Product Info Summary
SKU: | A02831-5 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-FOXE1 Picoband™ Antibody
SKU/Catalog Number
A02831-5
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FOXE1 Picoband™ Antibody catalog # A02831-5. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FOXE1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02831-5)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FOXE1 recombinant protein (Position: M1-M373).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02831-5 is reactive to FOXE1 in Human
Applications
A02831-5 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
41 kDa
Calculated molecular weight
38.076kDa
Background of FOXE1
Forkhead box protein E1 is a protein that in humans is encoded by the FOXE1 gene. It is mapped to 9q22.33. This intronless gene encodes a protein that belongs to the forkhead family of transcription factors. Members of this family contain a conserved 100-amino acid DNA-binding 'forkhead' domain. The encoded protein functions as a thyroid transcription factor that plays a role in thyroid morphogenesis. Mutations in this gene are associated with the Bamforth-Lazarus syndrome, and with susceptibility to nonmedullary thyroid cancer-4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FOXE1 using anti-FOXE1 antibody (A02831-5).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXE1 antigen affinity purified polyclonal antibody (Catalog # A02831-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXE1 at approximately 41KD. The expected band size for FOXE1 is at 38KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of SiHa cells using anti-FOXE1 antibody (A02831-5).
Overlay histogram showing SiHa cells stained with A02831-5 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXE1 Antibody (A02831-5, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For FOXE1 (Source: Uniprot.org, NCBI)
Gene Name
FOXE1
Full Name
Forkhead box protein E1
Weight
38.076kDa
Alternative Names
FKHL15HFKL5; forkhead box E1 (thyroid transcription factor 2); forkhead box E2; Forkhead box protein E2; forkhead, drosophila, homolog-like 15; Forkhead-related protein FKHL15; HFKH4Thyroid transcription factor 2; TITF2HNF-3/fork head-like protein 5; TTF2forkhead box protein E1; TTF-2FOXE2 FOXE1 FKHL15, FOXE2, HFKH4, HFKL5, NMTC4, TITF2, TTF-2, TTF2 forkhead box E1 forkhead box protein E1|HNF-3/fork head-like protein 5|forkhead box protein E2|forkhead, drosophila, homolog-like 15|forkhead-related protein FKHL15|thyroid transcription factor 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXE1, check out the FOXE1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FOXE1 Picoband™ Antibody (A02831-5)
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