Anti-FMO3 Antibody

Rabbit IgG polyclonal antibody for Dimethylaniline monooxygenase[N-oxide-forming] 3(FMO3) detection. Tested with WB in Human;Mouse;Rat.

SKU PA1764
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-FMO3 Antibody
See all FMO3 primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1764
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Polyclonal antibody for FMO3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. FMO3 information: Molecular Weight: 60033 MW; Subcellular Localization: Microsome membrane. Endoplasmic reticulum membrane; Tissue Specificity: Liver.
Cite This Product Anti-FMO3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1764)
Specificity Anti-FMO3 Antibody (PA1764) reacts with Human, Mouse, Rat FMO3, in native form and recombinant. Superfamily members of FMO3 are not reactive to PA1764.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human FMO3(76-90aa DDFPNFMHNSKIQEY), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-FMO3 Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-FMO3 Antibody (PA1764).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

/antibody/pa1764-1-WB-anti-fmo3-antibody.jpg

Anti-FMO3 antibody, PA1764, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Liver Tissue Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P31513
Gene Name FMO3
Protein Name Dimethylaniline monooxygenase [N-oxide-forming] 3
Tissue Specificity Liver.
Alternative Names Dimethylaniline monooxygenase [N-oxide-forming] 3;1.14.13.8;Dimethylaniline oxidase 3;FMO II;FMO form 2;Hepatic flavin-containing monooxygenase 3;FMO 3;Trimethylamine monooxygenase;1.14.13.148;FMO3;
Subcellular Localization Microsome membrane. Endoplasmic reticulum membrane.
Molecular Weight 60033 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. It N-oxygenates primary aliphatic alkylamines as well as secondary and tertiary amines. Plays an important role in the metabolism of trimethylamine (TMA), via the production of TMA N-oxide (TMAO). Is also able to perform S-oxidation when acting on sulfide compounds (PubMed:9224773). .
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background FMO3(Flavin-containing Monooxygenase 3) is an enzyme that in humans is encoded by the FMO3 gene. The mammalian flavin-containing monooxygenases(FMO) represent a multigene family whose gene products are localized in the endoplasmic reticulum of many tissues. The FMO3 gene contains 1 noncoding and 8 coding exons. The FMO3 gene is mapped on 1q24.3. Using quantitative RNase protection assays, FMO3 is present in low abundance in fetal liver and lung and in adult kidney and lung, and in much greater abundance in adult liver. By Western blot analysis of human liver microsomal samples ranging from 8 weeks gestation to 18 years of age, FMO1 is the major fetal isoform and FMO3 is the major adult isoform. FMO3 was expressed at intermediate levels until 11 years of age when a gender-independent increase in FMO3 expression was observed during puberty. Sufferers of trimethylaminuria may display a reduced ability to metabolize substrates for FMO3 such as nicotine. FMO3 metabolizes a number of drugs, including amphetamine, clozapine, deprenyl, metamphetamine, tamoxifen, ethionamide, thiacetazone, and sulindac sulfide.

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In stock
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PA1764
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

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Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
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