This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
ERBB3 antibody (PA1880) performs well in WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
Western blot analysis of ERBB3 using anti-ERBB3 antibody (PA1880).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human MDA-MB453 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human T-47D whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB3 antigen affinity purified polyclonal antibody (PA1880) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ERBB3 at approximately 180 kDa. The expected band size for ERBB3 is at 148 kDa.
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human MDA-MB453 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human T-47D whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB3 antigen affinity purified polyclonal antibody (PA1880) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ERBB3 at approximately 180 kDa. The expected band size for ERBB3 is at 148 kDa.
Bundle Save
Anti-ErbB 3/ERBB3 Antibody Picoband®
ERBB3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | WB |
Tested Applications
ERBB3 antibody was positively detected by WB in:
human MCF-7 whole cell, human MDA-MB453 whole cell, human 293T whole cell, human T-47D whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
Human
Tested
Dog
Predicted
Rabbit
Predicted
Bovine
Predicted
Sheep
Predicted
Monkey
Predicted
Product Information
Product Name
Anti-ErbB 3/ERBB3 Antibody Picoband®
View all ERBB3 Antibodies
View all ERBB3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ErbB 3.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
180 kDa
Calculated Molecular Weight
148.1 kDa
Background of ERBB3
ERBB3 (V-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3) also known as ONCOGENE ERBB3 or HER3, is an enzyme that in humans is encoded by the ERBB3 gene. This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. By in situ hybridization, Kraus et al. (1989) mapped the ERBB3 gene to chromosome 12q13. Carraway et al. (1994) demonstrated that ERBB3 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Most notably, EGFR and ErbB2 became markedly more promiscuous as the threshold was lowered, whereas ErbB3did not. Engelman et al. (2007) found that amplification of MET caused gefitinib resistance by driving ERBB3-dependent activation of phosphoinositide 3-kinase, a pathway though to be specific to EGFR/ERBB family receptors.
Cite This Product
Anti-ErbB 3/ERBB3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1880)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
100%
High Homology
Rabbit
90%
High Homology
Cow
90%
High Homology
Sheep
90%
High Homology
Monkey
90%
High Homology
Mouse
80%
Moderate Homology
Rat
80%
Moderate Homology
Zebrafish
60%
Low homology
Drosophila
25%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ERBB3 antibody
Review
Have you used Anti-ErbB 3/ERBB3 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-ErbB 3/ERBB3 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PA1880
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026