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- Table of Contents
GAPDH antibody (M00227-Biotin) performs well in ELISA, WB and shows reactivity with Human, Mouse, Rabbit, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of GAPDH in multiple cell and tissue lysates with Biotin-GAPDH antibody at 1 μg/ml. Lanes 1-20: 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT, 3T3, Raji, Ramos, U937, human brain, mouse brain, rat brain, rabbit brain, mouse lung, mouse liver, and rat liver, respectively.
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Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin)
GAPDH antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rabbit, Rat | Mouse | ELISA, WB |
Recommend Dilution
Application
Dilution
Species
Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human
mouse
and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin)
View all GAPDH Antibodies
View all GAPDH Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Biotin-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Host
Mouse
Contents
Biotin-GAPDH antibody is supplied in PBS containing 1% stabilizing protein and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 12D3D5
Isotype
IgG
Immunogen
Biotin-GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.
Cross-Reactivity
Biotin-GAPDH antibody is human, mouse, rat, and rabbit reactive.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
36.1 kDa
Background of GAPDH
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Cite This Product
Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-Biotin)
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Product Promise
We promise Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin) performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00227-Biotin
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026