Anti-ERK2/MAPK1 Antibody Picoband®

Product Name

Anti-ERK2/MAPK1 Antibody Picoband®

View all MAPK1 Antibodies

SKU/Catalog Number

PA1310-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ERK2/MAPK1 Antibody catalog # PA1310-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ERK2/MAPK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1310-1)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ERK2, identical to the related rat and mouse sequences.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1310-1 is reactive to MAPK1 in Human, Mouse, Rat

Observed Molecular Weight

41 kDa

Calculated molecular weight

41.4 kDa

Background of MAPK1

Mitogen-activated protein kinase 1, also known as MAPK1, p42MAPK, and ERK2, is an enzyme which in humans is encoded by the MAPK1 gene. The gene is mapped to 22q11.2. And The protein encoded by this gene is a member of the MAP kinase family. The deduced 360-amino acid human ERK2 protein shares 98% identity with rat Erk2. The MAP kinase ERK2 is widely involved in eukaryotic signal transduction. Upon activation, it translocates to the nucleus of the stimulated cell, where it phosphorylates nuclear targets. Futhermore, the ERK pathway is necessary for experience-dependent plasticity and for long-term potentiation of synaptic transmission in the developing visual cortex. And the MAPK pathway is also involved in responses to NTN1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1310-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: Human Placenta Tissue, Rat Liver Tissue, Rat Brain Tissue, Rat Thymus Tissue, HELA Whole Cell, SMMC Whole Cell, COLO320 Whole Cell, MM231 Whole Cell, CEM Whole Cell

Validation Images & Assay Conditions

See full target information MAPK1

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