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Product Info Summary
| SKU: | PA2108 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FMO5 Antibody Picoband®
SKU/Catalog Number
PA2108
BA3717 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FMO5 Antibody catalog # PA2108. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FMO5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2108)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FMO5, different from the related rat sequence by one amino acid, and different from the related mouse sequence by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2108 is reactive to FMO5 in Human
Observed Molecular Weight
60 kDa
Calculated molecular weight
60.2 kDa
Background of FMO5
Dimethylaniline monooxygenase[N-oxide-forming] 5 also known as FMO 5 is an enzyme that in humans is encoded by the FMO5 gene. By fluorescence in situ hybridization, this gene was assigned to human chromosome 1q21.1. FMO5 is not an efficient drug-metabolizing enzyme and that it may have an alternative physiologic role. FMO5 transcripts play an important role in progesterone-regulated in breast cancer cells specifically under the control of the progesterone receptor B-isoform.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2108 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human HepG2 whole cell
Validation Images & Assay Conditions
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Western blot analysis of FMO5 using anti-FMO5 antibody (PA2108).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO5 antigen affinity purified polyclonal antibody (Catalog # PA2108) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMO5 at approximately 60 kDa. The expected band size for FMO5 is at 60 kDa.
Specific Publications For Anti-FMO5 Antibody Picoband® (PA2108)
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Customer Q&As
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4 Customer Q&As for Anti-FMO5 Antibody Picoband®
Question
Is a blocking peptide available for product anti-FMO5 antibody (PA2108)?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
We do provide the blocking peptide for product anti-FMO5 antibody (PA2108). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-12
Question
Would anti-FMO5 antibody PA2108 work on bovine WB with brain?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
Our lab technicians have not tested anti-FMO5 antibody PA2108 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-FMO5 antibody PA2108 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-21
Question
Will anti-FMO5 antibody PA2108 work for WB with trachea?
Verified Customer
Verified customer
Asked: 2017-11-07
Answer
According to the expression profile of trachea, FMO5 is highly expressed in trachea. So, it is likely that anti-FMO5 antibody PA2108 will work for WB with trachea.
Boster Scientific Support
Answered: 2017-11-07
Question
We are currently using anti-FMO5 antibody PA2108 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
F. Carter
Verified customer
Asked: 2016-07-15
Answer
The anti-FMO5 antibody (PA2108) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-15
