Anti-FGFR1 Rabbit Monoclonal Antibody

FGFR1 antibody

Boster Bio Anti-FGFR1 Rabbit Monoclonal Antibody catalog # M00098. Tested in WB, ICC/IF applications. This antibody reacts with Human.

Product Info Summary

SKU: M00098
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IF, ICC, WB

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Product Name

Anti-FGFR1 Rabbit Monoclonal Antibody

View all FGFR1 Antibodies

SKU/Catalog Number

M00098

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-FGFR1 Rabbit Monoclonal Antibody catalog # M00098. Tested in WB, ICC/IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FGFR1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00098)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EHC-6

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human FGFR1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00098 is reactive to FGFR1 in Human

Applications

M00098 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

91.868kDa

Background of FGFR1

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For FGFR1 (Source: Uniprot.org, NCBI)

Gene Name

FGFR1

Full Name

Fibroblast growth factor receptor 1

Weight

91.868kDa

Superfamily

protein kinase superfamily

Alternative Names

basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; CD331 antigen; CD331; CEK; EC 2.7.10; EC 2.7.10.1; FGF R1; FGFBR; FGFR1; FGFR-1; fibroblast growth factor receptor 1; FLGH3; FLJ99988; Flt-2; FLT2H4; Fms-like tyrosine kinase 2; fms-related tyrosine kinase 2; H2; H5; HBGFR; heparin-binding growth factor receptor; hydroxyaryl-protein kinase; KAL 2; KAL2; N-SAM; OGD; Proto-oncogene c-Fgr; soluble FGFR1 variant 1; soluble FGFR1 variant 2 FGFR1 BFGFR, CD331, CEK, ECCL, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1 fibroblast growth factor receptor 1 fibroblast growth factor receptor 1|FGFR1/PLAG1 fusion|FMS-like tyrosine kinase 2|basic fibroblast growth factor receptor 1|fms-related tyrosine kinase 2|heparin-binding growth factor receptor|hydroxyaryl-protein kinase|proto-oncogene c-Fgr

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on FGFR1, check out the FGFR1 Infographic

FGFR1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FGFR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-FGFR1 Rabbit Monoclonal Antibody

Question

I was wanting to use your anti-FGFR1 Rabbit Monoclonal antibody for WB for human pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreas identification?

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

As indicated on the product datasheet, M00098 anti-FGFR1 Rabbit Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-03

Question

My boss were satisfied with the WB result of your anti-FGFR1 Rabbit Monoclonal antibody. However we have seen positive staining in brain colon cell membrane using this antibody. Is that expected? Could you tell me where is FGFR1 supposed to be expressed?

E. Jha

Verified customer

Asked: 2020-02-14

Answer

According to literature, brain colon does express FGFR1. Generally FGFR1 expresses in cell membrane. Regarding which tissues have FGFR1 expression, here are a few articles citing expression in various tissues:
Brain, and Colon, Pubmed ID: 16421571
Foreskin fibroblast, and Umbilical vein, Pubmed ID: 1652059
Liver, Pubmed ID: 1846977
Lung, Pubmed ID: 1650441
Neonatal brain stem, Pubmed ID: 1697263
Pancreas, Testis, and Uterus, Pubmed ID: 15489334
Placenta, Pubmed ID: 2159626, 2162671
Placenta, and Testis, Pubmed ID: 14702039
Plasma, Pubmed ID: 16335952
Teratocarcinoma, Pubmed ID: 1722683

Boster Scientific Support

Answered: 2020-02-14

Question

Is this M00098 anti-FGFR1 Rabbit Monoclonal antibody reactive to the isotypes of FGFR1?

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

The immunogen of M00098 anti-FGFR1 Rabbit Monoclonal antibody is A synthesized peptide derived from human FGFR1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-22

Question

I have a question about product M00098, anti-FGFR1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00098 anti-FGFR1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-09

Question

Is a blocking peptide available for product anti-FGFR1 Rabbit Monoclonal antibody (M00098)?

