Anti-GAPDH Rabbit Monoclonal Antibody

Boster Bio Anti-GAPDH Rabbit Monoclonal Antibody catalog # M00227-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Chicken, Cow, Dog, Human, Monkey, Mouse, Rat. Cited in 150 publication(s).

Product Info Summary

SKU: M00227-1
Size: 100 μl
Reactive Species: Chicken, Cow, Dog, Human, Monkey, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-GAPDH Rabbit Monoclonal Antibody

See all GAPDH products

SKU/Catalog Number

M00227-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-GAPDH Rabbit Monoclonal Antibody catalog # M00227-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Chicken, Cow, Dog, Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GAPDH Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BG-7

Immunogen

A synthesized peptide derived from human GAPDH

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00227-1 is reactive to GAPDH in Chicken, Cow, Dog, Human, Monkey, Mouse, Rat

Applications

M00227-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:3000-1:10000
IHC 1:100-1:250
ICC/IF 1:100-1:250

Validation Images & Assay Conditions

Gene/Protein Information For GAPDH (Source: Uniprot.Org, NCBI)

Uniprot ID

P04406

Gene ID

2597

Gene Name

GAPDH

Full Name

Glyceraldehyde-3-phosphate dehydrogenase

Weight

36.053kDa

Superfamily

glyceraldehyde-3-phosphate dehydrogenase family

Alternative Names

1D4 GAPDH; aging-associated gene 9 protein; Cytoplasm Marker; EC 1.2.1; EC 1.2.1.12; EC 2.6.99.-; G3PD; G3PD; G3PDH; GAPD; GAPDH; GAPDPeptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38; OCT1 coactivator in S phase; Peptidyl-cysteine S-nitrosylase GAPDH GAPDH G3PD, GAPD, HEL-S-162eP glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase|OCAS, p38 component|Oct1 coactivator in S phase, 38 Kd component|aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GAPDH, check out the GAPDH Infographic

GAPDH infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GAPDH: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00227-1 has been cited in 150 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang J,Fang Y,Liu YF,Wang X,Wang XL,Wang RY,Meng ZD.MiR-154 inhibits cells proliferation and metastasis in melanoma by targeting AURKA and serves as a novel prognostic indicator.Eur Rev Med Pharmacol Sci.2019 May;23(10):4275-4284.doi:10.26355/eurrev_201905_17932.PMID:31173299.
Species: Human
M00227-1 usage in article: APP:WB, SAMPLE:A375 CELL, DILUTION:1:1500

Duan Y,Tan Z,Yang M,Li J,Liu C,Wang C,Zhang F,Jin Y,Wang Y,Zhu L.PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF-κB Signaling Pathway.Biomed Res Int.2019 Apr 1;2019:8650846.doi:10.1155/2019/8650846. PMID:31058194;PMCID:PMC6463683.
Species: Human
M00227-1 usage in article: APP:WB, SAMPLE:OSTEOCLASTS, DILUTION:NA

Huang W,Guo L,Zhao M,Zhang D,Xu H,Nie Q.The Inhibition on MDFIC and PI3K/AKT Pathway Caused by miR-146b-3p Triggers Suppression of Myoblast Proliferation and Differentiation and Promotion of Apoptosis.Cells.2019 Jun 29;8(7):656.doi:10.3390/cells8070656.PMID:31261950; PMCID:PMC6678156.
Species: Chicken
M00227-1 usage in article: APP:WB, SAMPLE:QM-7 CELL, DILUTION:1:2000

Xiang W,Jiang T,Hao X, Wang R,Yao X,Sun K,Guo F,Xu T.Primary cilia and autophagy interaction is involved in mechanical stress mediated cartilage development via ERK/mTOR axis.Life Sci.2019 Feb 1;218:308-313.doi:10.1016/j. lfs.2019.01.001.Epub 2019 Jan 3.PMID:30610869.
Species: Rat
M00227-1 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:1:400

Sheng X,Li Y,Li Y,Liu W,Lu Z,Zhan J,Xu M,Chen L,Luo X,Cai G,Zhang S.PLOD2 contributes to drug resistance in laryngeal cancer by promoting cancer stem cell-like characteristics.BMC Cancer.2019 Aug 27;19(1):840.doi:10.1186/s12885-019-6029-y.PMID:31455288; PMCID:PMC6712771.
Species: Human,Mouse
M00227-1 usage in article: APP:WB, SAMPLE:FADU CELL,HEP2 CELL AND LSCC TISSUE, DILUTION:1:3000

