Click image to see more details
Product Info Summary
| SKU: | M01258 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-FHL1 Monoclonal Antibody
SKU/Catalog Number
M01258
BM5701 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FHL1 Monoclonal Antibody catalog # M01258. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FHL1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01258)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFCI-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FHL1
Reactive Species
M01258 is reactive to FHL1 in Human, Mouse, Rat
Observed Molecular Weight
30 kDa
Calculated molecular weight
36.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01258 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:50
Positive Control
WB: Human fetal muscle
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of FHL1 expression in Human fetal muscle lysate.
Specific Publications For Anti-FHL1 Monoclonal Antibody (M01258)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-FHL1 Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-FHL1 Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-FHL1 Monoclonal Antibody
Question
What is the sample target on Western blot analysis in your product image for M01258?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-FHL1 Monoclonal Antibody (M01258) expression in Human fetal muscle lysate.
Boster Scientific Support
Answered: 2019-12-10
Question
I see that the anti-FHL1 Monoclonal antibody M01258 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-05
Question
Will anti-FHL1 Monoclonal antibody M01258 work on zebrafish WB with brain?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
Our lab technicians have not validated anti-FHL1 Monoclonal antibody M01258 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-FHL1 Monoclonal antibody M01258 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-20
Question
I have a question about product M01258, anti-FHL1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01258 anti-FHL1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-24
Question
Is this M01258 anti-FHL1 Monoclonal antibody reactive to the isotypes of FHL1?
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
The immunogen of M01258 anti-FHL1 Monoclonal antibody is A synthesized peptide derived from human FHL1 May have an involvement in muscle development or hypertrophy. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-26
Question
Does M01258 anti-FHL1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
As indicated on the product datasheet, M01258 anti-FHL1 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-23
Question
I am looking for to test anti-FHL1 Monoclonal antibody M01258 on rat bone marrow for research purposes, then I may be interested in using anti-FHL1 Monoclonal antibody M01258 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Singh
Verified customer
Asked: 2016-04-15
Answer
The products we sell, including anti-FHL1 Monoclonal antibody M01258, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-04-15