T. Edwards

Verified customer

Asked: 2019-11-07

Answer

We do provide the blocking peptide for product anti-FGFR1 Rabbit Monoclonal antibody (M00098). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-11-07

Question

I am interested in to test anti-FGFR1 Rabbit Monoclonal antibody M00098 on human pancreas for research purposes, then I may be interested in using anti-FGFR1 Rabbit Monoclonal antibody M00098 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

The products we sell, including anti-FGFR1 Rabbit Monoclonal antibody M00098, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-31

Question

We have been able to see staining in human testis uterus. Do you have any suggestions? Is anti-FGFR1 Rabbit Monoclonal antibody supposed to stain testis uterus positively?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

From literature testis uterus does express FGFR1. From Uniprot.org, FGFR1 is expressed in body of pancreas, placenta, neonatal brain stem, teratocarcinoma, lung, liver, placenta testis, brain colon, pancreas, testis uterus, foreskin fibroblast umbilical vein, plasma, among other tissues. Regarding which tissues have FGFR1 expression, here are a few articles citing expression in various tissues:
Brain, and Colon, Pubmed ID: 16421571
Foreskin fibroblast, and Umbilical vein, Pubmed ID: 1652059
Liver, Pubmed ID: 1846977
Lung, Pubmed ID: 1650441
Neonatal brain stem, Pubmed ID: 1697263
Pancreas, Testis, and Uterus, Pubmed ID: 15489334
Placenta, Pubmed ID: 2159626, 2162671
Placenta, and Testis, Pubmed ID: 14702039
Plasma, Pubmed ID: 16335952
Teratocarcinoma, Pubmed ID: 1722683

Boster Scientific Support

Answered: 2019-10-10

Question

We are currently using anti-FGFR1 Rabbit Monoclonal antibody M00098 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-21

Answer

The anti-FGFR1 Rabbit Monoclonal antibody (M00098) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-21

Question

Do you have a BSA free version of anti-FGFR1 Rabbit Monoclonal antibody M00098 available?

Verified Customer

Verified customer

Asked: 2018-06-22

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FGFR1 Rabbit Monoclonal antibody M00098 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-06-22

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-FGFR1 Rabbit Monoclonal antibody M00098. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-01-25

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-25

Question

Will anti-FGFR1 Rabbit Monoclonal antibody M00098 work on monkey IF with plasma?

Verified Customer

Verified customer

Asked: 2017-12-18

Answer

Our lab technicians have not validated anti-FGFR1 Rabbit Monoclonal antibody M00098 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-FGFR1 Rabbit Monoclonal antibody M00098 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey plasma in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-12-18

Question

Would anti-FGFR1 Rabbit Monoclonal antibody M00098 work for WB with pancreas?

Verified Customer

Verified customer

Asked: 2017-07-12

Answer

According to the expression profile of pancreas, FGFR1 is highly expressed in pancreas. So, it is likely that anti-FGFR1 Rabbit Monoclonal antibody M00098 will work for WB with pancreas.

Boster Scientific Support

Answered: 2017-07-12

Question

Our lab used your anti-FGFR1 Rabbit Monoclonal antibody for IF on placenta in the past. I am using human, and We intend to use the antibody for WB next. My question regards examining placenta as well as plasma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2017-06-30

Answer

I looked at the website and datasheets of our anti-FGFR1 Rabbit Monoclonal antibody and it seems that M00098 has been tested on human in both IF and WB. Thus M00098 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-06-30

Question

Will M00098 anti-FGFR1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

N. Roberts

Verified customer

Asked: 2017-04-20

Answer

It shows on the product datasheet, M00098 anti-FGFR1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-04-20

Question

I have attached the WB image, lot number and protocol we used for pancreas using anti-FGFR1 Rabbit Monoclonal antibody M00098. Please let me know if you require anything else.

K. Bhatt

Verified customer

Asked: 2016-02-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-02-16

Question

I see that the anti-FGFR1 Rabbit Monoclonal antibody M00098 works with WB, what is the protocol used to produce the result images on the product page?

E. Johnson

Verified customer

Asked: 2013-09-19

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-09-19

Order DetailsPrice
M00098

100μL

$370
M00098-30ul

30μL sample

$99
M00098-carrier-free

Carrier Free

$370

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M00098
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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