Li Z,Feng J,Yang S,Meng P,Li J,Li H,Gao X,Zhang Y.Lipopolysaccharide-induced inflammation in human peritoneal mesothelial cells is controlled by ERK1/2-CDK5-PPARγ axis.Ann Transl Med.2021 May;9(10):850.doi:10.21037/atm-21-1623.PMID:34164484;PMCID:PMC8184493.
Species: Human
M00227-1 usage in article: APP:WB, SAMPLE:HMRSV5 CELL, DILUTION:1:10000

Jia Han,Wei Hou,Bi-qing Cai,Fan Zhang,Jian-cai Tang,"12-Epi-Napelline Inhibits Leukemia Cell Proliferation via the PI3K/AKT Signaling Pathway In Vitro and In Vivo",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 6687519,12 pages,2021.https://doi.org/10.1155/2021/6687519
Species: Human,Mouse
M00227-1 usage in article: APP:WB, SAMPLE:K-562 CELL, DILUTION:NA

Enoch Appiah Adu-Gyamfi,Jones Lamptey,Xue-Mei Chen,Fang-Fang Li,Cong Li,Ling-Ling Ruan,Xue-Niu Yang,Tai-Hang Liu,Ying-Xiong Wang,Yu-Bin Ding,Iodothyronine deiodinase 2 (DiO2) regulates trophoblast cell line cycle, invasion and apoptosis; and its downregulation is associated with early recurrent miscarriage,Placenta,Volume 111,2021,Pages 54-68,ISSN 0143-4004,https://doi.org/10.1016/j.placenta.2021.06.004.
Species: Human
M00227-1 usage in article: APP:WB, SAMPLE:HTR-8/SVNEO CELL, DILUTION:NA

Tibatan MA,Önay Uçar E.Long non-coding RNA NKILA regulates expression of HSP90α, NF-κB and β-catenin proteins in the MCF-7 breast cancer cell line.Mol Biol Rep.2021 Jun 14.doi:10.1007/s11033-021-06482-y.Epub ahead of print.PMID:34125329.
Species: Human
M00227-1 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:NA

Zhao,Y.,Jia,W.,Ren,S.,Xiao,W.,Li,G.,Jin,L.,& Lin,Y.(2021). Difluoromethylornithine attenuates isoproterenol‑induced cardiac hypertrophy by regulating apoptosis, autophagy and the mitochondria‑associated membranes pathway.Experimental and Therapeutic Medicine,22,870.https://doi.org/10.3892/etm.2021.10302
Species: Rat
M00227-1 usage in article: APP:WB, SAMPLE:VENTRICLE TISSUE, DILUTION:1:1000

Tang,K.,Zhang,W.S.,Liu,Y.,Zhu,W.,& Qiu,J.(2021).Physicochemical properties and in vitro osteocompatibility of different titanium surfaces stored in a saline solution.Materials Research Express.
Species: Mouse
M00227-1 usage in article: APP:WB, SAMPLE:MC3T3-E1 CELL, DILUTION:NA

Xiaoming Zhang,Ying-Hong Tao,Xiu-Ling Zhou et al.The Role of Carbonic Anhydrase Ⅲ and Autophagy in Type 2 Diabetes with Cardio-Cerebrovascular Disease,08 June 2021,PREPRINT(Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-495613/v1]
Species: Mouse
M00227-1 usage in article: APP:WB, SAMPLE:CORTEX AND MYOCARDIUM, DILUTION:1:2000

Ji,M.,Duan,X.,Han,X.,Sun,J.,& Zhang,D.(2021).Exogenous transforming growth factor‑β1 prevents the inflow of fluoride to ameleoblasts through regulation of voltage‑gated chloride channels 5 and 7. Experimental and Therapeutic Medicine,21,615.https://doi.org/10.3892/etm. 2021.10047
Species: A00227-1;APP:WB;SAMPLE:LS8 CELL;DILUTION:1:500|M00227-1;APP:WB;SAMPLE:LS8 CELL;DILUTION:1:500|BA1054;APP:WB;SAMPLE:LS8 CELL;DILUTION:1:5000|BA1060 ;APP:WB;SAMPLE:LS8 CELL;DILUTION:1:5000

Li D,Liu J,Yang C,Tian Y,Yin C,Hu L,Chen Z,Zhao F,Zhang R,Lu A,Zhang G,Qian A.Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging.Theranostics.2021 Mar 20;11(11):5585-5604.doi:10.7150/thno.54477. PMID:33859765;PMCID:PMC8039942.
Species: Human,Mouse
M00227-1 usage in article: APP:WB, SAMPLE:HFOB1.19 CELL AND FEMUR TISSUE, DILUTION:1:1000

