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- Table of Contents
GAPDH antibody (M00227) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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Immunofluorescent analysis of Hela cells, using GAPDH Antibody .
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Anti-GAPDH Rabbit Monoclonal Antibody
GAPDH antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
GAPDH antibody was positively detected by IHC in:
Rat colon, Mouse testis, Human uterus, Human kidney tissue, Human pituitary adenoma tissue, Human squamous carcinoma tissue, Human testis cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
GAPDH antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:5000-1:50000
IHC 1:100-1:500
ICC/IF 1:100-1:250
IP 1:50
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
FC
Rat
Recommend
Tested
Recommend
Recommend
Recommend
Mouse
Recommend
Tested
Recommend
Recommend
Recommend
Human
Recommend
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-GAPDH Rabbit Monoclonal Antibody
View all GAPDH Antibodies
View all GAPDH Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BH-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GAPDH
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
36 kDa
Calculated Molecular Weight
36.1 kDa
Cite This Product
Anti-GAPDH Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227)
Protocols
Immunohistochemistry (IHC) protocol for GAPDH antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for GAPDH antibody
Publications
Application:
Species:
Quercetin-loaded exosomes derived from human umbilical cord mesenchymal stem cells alleviate microglia-mediated neuroinflammation via an anti-inflammatory mechanism
Published: 2026-04-21
Journal: INFLAMMOPHARMACOLOGY
Application: WB
Impact Factor: 5.30
Species: Human
PMID: 42014646
Regulation of transglutaminase-2 and related miRNAs expression by calorie restriction in aorta of aging female mice
Published: 2025-08-12
Journal: MOLECULAR BIOLOGY REPORTS
Application: WB
Impact Factor: 2.80
Species: Mouse
PMID: 40794244
High intake of n-6 polyunsaturated fatty acid exacerbates non-alcoholic steatohepatitis by the involvement of multiple metabolic pathways
Published: 2025-06-23
Journal: Frontiers in Nutrition
Application: WB
Impact Factor: 5.10
Species: Rat
PMID: 40626231
Jiawei Erzhiwan Ameliorates Androgenetic Alopecia by Regulating the SIRT1/JNK/p38 MAPK Pathway
Published: 2025-03-31
Journal: Drug Design Development and Therapy
Application: WB
Impact Factor: 5.10
Species: Mouse
PMID: 40190813
Matrix Gla protein suppresses osteoblast senescence and promotes osteogenic differentiation by the PI3K-AKT signaling pathway
Published: 2024-11-12
Journal: EXPERIMENTAL CELL RESEARCH
Application: WB
Impact Factor: 3.30
Species: Mouse
PMID: 39536932
Autophagy defects at weaning impair complement-dependent synaptic pruning and induce behavior deficits
Published: 2024-09-27
Journal: Journal of Neuroinflammation
Application: WB
Impact Factor: 9.30
Species: Rat
PMID: 39334475
Inhibition of Notch3/Hey1 ameliorates peribiliary hypoxia by preventing hypertrophic hepatic arteriopathy in biliary atresia progression
Published: 2024-04-10
Journal: HISTOCHEMISTRY AND CELL BIOLOGY
Application: WB
Impact Factor: 2.10
Species: Human,Mouse
PMID: 38597939
Kisspeptin-10 Administration Regulates mTOR and AKT Activities and Oxidative Stress in Mouse Cardiac Tissue
Published: 2021-05-06
Journal: JOURNAL OF EVOLUTIONARY BIOCHEMISTRY AND PHYSIOLOGY
Application: WB
Impact Factor: 0.44
Species: Mouse
Effective robotic assistive pattern of treadmill training for spinal cord injury in a rat model
Published: 2018-01-31
Journal: Experimental and Therapeutic Medicine
Application: WB
Impact Factor: 1.41
Species: Rat
PMID: 29545846
Engrailed‑2 promoter hyper‑methylation is associated with its downregulation in clear cell renal cell carcinoma
Published: 2017-09-19
Journal: Oncology Letters
Application: WB
Impact Factor: 1.39
Species: Human
PMID: 29151918
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Product Promise
We promise Anti-GAPDH Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00227
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026