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CD86 antibody (M00220-1) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 49 Publications

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

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Anti-CD86/B7 2 Rabbit Monoclonal Antibody

Name: Anti-CD86/B7 2 Rabbit Monoclonal Antibody
Brand: Boster Bio
SKU: M00220-1
Price: 370 USD
Availability: InStock
Rating: 100 (2 reviews)

CD86 antibody

49 Publications|

2 Reviews

|Submit a Review

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IP, IF, IHC, ICC, WB

Tested Applications

CD86 antibody was positively detected by WB in:

human Daudi whole cell, human Raji whole cell, human HEL whole cell, rat PC-12 whole cell, rat C6 whole cell, mouse RAW264.7 whole cell, mouse A20 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CD86 antibody was positively detected by IHC in:

human tonsil tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ICC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-CD86/B7 2 Rabbit Monoclonal Antibody
View all CD86 Antibodies

Form

Liquid

Uniprot

P42081

Gene ID

942

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

BFF-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CD86

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

38 kDa

Calculated Molecular Weight

37.7 kDa

Cite This Product

Anti-CD86/B7 2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00220-1)

Protocols

Western Blot protocol for CD86 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for CD86 antibody

Review Protocol

Publications

AB4-Loaded Nanomicelle Hydrogel Promotes Targeting of the Dysregulated Diabetic Wound Microenvironment via Coordinated Multistage Repair

Published: 2026-08-14

Journal: Gels

Application: WB

Impact Factor: 6.40

Species: Mouse

PMID: 42644966

SKU	M00220-1
Application	Western Blot
Sample	mouse lung cancer tissue
Sample Processing Description	Tissue samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98°C. Twenty microliters of each protein sample were loaded per lane onto an SDS-PAGE gel.
Other Reagents	5% Non-fat milk
Primary Antibody	CD86/B7 2 Rabbit Monoclonal Antibody
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation	1 hour in room temperature
Detection	Substrate: ECL reagent, Imaging system:Tanon
Results Summary	This antibody is highly specific and efficient, with a clear target band and no nonspecific bands.

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Free Secondary Antibody

Anti-CD86/B7 2 Rabbit Monoclonal Antibody

CD86 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

AB4-Loaded Nanomicelle Hydrogel Promotes Targeting of the Dysregulated Diabetic Wound Microenvironment via Coordinated Multistage Repair

Carrier-Free Nanoparticles Enhance Photothermal–Immune Therapy via Metabolic Reprogramming in Triple-Negative Breast Cancer

CCL17-neutralizing and esterase-responsive core-shell microgels for endogenous Tregs recruitment and functional enhancement in myocardial infarction

Metabolic Reprogramming Nanoplatform for Broad-Spectrum Lactate Suppression to Reverse High Intensity Focused Ultrasound-Induced Immunosuppression in Hepatocellular Carcinoma Therapy

Inhibition of RGS1 ameliorates obesity-associated macrophage dysfunction and metaflammation through restoration of PINK1/Parkin-mediated mitophagy

Pulmonary microbiota-associated formononetin modulates microglial activation in asthma-related anxiety

miR-33a promotes peri-implantitis by facilitating M1-type polarization of macrophages and inhibiting osteogenesis

Dunhuang Daxiefei Decoction ameliorates acute lung injury via the HIF-1α/glycolysis/H3K18la axis

Fraxin Attenuates Rheumatoid Arthritis by Regulating Macrophage Polarization and Inhibiting Fibroblast-like Synoviocyte Proliferation

Human umbilical cord mesenchymal stem cells alleviate hypoxic-ischemia-induced white-matter injury in neonatal rats by regulating polarization of microglia

Platycodin D alleviates idiopathic pulmonary fibrosis by targeting PPP2R1A and regulating the PP2A-mediated PI3K/Akt pathway to inhibit fibroblast transdifferentiation

Ptbp2 Alleviates Neuroinflammation and Blood–brain Barrier Disruption via Modulating Microglial Polarization in Ischemic Stroke

NsPEFs-enriched ADSCs-EVs alleviate osteoarthritis via RSPO3-mediated dual pro-chondrogenic and pro-M2 macrophage properties

Neuroprotective Effects of C-Phycocyanin in Chronic Unpredictable Mild Stress-Exposed Rats: Regulation of Neuroinflammation and CREB/BDNF Signaling

Stress-trained microalgae robots with probiotics backpack and intestinal brake for inflammatory bowel disease management

Geniposidic Acid from Ginger-processed Eucommiae Cortex Alleviates Rheumatoid Arthritis by Modulating Macrophage Lactylation Induced by Exosomes from Inflammatory Fibroblast-Like Synoviocytes

Construction of ROS-responsive cascade-targeted liposomes and investigation of their anti-AD effects

Aqueous extract of Saposhnikovia divaricata root alleviates rheumatoid arthritis by acting on TNF-α and RAGE signaling pathways

