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GAPDH antibody (M00227-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 80 Publications

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m00227 1 gapdh primary antibodies if testing 1

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Immunofluorescent analysis of Hela cells, using GAPDH Antibody.

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Anti-GAPDH Rabbit Monoclonal Antibody

GAPDH antibody

80 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

GAPDH antibody was positively detected by WB in:

human Hela whole cell, human K562 whole cell, human MCF-7 whole cell, human A549 whole cell, human PC-3 whole cell, monkey COS-7 whole cell, rat liver tissue, rat brain tissue, rat RH35 whole cell, mouse liver tissue, mouse brain tissue, mouse NIH/3T3 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

GAPDH antibody was positively detected by IHC in:

human bladder cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ICC

Human

Tested

Recommend

Monkey

Tested

Rat

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-GAPDH Rabbit Monoclonal Antibody
View all GAPDH Antibodies

Form

Liquid

Uniprot

P04406

Gene ID

2597

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

BG-7

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human GAPDH

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

36 kDa

Calculated Molecular Weight

36.1 kDa

Cite This Product

Anti-GAPDH Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-1)

Protocols

Western Blot protocol for GAPDH antibody

Review Protocol

Immunohistochemistry (IHC) protocol for GAPDH antibody

Review Protocol

Publications

Cordycepin protects against simulated microgravity–induced osteogenic impairment involving ferroptosis-related signaling

Published: 2026-08-16

Journal: BIOCHEMICAL PHARMACOLOGY

Application: WB

Impact Factor: 6.50

Species: Mouse

PMID: 42603620

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Free Secondary Antibody

Anti-GAPDH Rabbit Monoclonal Antibody

GAPDH antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Cordycepin protects against simulated microgravity–induced osteogenic impairment involving ferroptosis-related signaling

Experimental Study of YaJieShaBa Antialcoholic Hepatic Fibrosis Through TGF‐β1/Smad Signaling Pathway

rRGD3mu, a Triple-RGD Recombinant Peptide, Suppresses Malignant Phenotypes in Nasopharyngeal Carcinoma-Associated Models Through the Modulation of ITGB1-Associated FAK/AKT Signaling

Enhanced gastrointestinal stability and therapeutic efficacy of biofilm self-coated Bacillus amyloliquefaciens C-1 probiotic in ulcerative colitis

β-Ecdysterone Attenuates Ang II–Induced Senescence in Human Aortic Smooth Muscle Cells via Autophagy Activation and ROS Suppression Through AKT/mTOR Pathway Inhibition

The novel GLP-1/GIP dual receptor agonist DA5-CH is superior to tirzepatide and exendin-4 in the 6-OHDA Parkinson rat model

Black Phosphorus Nanosheets for Enhanced Osteogenic Differentiation of Dental Follicle Stem Cells

SA4503 plays a protective role in post-resuscitation injury by reducing apoptosis caused by mitochondrial dysfunction and endoplasmic reticulum stress through the activation of sigma-1 receptor

In situ coagulation environment regulation–assisted thrombus clearance via hydrogenated silicon-based nanothrombolytics

Bacillus amyloliquefaciens Fmb50 Alleviates Hyperglycemia by Reshaping Gut Structures and Mitigating Metabolic Disorders in Type 2 Diabetic Mice

Therapeutic potential of Dahuang Mudan Decoction for severe acute pancreatitis: targeting oxidative stress and MAPK pathways through network pharmacology and experimental verification1

Promoting Drp1-Mediated Mitochondrial Division in Nickel Nanoparticles-Induced Reproductive Toxicity in GC-2 Cells

TNIP3 Attenuates Colitis by Inhibiting the TAB1/NF-κB/NLRP3 Signaling Pathway in Macrophages

The Effects of Da-Chai-Hu Decoction Alleviating Pancreatic Exocrine Dysfunction by Inhibiting Endoplasmic Reticulum Stress of Acinar Cell in Mice with Chronic Pancreatitis

Electroacupuncture Prevents Against AD‐Like Phenotypes in APP/PS1 Mice: Investigation of the Mechanisms From Cerebral Microangiopathy

Adrenal injury induced by real-ambient PM2.5: Mechanisms of disrupted Shh/Wnt signaling crosstalk

Mulberry leaf flavonoid extract inhibits high glucose-induced ferroptosis by regulating the PI-3K/Akt/Nrf2/GPX4 pathway in HT22 mouse hippocampal neuronal cells

SASH1 Modulates Melanin Synthesis and Melanoma Cell Metastasis via Suppression of the TGF-β Signaling Pathway

CXCL10 Involvement in Vestibular Migraine via the PI3K/AKT Signaling Pathway

Circular RNA Gsr-0002 promotes schistosomiasis-induced liver fibrosis by regulating the DNMT3A/PTEN pathway

PLIN2 exacerbates Allergic Rhinitis by inhibiting PINK1/Parkin-mediated mitophagy

Redox-sensitive hyaluronic acid-based glycopeptide nanoparticles: Dual-action platform for mitochondrial disruption and tumor suppression

N6-methyladenosine attenuates tumor-associated macrophages M2 polarization via suppressing the translation of Snail

Chrysin inhibits PEDV replication by antagonizing apoptosis via the ROS/JNK/p53 axis

Plumbagin targets the GLUT1/MMP-2 axis to inhibit oral squamous cell carcinoma progression

