Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin)

Product Name

Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin)

View all GAPDH Antibodies

SKU/Catalog Number

M00227-Biotin

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin) (Catalog # M00227-Biotin). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Rabbit.

Storage & Handling

Biotin-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-Biotin)

Host

Mouse

Contents

Biotin-GAPDH antibody is supplied in PBS containing 1% stabilizing protein and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

Clone: 12D3D5

Isotype

IgG

Immunogen

Biotin-GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.

Cross-reactivity

Biotin-GAPDH antibody is human, mouse, rat, and rabbit reactive.

Reactive Species

M00227-Biotin is reactive to GAPDH in Human, Mouse, Rabbit, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

36.1 kDa

Background of GAPDH

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00227-Biotin is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information GAPDH

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