Wharton’s jelly-derived mesenchymal stem cells ameliorate high altitude-induced heart injury by promoting type 2 macrophage polarization via COX2-PGE2 pathway

Interleukin-37 modulates microglial phenotype and inhibits inflammatory response via the MyD88/NF-κB pathway in lipopolysaccharide-induced neuroinflammation

FGF mimetic peptide-modified electrospun nanocomposite fibrous membranes for accelerating infectious diabetic wound healing by synergistic antibacterial and pro-angiogenesis effects

Regulation of Nrf2/GPX4 Signaling Pathway by Hyperbaric Oxygen Protects Against Depressive Behavior and Cognitive Impairment in a Spinal Cord Injury Rat Model

Application of sodium alginate and polyethylene glycol bilayer multifunctional hydrogel microneedles in infectious and diabetic wounds

Dual-Functional Micelles for Codelivery of Sorafenib and Dehydrodiisoeugenol in Treatment of Hepatocellular Carcinoma

Modulation of Macrophage Polarization in Rheumatoid Arthritis Therapy via Targeted Delivery of Bulleyaconitine A

Sustained-Release H2S Nanospheres Regulate the Inflammatory Microenvironment of Wounds, Promote Angiogenesis and Collagen Deposition, and Accelerate Diabetic Wound Healing

Follicle-stimulating hormone peptide-conjugated liposomes in the treatment of epithelial ovarian cancer through the induction of M2-to-M1 macrophage repolarization

Identification of m6A methyltransferase-related WTAP and ZC3H13 predicts immune infiltrates in glioblastoma

(+)-Borneol inhibits neuroinflammation and M1 phenotype polarization of microglia in epileptogenesis through the TLR4-NFκB signaling pathway

Deleting fibroblast growth factor 2 in macrophages aggravates septic acute lung injury by increasing M1 polarization and inflammatory cytokine secretion

An injectable self-lubricating supramolecular polymer hydrogel loaded with platelet lysate to boost osteoarthritis treatment

Dendritic cell-based biomimetic nanoparticles for foot-and-mouth disease induce robust cellular immunity

Huanshaodan regulates microglial glucose metabolism reprogramming to alleviate neuroinflammation in AD mice through mTOR/HIF-1α signaling pathway

rTMS applied to the PFC relieves neuropathic pain and modulates neuroinflammation in CCI rats

AMPK Regulates M1 Macrophage Polarization through the JAK2/STAT3 Signaling Pathway to Attenuate Airway Inflammation in Obesity-Related Asthma

Cu+/Ca2+ Dual-Overload Strategy Assisted by Metabolic-Symbiotic-Destruction for Tumor Apoptosis and Tumor Immune Activation

Mannose-modified celastrol liposomes targeted activated macrophages for rheumatoid arthritis treatment in vitro and in vivo evaluation

Effect and mechanism of chlorogenic acid on cognitive dysfunction in mice by lipopolysaccharide-induced neuroinflammation

Oxymatrine blocks the NLRP3 inflammasome pathway, partly downregulating the inflammatory responses of M1 macrophages differentiated from THP-1 monocytes

Reversing inflammatory microenvironment by a single intra-articular injection of multi-stimulus responsive lipogel to relieve rheumatoid arthritis and promote joint repair

The involvement of Th1 cell differentiation in the anti-tumor effect of purified polysaccharide from Sanghuangporus vaninii in colorectal cancer via multi-omics analysis

Human Neural Stem Cell Secretome Inhibits Lipopolysaccharide-Induced Neuroinflammation Through Modulating Microglia Polarization by Activating Peroxisome Proliferator-Activated Receptor Gamma

Wharton's jelly mesenchymal stem cell-derived small extracellular vesicles as natural nanoparticles to attenuate cartilage injury via microRNA regulation

Sanguinarine, identified as a natural alkaloid LSD1 inhibitor, suppresses lung cancer cell growth and migration

FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis

Mitochondrial targeted melanin@mSiO2 yolk-shell nanostructures for NIR-Ⅱ-driven photo-thermal-dynamic/immunotherapy

Growth differentiation factor-15 promotes immune escape of ovarian cancer via targeting CD44 in dendritic cells

CPEB3 inhibits epithelial-mesenchymal transition by disrupting the crosstalk between colorectal cancer cells and tumor-associated macrophages via IL-6R/STAT3 signaling

Network pharmacology identification of mechanisms of cerebral ischemia injury amelioration by Baicalin and Geniposide

Review

Have you used Anti-CD86/B7 2 Rabbit Monoclonal Antibody?

2 Reviews For Anti-CD86/B7 2 Rabbit Monoclonal Antibody

This antibody is highly specific and efficient, with a clear target band and no nonspecific bands.

The antibody is highly efficient and specific, showing a clear target band with no nonspecific bands.

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