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Product Info Summary
| SKU: | A30487 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DYR1A DYRK1A Antibody
SKU/Catalog Number
A30487
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DYR1A DYRK1A Antibody (Catalog# A30487). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DYR1A DYRK1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30487)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human DYR1A. Immunogen sequence location: 21-70
Reactive Species
A30487 is reactive to DYRK1A in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
85.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30487 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of HepG2 cells using Dyrk1A Polyclonal Antibody diluted at 1:500.
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Western Blot analysis of various cells using Dyrk1A Polyclonal Antibody diluted at 1:500.
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Immunofluorescence analysis of HepG2 cells, using DYR1A Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HepG2 cells, using DYR1A Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-DYR1A DYRK1A Antibody (A30487)
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-DYR1A DYRK1A Antibody
Question
We are currently using anti-DYR1A antibody A30487 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
The anti-DYR1A antibody (A30487) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-17
Question
Is a blocking peptide available for product anti-DYR1A antibody (A30487)?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
We do provide the blocking peptide for product anti-DYR1A antibody (A30487). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-17
Question
Please see the WB image, lot number and protocol we used for erythroleukemia using anti-DYR1A antibody A30487. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-21
Question
My team were satisfied with the WB result of your anti-DYR1A antibody. However we have been able to see positive staining in erythroleukemia nucleus using this antibody. Is that expected? Could you tell me where is DYRK1A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
From literature, erythroleukemia does express DYRK1A. Generally DYRK1A expresses in nucleus. Regarding which tissues have DYRK1A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 8769099, 8975710, 9503011
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Promyelocytic leukemia, Pubmed ID: 9037601
Boster Scientific Support
Answered: 2019-10-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-DYR1A antibody A30487. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-17
Question
We ordered your anti-DYR1A antibody for WB on fetal brain in the past. I am using rat, and We want to use the antibody for IF next. you antibody examining fetal brain as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
I have checked the website and datasheets of our anti-DYR1A antibody and it appears that A30487 has been validated on rat in both WB and IF. Thus A30487 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-20
Question
Does A30487 anti-DYR1A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
It shows on the product datasheet, A30487 anti-DYR1A antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-18
Question
Does anti-DYR1A antibody A30487 work on pig WB with erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
Our lab technicians have not tested anti-DYR1A antibody A30487 on pig. You can run a BLAST between pig and the immunogen sequence of anti-DYR1A antibody A30487 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-29
Question
Will anti-DYR1A antibody A30487 work for IF with erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
According to the expression profile of erythroleukemia, DYRK1A is highly expressed in erythroleukemia. So, it is likely that anti-DYR1A antibody A30487 will work for IF with erythroleukemia.
Boster Scientific Support
Answered: 2019-05-21
Question
We want to test anti-DYR1A antibody A30487 on rat erythroleukemia for research purposes, then I may be interested in using anti-DYR1A antibody A30487 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
The products we sell, including anti-DYR1A antibody A30487, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-14
Question
My question regarding product A30487, anti-DYR1A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-12-31
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30487 anti-DYR1A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-12-31
Question
Do you have a BSA free version of anti-DYR1A antibody A30487 available?
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DYR1A antibody A30487 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-18
Question
I would like using your anti-DYR1A antibody for positive regulation of protein deacetylation studies. Has this antibody been tested with western blotting on hepg2 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-04-11
Answer
I appreciate your inquiry. This A30487 anti-DYR1A antibody is validated on hepg2 cells. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-04-11
Question
Is this A30487 anti-DYR1A antibody reactive to the isotypes of DYRK1A?
C. Parker
Verified customer
Asked: 2017-07-26
Answer
The immunogen of A30487 anti-DYR1A antibody is A peptide derived from human DYR1A. Immunogen sequence location: 21-70. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-26
Question
I was wanting to use your anti-DYR1A antibody for IF for rat erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat erythroleukemia identification?
C. Parker
Verified customer
Asked: 2016-05-05
Answer
You can see on the product datasheet, A30487 anti-DYR1A antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-05
Question
We have observed staining in rat forebrain. Are there any suggestions? Is anti-DYR1A antibody supposed to stain forebrain positively?
J. Anderson
Verified customer
Asked: 2015-10-05
Answer
From literature forebrain does express DYRK1A. From Uniprot.org, DYRK1A is expressed in forebrain, fetal brain, promyelocytic leukemia, cervix carcinoma, leukemic t-cell, erythroleukemia, liver, among other tissues. Regarding which tissues have DYRK1A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 8769099, 8975710, 9503011
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Promyelocytic leukemia, Pubmed ID: 9037601
Boster Scientific Support
Answered: 2015-10-05
Question
I see that the anti-DYR1A antibody A30487 works with IF, what is the protocol used to produce the result images on the product page?
R. Mitchell
Verified customer
Asked: 2013-11-14
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-11-14
