Anti-RAPGEF2 Antibody Picoband™

Product Name

Anti-RAPGEF2 Antibody Picoband™

View all RAPGEF2 Antibodies

SKU/Catalog Number

A04621-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RAPGEF2 Antibody Picoband™ catalog # A04621-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RAPGEF2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04621-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human RAPGEF2 recombinant protein (Position: S1266-V1499).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04621-1 is reactive to RAPGEF2 in Human

Applications

A04621-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

200-220 kDa

Calculated molecular weight

167.417kDa

Background of RAPGEF2

Rap guanine nucleotide exchange factor 2 is a protein that in humans is encoded by the RAPGEF2 gene. Members of the RAS subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of RAPGEF2 using anti-RAPGEF2 antibody (A04621-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAPGEF2 antigen affinity purified polyclonal antibody (Catalog # A04621-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAPGEF2 at approximately 200-220KD. The expected band size for RAPGEF2 is at 200-220KD.

Click image to see more details

Figure 2. Flow Cytometry analysis of HELA cells using anti-RAPGEF2 antibody (A04621-1).
Overlay histogram showing HELA cells stained with A04621-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAPGEF2 Antibody (A04621-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RAPGEF2 (Source: Uniprot.org, NCBI)

Alternative Names

CNrasGEF; KIAA0313; Neural RAP guanine nucleotide exchange protein; nRap GEP; NRAPGEP; PDZ domain containing guanine nucleotide exchange factor (GEF) 1; PDZGEF1; PDZ-GEF1PDZ domain-containing guanine nucleotide exchange factor 1; RA(Ras/Rap1A-associating)-GEF; RA-GEFDKFZP586O1422; Rap guanine nucleotide exchange factor (GEF) 2; rap guanine nucleotide exchange factor 2; Rap-GEP RAPGEF2 CNrasGEF, NRAPGEP, PDZ-GEF1, PDZGEF1, RA-GEF, RA-GEF-1, RAGEF, Rap-GEP, nRap GEP Rap guanine nucleotide exchange factor 2 rap guanine nucleotide exchange factor 2|PDZ domain containing guanine nucleotide exchange factor (GEF) 1|PDZ domain-containing guanine nucleotide exchange factor 1|RA(Ras/Rap1A-associating)-GEF|Rap guanine nucleotide exchange factor (GEF) 2|cyclic nucleotide ras GEF|neural RAP guanine nucleotide exchange protein|ras/Rap1-associating GEF-1

For more info on RAPGEF2, check out the RAPGEF2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RAPGEF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the RAPGEF2 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis