-12-31
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30487 anti-DYR1A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-31
Question
Do you have a BSA free version of anti-DYR1A antibody A30487 available?
Verified Customer
Asked: 2018-06-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DYR1A antibody A30487 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-18
Question
I would like using your anti-DYR1A antibody for positive regulation of protein deacetylation studies. Has this antibody been tested with western blotting on hepg2 cells? We would like to see some validation images before ordering.
Verified Customer
Asked: 2018-04-11
Answer
I appreciate your inquiry. This A30487 anti-DYR1A antibody is validated on hepg2 cells. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-04-11
Question
Is this A30487 anti-DYR1A antibody reactive to the isotypes of DYRK1A?
C. Parker
Asked: 2017-07-26
Answer
The immunogen of A30487 anti-DYR1A antibody is A peptide derived from human DYR1A. Immunogen sequence location: 21-70. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-26
Question
I was wanting to use your anti-DYR1A antibody for IF for rat erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat erythroleukemia identification?
C. Parker
Asked: 2016-05-05
Answer
You can see on the product datasheet, A30487 anti-DYR1A antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-05
Question
We have observed staining in rat forebrain. Are there any suggestions? Is anti-DYR1A antibody supposed to stain forebrain positively?
J. Anderson
Asked: 2015-10-05
Answer
From literature forebrain does express DYRK1A. From Uniprot.org, DYRK1A is expressed in forebrain, fetal brain, promyelocytic leukemia, cervix carcinoma, leukemic t-cell, erythroleukemia, liver, among other tissues. Regarding which tissues have DYRK1A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 8769099, 8975710, 9503011
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Promyelocytic leukemia, Pubmed ID: 9037601
Boster Scientific Support
Answered: 2015-10-05
Question
I see that the anti-DYR1A antibody A30487 works with IF, what is the protocol used to produce the result images on the product page?
R. Mitchell
Asked: 2013-11-14
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-11-14
