Anti-EGFR (phospho-S1046) Antibody

Product Name

Anti-EGFR (phospho-S1046) Antibody

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SKU/Catalog Number

A00023S1046

Size

100ul

Form

Liquid

Description

Boster Bio Anti-EGFR (phospho-S1046) Antibody catalog # A00023S1046. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EGFR (phospho-S1046) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00023S1046)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Reactive Species

A00023S1046 is reactive to EGFR in Human

Applications

A00023S1046 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB: 1:500~1:1000 IHC: 1:50~1:200

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-EGFR (phospho-S1046) Antibody A00023S1046

Western blot (WB) analysis of EGFR (phospho-S1046) polyclonal antibody at 1:500 dilution
Lane1:The Lung tissue lysate of Mouse(40ug)
Lane2:The Lung tissue lysate of Rat(40ug)
Lane3:A549 whole cell lysate(40ug)
Lane4:SKOVCAR3 whole cell lysate(20ug)
Lane5:HCC827 whole cell lysate(10ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

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Figure 2. Immunohistochemistry validation of EGFR using Anti-EGFR (phospho-S1046) Antibody (A00023S1046).

Immunohistochemistry (IHC) analyzes of p-EGFR (S1046) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cell membrane and nucleus staining. Negative control (the right)Using PBS instead of primary antibody

Gene/Protein Information For EGFR (Source: Uniprot.Org, NCBI)

Alternative Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 EGFR ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA epidermal growth factor receptor epidermal growth factor receptor|avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor tyrosine kinase domain|erb-b2 receptor tyrosine kinase 1|proto-oncogene c-ErbB-1|receptor tyrosine-protein kinase erbB-1

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