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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Boster Bio Anti-ERK2/MAPK1 Antibody catalog # PA1310-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERK2/MAPK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1310-1)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the C-terminus of human ERK2 (324-335aa IAEAPFKFDMEL), identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PA1310-1 is reactive to MAPK1 in Human, Mouse, Rat
PA1310-1 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
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Anti-ERK2 antibody, PA1310-1, All Western blotting
All lanes: Anti-MAPK1(PA1310-1) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 40ug
Lane 2: Rat Liver Tissue Lysate at 40ug
Lane 3: Rat Brain Tissue Lysate at 40ug
Lane 4: Rat Thymus Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: MM231 Whole Cell Lysate at 40ug
Lane 9: CEM Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
Gene/Protein Information For MAPK1 (Source: Uniprot.Org, NCBI)
Mitogen-activated protein kinase 1
protein kinase superfamily
EC 2.7.11; EC 220.127.116.11; ERK; ERK2; ERK-2; ERK2MAP kinase isoform p42; ERT1; Extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAPK 1; MAPK1; MAPK2; mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p38; p40; p41; p41mapk; p42mapk; p42-MAPK; PRKM1; PRKM1MAPK 2; PRKM2; protein tyrosine kinase ERK2 MAPK1 ERK, ERK-2, ERK2, ERT1, MAPK2, NS13, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK mitogen-activated protein kinase 1 mitogen-activated protein kinase 1|MAP kinase 1|MAP kinase 2|MAPK 2|extracellular signal-regulated kinase 2|mitogen-activated protein kinase 2|protein tyrosine kinase ERK2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MAPK1, check out the MAPK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MAPK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PA1310-1 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Disulfiram inhibits TGF-?-induced epithelial-mesenchymal transition and stem-like features in breast cancer via ERK/NF-?B/Snail pathway
eIF3i activity is critical for endothelial cells in tumor induced angiogenesis through regulating VEGFR and ERK translation
Ma Hr, Wang J, Chen Yf, Chen H, Wang Ws, Aisa Ha. Int J Mol Med. 2014 Jun;33(6):1627-34. Doi: 10.3892/Ijmm.2014.1722. Epub 2014 Apr 3. Icariin And Icaritin Stimulate The Proliferation Of Skbr3 Cells Through The Gper1-Mediated Modulation Of The Egf...
Sang J, Chen Y, Tao Y. Mol Med Rep. 2011 Nov-Dec;4(6):1163-7. Doi: 10.3892/Mmr.2011.535. Epub 2011 Jul 15. Nitric Oxide Inhibits Gastric Cancer Cell Growth Through The Modulation Of The Akt Pathway.
Li Xl, Chen Xq, Zhang Mn, Chen N, Nie L, Xu M, Gong J, Shen Pf, Su Zz, Weng X, Tan Jy, Zhao T, Zeng H, Zhou Q. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3871-81. Ecollection 2015. Sox9 Was Involved In Tkis Resistance In Renal Cell Carcinoma Via Raf/M...
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