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Product Info Summary
| SKU: | A14099 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-MYBPC2 Antibody
SKU/Catalog Number
A14099
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MYBPC2 Antibody (Catalog # A14099). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.
Storage & Handling
MYBPC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-MYBPC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14099)
Host
Rabbit
Contents
MYBPC2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MYBPC2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human MYBPC2. The immunogen is located within amino acids 1070 - 1120 of MYBPC2.
Reactive Species
A14099 is reactive to MYBPC2 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
128.1 kDa
Background of MYBPC2
Myosin binding protein C (MYBPC) is a component of the thick filament of striated muscle, with the fast-type isoform designated MYBPC2. Both the fast-type and slow-type MYBPC protein contains seven immunoglobulin C2 motifs and three fibronectin type-III repeats. MYBPC2 is typically required for strong contractions and functions under anaerobic conditions. It is more similar to the cardiac isoform (MYBPC3) than to the slow-type isoform (MYBPC1) in terms of pCa50-indexed force development, length-independent cooperativity and length dependent activation. It has been suggested that in cardiac and fast muscle MYBPC2 contributes to an internal load, possibly by binding to actin via its N-terminal region.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14099 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MYBPC2 antibody can be used for detection of MYBPC2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MYBPC2 in mouse skeletal muscle tissue lysate with MYBPC2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of MYBPC2 in mouse skeletal muscle tissue with MYBPC2 antibody at 5 μg/mL.
Specific Publications For Anti-MYBPC2 Antibody (A14099)
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