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- Table of Contents
Cdh1 antibody (A00063-3) performs well in ELISA, Flow Cytometry, IF, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Flow Cytometry analysis of NIH/3T3 cells using anti-E-Cadherin/Cdh1 antibody (A00063-3).
Overlay histogram showing NIH/3T3 cells stained with A00063-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-E-Cadherin/Cdh1 Antibody (A00063-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing NIH/3T3 cells stained with A00063-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-E-Cadherin/Cdh1 Antibody (A00063-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-E-cadherin/Cdh1 Antibody Picoband®
Cdh1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, WB |
Tested Applications
Cdh1 antibody was positively detected by WB in:
mouse testis tissue, mouse stomach tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Cdh1 antibody was positively detected by IHC in:
mouse colon tissue, rat colon tissue, rat stomach tissue, rat stomach tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Cdh1 antibody was positively detected by IF in:
mouse stomach tissue, rat colon tissue, rat stomach tissue
Cdh1 antibody was positively detected by FC in:
NIH/3T3 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse, Rat
Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg /1x106 cells
Mouse
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Mouse
Tested
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Product Information
Product Name
Anti-E-cadherin/Cdh1 Antibody Picoband®
View all CDH1 Antibodies
View all CDH1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived mouse E-cadherin/Cdh1 recombinant protein (Position: Q23-Q708). Mouse Cdh1 shares 77.9% and 90.7% amino acid (aa) sequence identity with human and rat Cdh1, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
120-130 kDa
Calculated Molecular Weight
98.3 kDa
Background of Cdh1
CDH1 (Cadherin 1), also known as ECAD or UVO, is a protein that in humans is encoded by the CDH1 gene. Cadherin-1 is a classical member of the cadherin superfamily. By Southern analysis of DNA from a panel of mouse-human somatic cell hybrids, Mansouri et al. (1987, 1988) assigned the UVO gene to 16q (16p11-qter). Frebourg et al. (2006) found that in human embryos CDH1 is highly expressed at 4 and 5 weeks in the frontonasal prominence and at 6 weeks in the lateral and medial nasal prominences, and is therefore expressed during critical stages of lip and palate development. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Cite This Product
Anti-E-cadherin/Cdh1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00063-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
91%
High Homology
Rabbit
79%
Moderate Homology
Human
78%
Moderate Homology
Dog
77%
Moderate Homology
Monkey
77%
Moderate Homology
Sheep
76%
Moderate Homology
Cow
76%
Moderate Homology
Zebrafish
46%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Cdh1 antibody
Immunohistochemistry (IHC) protocol for Cdh1 antibody
Immunofluorescence (IF) protocol for Cdh1 antibody
Flow Cytometry (FC) protocol for Cdh1 antibody
Publications
Application:
Species:
Ultrasound-responsive nanodroplets activate the Hippo pathway via ROS-MST1-YAP cascade for breast cancer therapy
Published: 2026-07-04
Journal: ULTRASONICS SONOCHEMISTRY
Application: WB
Impact Factor: 10.20
Species: Mouse
PMID: 42407381
Gut microbiota dysbiosis impairs TGF-β/Smad4 signaling to drive postoperative metastasis in colorectal cancer
Published: 2025-12-15
Journal: Frontiers in Microbiology
Application: IHC
Impact Factor: 4.50
Species: Mouse
PMID: 41472816
Lipoic acid-modified epirubicin liposomal system for tumor-targeted drug delivery and cardiotoxicity reduction
Published: 2025-08-05
Journal: Biomaterials Advances
Application: WB
Impact Factor: 6.00
Species: Mouse
PMID: 40774054
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Product Promise
We promise Anti-E-cadherin/Cdh1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00063-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026