Click image to see more details
Product Info Summary
| SKU: | PA2302 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Mucin-1 MUC1 Antibody Picoband®
SKU/Catalog Number
PA2302
BA3291-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mucin-1 MUC1 Antibody catalog # PA2302. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mucin-1 MUC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2302)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MUC1.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2302 is reactive to MUC1 in Human
Observed Molecular Weight
170 kDa
Calculated molecular weight
122.1 kDa
Background of MUC1
Mucin 1, cell surface associated (MUC1) or polymorphic epithelial mucin (PEM) is a mucin encoded by the MUC1 gene in humans. This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. It is mapped to 1q22. Mucin 1 is a transmembrane mucin normally expressed on the apical borders of secretory epithelial cells. Overexpression of Mucin 1 is often associated with colon, breast, ovarian, lung and pancreatic cancers. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Mucin 1 stimulated ESR1-mediated transcription and contributed to estradiol-mediated growth and survival of breast cancer cells. This gene also can suppress pulmonary innate immunity, and its antiinflammatory activity may play an important modulatory role during microbial infection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2302 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: COLO320 Whole Cell, SW620 Whole Cell, HEPG2 Whole Cell, MCF-7 Whole Cell, JURKAT Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-MUC1 antibody, PA2302, All Western blotting
All lanes: Anti-MUCIN1(PA2302) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: HEPG2 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 122KD
Observed bind size: 170KD
Specific Publications For Anti-Mucin-1 MUC1 Antibody Picoband® (PA2302)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Mucin-1 MUC1 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Mucin-1 MUC1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-Mucin-1 MUC1 Antibody Picoband®
Question
We need using your anti-MUC1 antibody for signal transduction by p53 class mediator resulting in transcription of p21 class mediator studies. Has this antibody been tested with western blotting on hepg2 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-03-11
Answer
We appreciate your inquiry. This PA2302 anti-MUC1 antibody is validated on colo320 whole cell lysate, sw620 whole cell lysate, hepg2 whole cell lysate, jurkat whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-03-11