Hsa_circ_0081111 promotes the proliferation and metastasis of non-small cell lung cancer by regulating IGF2BP2-mediated stability of Slug mRNA

Structural characterization and effect of activating autophagy and regulating oxidative stress of polysaccharide from fibrous roots of Atractylodes chinensis

Arctiin, a lignan compound, enhances adipose tissue browning and energy expenditure by activating the adenosine A2A receptor

Rictor stability mediated by USP9X regulates embryo implantation by participating in lipid metabolism of endometrium

FKBP5 mediates glucocorticoid signaling in estrogen deficiency-associated endothelial dysfunction

Echinococcus granulosus sensu lato promotes osteoclast differentiation through DUSP4-MAPK signaling in osseous echinococcosis

Multifunctional nanoparticles for immune regulation and oxidative stress alleviation in myocarditis

Gut microbiota-derived tryptophan metabolites improve total parenteral nutrition-associated infections by regulating Group 3 innate lymphoid cells

VPS45 Contributes to the Progression of Hepatocellular Carcinoma by Triggering the Wnt/β-Catenin Signaling Pathway

Identifying Hepsin as a novel biomarker for human esophageal squamous cell carcinoma (ESCC) and its application in fluorescence imaging

“Convex Lens” of DNA damage: A nanomedicine enhances anti-PD-1 immunotherapy as immunogenic cell death inducer for “cold” melanoma

Extracellular vesicles derived from dental follicle stem cells regulate tooth eruption by inhibiting osteoclast differentiation

The Role of the Cytoskeletal Regulatory Protein, Mammalian Enabling Protein (Mena), in Invasion and Metastasis of HPV16-Related Oral Squamous Cell Carcinoma

Alginate-based functionalized, remote, light-responsive hydrogel transducer for synergistic mild photo thermoelectric stimulation for tumor therapy

Screening of active components of melastoma dodecandrum lour. against diabetic osteoporosis using cell membrane chromatography-mass spectrometry

Blestriarene C exerts an inhibitory effect on triple-negative breast cancer through multiple signaling pathways

The Protective Role of Intermedin in Contrast-Induced Acute Kidney Injury: Enhancing Peritubular Capillary Endothelial Cell Adhesion and Integrity Through the cAMP/Rac1 Pathway

Interleukin-11Rα2 in the hypothalamic arcuate nucleus affects depression-related behaviors and the AKT-BDNF pathway

Identifying key components from Melastoma dodecandrum in TNF-α-induced osteoblast injury model through a combination of cell membrane chromatography and mass spectrometry

Lentivirus-mediated RNA interference targeting HMGB1 modulates AQP1 to reduce pain induced by chronic compression of the dorsal root ganglia

The role of AdipoQ on proliferation, apoptosis, and hormone Secretion in chicken primary adenohypophysis cells

Expression of the laminin genes family and its relationship to prognosis in pancreatic carcinoma

Methylprednisolone Modulates the Tfr/Tfh ratio in EAE-Induced Neuroinflammation through the PI3K/AKT/FoxO1 and PI3K/AKT/mTOR Signalling Pathways

Mechanism underlying the role of integrin α3β1 in adhesive dysfunction between thyroid cells induced by diesel engine exhaust particles

Structural characteristics and anti-tumor effect of low molecular weight Dendrobium officinale polysaccharides by reconstructing tumor microenvironment

Role of transforming growth factor-β1 pathway in angiogenesis induced by chronic stress in colorectal cancer

Loss of SIL1 Affects Actin Dynamics and Leads to Abnormal Neural Migration

Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer

Enhanced therapeutic potential of Flotillins-modified MenSCs by improve the survival, proliferation and migration

Circ_0002331 Interacts with ELAVL1 to Improve ox-LDL-Induced Vascular Endothelial Cell Dysfunction via Regulating CCND2 mRNA Stability

Tamarix aphylla derived metabolites ameliorate indomethacin-induced gastric ulcers in rats by modulating the MAPK signaling pathway, alleviating oxidative stress and inflammation: In vivo study supported by pharmacological network analysis

Ruthenium Red Alleviates Post-Resuscitation Myocardial Dysfunction by Upregulating Mitophagy through Inhibition of USP33 in a Cardiac Arrest Rat Model

Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study

Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway

Exploring the potential of white birch sap: A natural alternative to traditional skin whitening agents with reduced side effects

Porphyromonas gingivalis promotes the progression of oral squamous cell carcinoma by stimulating the release of neutrophil extracellular traps in the tumor immune microenvironment

The mediating role of AKT/ERK/JNK signaling on the malignant phenotype of microcystin-LR in gastric adenocarcinoma cells

(2E)-1-(2,4,6-Trimethoxyphenyl)-3-(4-chlorophenyl)prop-2-en-1-one, a Chalcone Derivative, Promotes Apoptosis by Suppressing RAS-ERK and AKT/FOXO3a Pathways in Hepatocellular Carcinoma Cells

Role and mechanism of Circ-PDE7B in the formation of keloid

TRIM55 inhibits colorectal cancer development via enhancing protein degradation of c-Myc

Mena as a key enhancer factor of EMT to promote metastasis of human tongue squamous cell carcinoma

Reduction of SLC7A11 and GPX4 Contributing to Ferroptosis in Sperm from Asthenozoospermia Individuals