This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
ESR1 antibody (M00057-2) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
Immunofluorescent analysis of MCF7 cells, using ER alpha Antibody .
Bundle Save
Anti-ER alpha ESR1 Rabbit Monoclonal Antibody
ESR1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
ESR1 antibody was positively detected by IHC in:
human cervix carcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ESR1 antibody was positively detected by ICC/IF in:
MCF7 cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-1:2000
IHC 1:50-1:100
ICC/IF 1:50-1:100
FC 1:30
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
FC
Human
Recommend
Tested
Recommend
Recommend
Mouse
Recommend
Recommend
Rat
Recommend
Recommend
Product Information
Product Name
Anti-ER alpha ESR1 Rabbit Monoclonal Antibody
View all ESR1 Antibodies
View all ESR1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IE-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ER alpha
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
60 kDa
Calculated Molecular Weight
66.2 kDa
Cite This Product
Anti-ER alpha ESR1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00057-2)
Protocols
Immunohistochemistry (IHC) protocol for ESR1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ESR1 antibody
Publications
Application:
Species:
The heterogeneous subclones might be induced by cycling hypoxia which was aggravated along with the luminal A tumor growth
Published: 2022-05-30
Journal: TISSUE & CELL
Application: WB,IHC,IF
Impact Factor: 2.59
Species: Human
PMID: 35691201
Review
Have you used Anti-ER alpha ESR1 Rabbit Monoclonal Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
1 Review For Anti-ER alpha ESR1 Rabbit Monoclonal Antibody
Western blot using Anti-ER/ESR1 Antibody (M00057-2) in mouse brain tissues showed clear, specific ESR1 bands across normal, disease model, and AB drug-treated groups, with β‑actin as a consistent loading control.
| SKU | M00057-2 |
|---|---|
| Application | Western Blot |
| Sample | mouse brain tissue |
| Sample Processing Description | Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4°C for 2 hours, centrifuged to collect the supernatant, and protein concentration was determined. After adjusting the concentration, samples were mixed with 5× protein loading buffer, denatured by heating at 95–100°C for 10 minutes, and 15 μL of protein was loaded per lane for SDS-PAGE. |
| Other Reagents | 5% non-fat milk |
| Primary Antibody | GAPDH Antibody Picoband® |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | goat anti rabbit secondary antibodies |
| Secondary Incubation | 1:5000, 1 h in RT |
| Detection | Substrate: ECL substrate, Image system:ChemiDoc MP |
| Results Summary | This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use. |
Yetao Ju, Liaoning University of Traditional Chinese Medicine
Verified customer
Submitted 2026-03-05
Product Promise
We promise Anti-ER alpha ESR1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00057-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026