Guo H,Zhao W,Liu A,Wu M,Shuai Y,Li B,Huang X,Liu X,Yang X,Guo X,Xuan K,Jin Y.SHED promote angiogenesis in stem cell-mediated dental pulp regeneration.Biochem Biophys Res Commun.2020 Sep 3;529(4):1158-1164.doi:10.1016/j.bbrc.2020.06.151.Epub 2020 Aug 3.PMID:32819580.
Species: Human,Pig
M00227-1 usage in article: APP:WB, SAMPLE:R-SHED AND SHED, DILUTION:NA

Shan S,Wu C,Shi J,Zhang X,Niu J,Li H,Li Z.Inhibitory Effects of Peroxidase from Foxtail Millet Bran on Colitis-Associated Colorectal Carcinogenesis by the Blockage of Glycerophospholipid Metabolism.J Agric Food Chem.2020 Aug 5;68(31):8295-8307.doi:10.1021/acs.jafc.0c03257.Epub 2020 Jul 27.PMID:32657580.
Species: Human,Mouse
M00227-1 usage in article: APP:WB, SAMPLE:HCT-116 CELL AND DLD1 CELL, DILUTION:NA

Li Li,Xiao Zhang,Hailong Yang et al.miR-193b-3p Promotes Proliferation of Goat Myoblast Through Directly Targeting IGF2BP1 and Activating Its Transcription,23 October 2020,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-95708/v1]
Species: Goat
M00227-1 usage in article: APP:WB, SAMPLE:MUSCS, DILUTION:NA

Chen M,Lu C,Lu H,Zhang J,Qin D,Liu S,Li X,Zhang L.Farnesoid X receptor via Notch1 directs asymmetric cell division of Sox9+ cells to prevent the development of liver cancer in a mouse model.Stem Cell Res Ther.2021 Apr 12;12(1):232.doi:10.1186/s13287-021-02298-6.PMID:33845903;PMCID:PMC8042944.
Species: Human,Mouse
M00227-1 usage in article: APP:WB, SAMPLE:LIVER TISSUE AND HUH7 CELL, DILUTION:NA

Li L,Zhou C,Li T,Xiao W,Yu M,Yang H.Interleukin-28A maintains the intestinal epithelial barrier function through regulation of claudin-1.Ann Transl Med.2021 Mar;9(5):365.doi:10.21037/atm-20-5494.PMID:33842586;PMCID:PMC8033364.
Species: Human,Mouse
M00227-1 usage in article: APP:WB, SAMPLE:INTESTINE TISSUE AND CACO-2 CELL, DILUTION:1:1000

Wu T,Wang X,Ren K,Huang X,Liu J:The Alterations in Methylene Blue/Light-Treated Frozen Plasma Proteins Revealed by Proteomics.Transfus Med Hemother 2021.doi:10.1159/000515119.
Species: Human
M00227-1 usage in article: APP:WB, SAMPLE:PLASMA, DILUTION:1:300

Zhang Y, Zhou Q, Ding X, Ma J, Tan G. Chemical profile of Swertia mussotii Franch and its potential targets against liver fibrosis revealed by cross-platform metabolomics. J Ethnopharmacol. 2021 Mar 18:114051. doi: 10.1016/j.jep.2021.114051. Epub ahead of print. PMID: 33746001.
Species: Mouse
M00227-1 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA

Wang S,Zhu W,Ouyang L,Li J,Li S,Yang X.Up-Regulation of Tiam1 Promotes the Radioresistance of Laryngeal Squamous Cell Carcinoma Through Activation of the JNK/ATF-2 Signaling Pathway.Onco Targets Ther.2020 Jul 21;13:7065-7074.doi:10.2147/OTT.S257748.PMID:3
Species: Human
M00227-1 usage in article: APP:WB, SAMPLE:TU686 CELL, DILUTION:1:2000

Zhang C,Wang J,Zhu J,Chen Y,Han X.Microcystin-leucine-arginine induced neurotoxicity by initiating mitochondrial fission in hippocampal neurons.Sci Total Environ.2020 Feb 10;703:134702.doi:10.1016/j.scitotenv.2019.134702.Epub 2019 Nov 1.PMID:31753492.
Species: Mouse
M00227-1 usage in article: APP:WB, SAMPLE:HT-22 CELL, DILUTION:1:5000

