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Product Info Summary
| SKU: | PB9867 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-FCGRT Antibody Picoband®
SKU/Catalog Number
PB9867
PB0911 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FCGRT Antibody Picoband® catalog # PB9867. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FCGRT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9867)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse FCGRT recombinant protein (Position: Q54-D291). Mouse FCGRT shares 70.2% and 92.4% amino acid (aa) sequence identity with human and rat FCGRT, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9867 is reactive to Fcgrt in Mouse
Observed Molecular Weight
50 kDa
Calculated molecular weight
40.1 kDa
Background of Fcgrt
IgG receptor FcRn large subunit p51 is a protein that in humans is encoded by the FCGRT gene. This gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies from mother to fetus across the placenta. This protein also binds immunoglobulin G to protect the antibody from degradation. In addition, it is postulated that long-range cis inactivation of the FCGRT gene is responsible for hypercatabolism of IgG in myotonic dystrophy. The FCGRT gene is closely situated to the DMPK gene, which is mutant in DM.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9867 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: mouse liver tissue
IHC: mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of FCGRT using anti-FCGRT antibody (PB9867).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGRT antigen affinity purified polyclonal antibody (Catalog # PB9867) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FCGRT at approximately 50 kDa. The expected band size for FCGRT is at 40 kDa.
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IHC analysis of FCGRT using anti-FCGRT antibody (PB9867).
FCGRT was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FCGRT Antibody (PB9867) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-FCGRT Antibody Picoband® (PB9867)
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7 Customer Q&As for Anti-FCGRT Antibody Picoband®
Question
Would PB9867 anti-FCGRT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
As indicated on the product datasheet, PB9867 anti-FCGRT antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-21
Question
Does anti-FCGRT antibody PB9867 work for WB with mucosa of transverse colon?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
According to the expression profile of mucosa of transverse colon, FCGRT is highly expressed in mucosa of transverse colon. So, it is likely that anti-FCGRT antibody PB9867 will work for WB with mucosa of transverse colon.
Boster Scientific Support
Answered: 2019-12-25
Question
I was wanting to use your anti-FCGRT antibody for WB for mouse mucosa of transverse colon on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse mucosa of transverse colon identification?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
It shows on the product datasheet, PB9867 anti-FCGRT antibody has been validated for ELISA, IHC, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse mucosa of transverse colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-12
Question
We are currently using anti-FCGRT antibody PB9867 for mouse tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
The anti-FCGRT antibody (PB9867) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-09
Question
I see that the anti-FCGRT antibody PB9867 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-12-03
Question
Will anti-FCGRT antibody PB9867 work on feline WB with placenta?
T. Mitchell
Verified customer
Asked: 2017-04-10
Answer
Our lab technicians have not validated anti-FCGRT antibody PB9867 on feline. You can run a BLAST between feline and the immunogen sequence of anti-FCGRT antibody PB9867 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-10
Question
Is this PB9867 anti-FCGRT antibody reactive to the isotypes of FCGRT?
D. Brown
Verified customer
Asked: 2015-11-30
Answer
The immunogen of PB9867 anti-FCGRT antibody is E. coli-derived mouse FCGRT recombinant protein (Position: Q54-D291). Mouse FCGRT shares 70.2% and 92.4% amino acid (aa) sequence identity with human and rat FCGRT, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-11-30
