Anti-FOXO3A Antibody Picoband™

Boster Bio Anti-FOXO3A Antibody Picoband™ catalog # PB9196. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: PB9196
Size: 100μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-FOXO3A Antibody Picoband™

See all FOXO3 products

SKU/Catalog Number

PB9196

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FOXO3A Antibody Picoband™ catalog # PB9196. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FOXO3A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9196)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human FOXO3A recombinant protein (Position: Q471-G673). Human FOXO3A shares 97% amino acid (aa) sequence identity with mouse FOXO3A.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9196 is reactive to FOXO3 in Human, Rat

Applications

PB9196 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Gene/Protein Information For FOXO3 (Source: Uniprot.Org, NCBI)

Uniprot ID

O43524

Gene ID

2309

Gene Name

FOXO3

Full Name

Forkhead box protein O3

Weight

71.277kDa

Alternative Names

AF6q21 protein; AF6q21; DKFZp781A0677; FKHRL1; FKHRL1MGC31925; FKHRL1P2; forkhead box O3; forkhead box protein O3; forkhead homolog (rhabdomyosarcoma) like 1; Forkhead in rhabdomyosarcoma-like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1; FOXO2; FoxO3; FOXO3A; FOXO3Aforkhead box O3A; MGC12739 FOXO3 AF6q21, FKHRL1, FKHRL1P2, FOXO2A, FOXO3 forkhead box O3 forkhead box protein O3|forkhead box O3A|forkhead homolog (rhabdomyosarcoma) like 1|forkhead in rhabdomyosarcoma-like 1|forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FOXO3, check out the FOXO3 Infographic

FOXO3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FOXO3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Anti-FOXO3A Antibody Picoband™

Question

We are currently using anti-FOXO3A antibody PB9196 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

The anti-FOXO3A antibody (PB9196) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-30

Question

I am interested in using your anti-FOXO3A antibody for signaling by nodal studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

Thanks for your inquiry. This PB9196 anti-FOXO3A antibody is tested on rat thymus tissue, tissue lysate, nrk whole cell lysate, hepg2 whole cell lysate, hela whole cell lysate, k562 whole cell lysate, jurkat whole cell lysate, intestinal cancer tissue, lung cancer tissue. It is guaranteed to work for IHC, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-12

Question

Our lab used your anti-FOXO3A antibody for IHC on cervix carcinoma in the past. I am using rat, and We want to use the antibody for WB next. My question regards examining cervix carcinoma as well as stomach in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-09-28

Answer

I looked at the website and datasheets of our anti-FOXO3A antibody and it seems that PB9196 has been validated on rat in both IHC and WB. Thus PB9196 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-09-28

Question

We have been able to see staining in human stomach. Any tips? Is anti-FOXO3A antibody supposed to stain stomach positively?

Verified Customer

Verified customer

Asked: 2017-09-28

Answer

From literature stomach does express FOXO3. From Uniprot.org, FOXO3 is expressed in trabecular bone tissue, rhabdomyosarcoma, stomach, muscle placenta, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have FOXO3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Muscle, and Placenta, Pubmed ID: 15489334
Rhabdomyosarcoma, Pubmed ID: 9479491
Stomach, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2017-09-28

Question

My colleagues were content with the WB result of your anti-FOXO3A antibody. However we have observed positive staining in cervix carcinoma cytosol using this antibody. Is that expected? Could you tell me where is FOXO3 supposed to be expressed?

J. Roberts

Verified customer

Asked: 2015-05-14

Answer

According to literature, cervix carcinoma does express FOXO3. Generally FOXO3 expresses in cytoplasm, cytosol. Regarding which tissues have FOXO3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Muscle, and Placenta, Pubmed ID: 15489334
Rhabdomyosarcoma, Pubmed ID: 9479491
Stomach, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2015-05-14

Conjugation

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PB9196
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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