Click image to see more details
Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
Customers Who Bought This Also Bought
Anti-ECE1 Antibody Picoband™
Boster Bio Anti-ECE1 Antibody Picoband™ catalog # A02519-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ECE1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02519-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human ECE1 recombinant protein (Position: M18-T233). Human ECE1 shares 94% and 93.5% amino acid (aa) sequence identity with mouse and rat ECE1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A02519-1 is reactive to ECE1 in Human, Mouse, Rat
A02519-1 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
Submit A Review
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ECE1 using anti-ECE1 antibody (A02519-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse heart tissue lysates,
Lane 3: mouse testis tissue lysates,
Lane 4: HELA whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECE1 antigen affinity purified polyclonal antibody (Catalog # A02519-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECE1 at approximately 87KD. The expected band size for ECE1 is at 87KD.
Gene/Protein Information For ECE1 (Source: Uniprot.Org, NCBI)
Endothelin-converting enzyme 1
peptidase M13 family
EC 3.4.24; EC 126.96.36.199; ECE; ECE1; ECE-1; endothelin converting enzyme 1; endothelin-converting enzyme 1 ECE1 ECE endothelin converting enzyme 1 endothelin-converting enzyme 1|ECE-1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ECE1, check out the ECE1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ECE1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A02519-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu B, Zhu Y, Zhou J, Wei Y, Long C, Chen M, Ling Y, Ge J, Zhuo Y. Cell Stress Chaperones. 2014 Nov;19(6):827-35. Doi: 10.1007/S12192-014-0506-7. Epub 2014 Mar 19. Endoplasmic Reticulum Stress Promotes Amyloid-Beta Peptides Production In Rgc-5 Cells.
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used Anti-ECE1 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-ECE1 Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question