Ying XIE,Chu-Tian Mai,De-Chong Zheng,Yu-Fei He,Sen-Lin Feng,Ya-Zhou Li,Chang-Xiao Liu,Hua Zhou,Liang Liu,Wutou decoction ameliorates experimental rheumatoid arthritis via regulating NF-kB and Nrf2: Integrating efficacy-oriented compatibility of traditiona
Species: Rat
M00227-1 usage in article: APP:WB, SAMPLE:RAW264.7 CELL AND THP-1 CELL, DILUTION:NA

Ying XIE,Chu-Tian Mai,De-Chong Zheng,Yu-Fei He,Sen-Lin Feng,Ya-Zhou Li,Chang-Xiao Liu,Hua Zhou,Liang Liu,Wutou decoction ameliorates experimental rheumatoid arthritis via regulating NF-kB and Nrf2: Integrating efficacy-oriented compatibility of traditiona
Species: Rat
M00227-1 usage in article: APP:WB, SAMPLE:RAW264.7 CELL AND THP-1 CELL, DILUTION:NA

Chu X,Yu T,Huang X,Xi Y,Ni B,Zhang R,You H.Tomatidine suppresses inflammation in primary articular chondrocytes and attenuates cartilage degradation in osteoarthritic rats.Aging (Albany NY).2020 Jul 5;12(13):12799-12811.doi:10.18632/aging.103222.Epub 2020
Species: Rat
M00227-1 usage in article: APP:WB, SAMPLE:PRIMARY CHONDROCYTES, DILUTION:1:500

Lian Y,Zhu M,Chen J,Yang B,Lv Q,Wang L,Guo S,Tan X,Li C,Bu W,Ding W,Jia X,Feng L. Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy. Int J Biol Macromol. 2021 Feb 10:S0141-8130(2
Species: Human,Rat
M00227-1 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:200

Zhu X,Xue D,Liu J,Dong F,Li Y,Liu Y.Nodal is involved in chemoresistance of renal cell carcinoma cells via regulation of ABCB1.J Cancer 2021;12(7):2041-2049.doi:10.7150/jca.52092.Available from https://www.jcancer.org/v12p2041.htm
Species: Human
M00227-1 usage in article: APP:WB, SAMPLE:GRC-1 CELL AND ACHN, DILUTION:1:1000

Chen X,Xu H,Hou J,Wang H,Zheng Y,Li H,Cai H,Han X,Dai J.Epithelial cell senescence induces pulmonary fibrosis through Nanog-mediated fibroblast activation.Aging (Albany NY).2019 Dec 31;12(1):242-259.doi:10.18632/aging.102613.Epub 2019 Dec 31.PMID:31891567
Species: Human,Mouse
M00227-1 usage in article: APP:WB, SAMPLE:MLE-12 CELL, DILUTION:NA

She Y,Sun J,Hou P,Fang P, Zhang Z. Time-restricted feeding attenuates gluconeogenic activity through inhibition of PGC-1α expression and activity. Physiol Behav. 2021 Mar 15;231:113313. doi: 10.1016/j.physbeh.2021.113313. Epub 2021 Jan 4.PMID:33412190.
Species: Mouse
M00227-1 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA

Wang P,Zhang Y,Wang Z,Yang A,Li Y,Zhang Q.miR-128 regulates epilepsy sensitivity in mice by suppressing SNAP-25 and SYT1 expression in the hippocampus.Biochem Biophys Res Commun.2021 Feb 8;545:195-202.doi:10.1016/j.bbrc.2021.01.079.Epub ahead of print.PMI
Species: Mouse
M00227-1 usage in article: APP:WB, SAMPLE:BRAIN TISSUE AND NEURON CELL, DILUTION:1:2000

Lin X,Chen H,Chen M,Li T,Lai Y,Lin L,Lin P,Liu J,Zhang Y,Chen R,Du H,Jiang X,Liu N.Bone marrow-derived mesenchymal stem cells improve post-ischemia neurological function in rats via the PI3K/AKT/GSK-3β/CRMP-2 pathway.Mol Cell Biochem.2021 Feb 9.doi:10.100
Species: Rat
M00227-1 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:500

Wang R,Li X,Sun C,Yu L,Hua D,Shi C,Wang Q,Rao C,Luo W,Jiang Z,Zhou X,Yu S.The ATPase Pontin is a key cell cycle regulator by amplifying E2F1 transcription response in glioma.Cell Death Dis.2021 Feb 1;12(2):141.doi:10.1038/s41419-021-03421-4.PMID:33542204.
Species: Human
M00227-1 usage in article: APP:WB, SAMPLE:ASTROCYTE GLIOMA TISSUE AND BRAIN TISSUE, DILUTION:NA

Gang-Zhu Xu,Yang Xue,Si-Qi Wei,Jia-Heng Li,Richard J. Traub,Mao-De Wang,Dong-Yuan Cao,Valproate reverses stress-induced somatic hyperalgesia and visceral hypersensitivity by up-regulating spinal 5-HT2C receptor expression in female rats,Neuropharmacology,
Species: Rat
M00227-1 usage in article: APP:WB, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:4000

Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
M00227-1 usage in article: APP:WB, SAMPLE:MSC-LIPO, DILUTION:NA

Hua, D.,Tang, L.,Wang, W.,Tang, S.,Yu, L.,Zhou, X.,Wang, Q.,Sun, C.,Shi, C.,Luo, W.,Jiang, Z.,Li,H.,Yu, S.,Improved Antiglioblastoma Activity and BBB Permeability by Conjugation of Paclitaxel to a Cell‐Penetrative MMP‐2‐Cleavable Peptide. Adv.Sci.2020,200
Species: Human,Mouse
M00227-1 usage in article: APP:WB, SAMPLE:U87MG CELL AND U251 CELL, DILUTION:1:2000

Shan S,Xie Y,Zhang C,Jia B,Li H,Li Z.Identification of polyphenol from Ziziphi spinosae semen against human colon cancer cells and colitis-associated colorectal cancer in mice.Food Funct.2020 Sep 23;11(9):8259-8272.doi:10.1039/d0fo01375g.PMID:32966479.
Species: Human,Mouse
M00227-1 usage in article: APP:WB, SAMPLE:HCT-116 CELL AND HCT-8 CELL, DILUTION:NA

Zhang B,Zhong Q,Chen X,Wu X,Sha R,Song G,Zhang C,Chen X.Neuroprotective Effects of Celastrol on Transient Global Cerebral Ischemia Rats via Regulating HMGB1/NF-κB Signaling Pathway.Front Neurosci.2020 Aug 11;14:847.doi:10.3389/fnins.2020.00847.PMID:328485
Species: Rat
M00227-1 usage in article: APP:WB, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:1000

Li M,Xie X,Chen H,Xiong Q,Tong R,Peng C,Peng F.Aconitine induces cardiotoxicity through regulation of calcium signaling pathway in zebrafish embryos and in H9c2 cells.J Appl Toxicol.2020 Jun;40(6):780-793.doi:10.1002/jat.3943.Epub 2020 Jan 23.PMID:3197543
Species: Zebrafish,Rat

Singh M,George AK,Eyob W,Homme RP,Stanisic D,Tyagi SC.High methionine diet in skeletal muscle remodeling: Epigenetic mechanism of homocysteine mediated growth retardation.Can J Physiol Pharmacol.2020 Aug 15.doi:10.1139/cjpp-2020-0093.Epub ahead of print.P
Species: Mouse
M00227-1 usage in article: APP:WB, SAMPLE:MUSCLE TISSUE, DILUTION:1:1000

Yuan Y,Li B,Kuang Y,Ni S,Zhuge A,Yang J,Lv L,Gu S,Yan R,Li Y,Wang K,Yang L,Zhu X,Wu J,Bian X,Li L. The fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells. Cancer Cell Int.2020 Jun 3;20:212.doi:10. 1186/s12935-020-01305-9
Species: Human,Mouse
M00227-1 usage in article: APP:WB, SAMPLE:HCT-116 CELL AND HT-29 CELL, DILUTION:1:1000

Ni S,Kuang Y,Yuan Y,Yu B. Mitochondrion-mediated iron accumulation promotes carcinogenesis and Warburg effect through reactive oxygen species in osteosarcoma. Cancer Cell Int.2020 Aug 18;20:399. doi:10.1186/s12935-020-01494-3.PMID:32831652;PMCID:PMC743701
Species: Human,Mouse
M00227-1 usage in article: APP:WB, SAMPLE:SAOS-2 CELL AND U2OS CELL, DILUTION:1:1000

Liu L,Liu F,Sun Z,Peng Z,You T,Yu Z. LncRNA NEAT1 promotes apoptosis and inflammation in LPS-induced sepsis models by targeting miR-590-3p. Exp Ther Med.2020 Oct; 20(4):3290-3300. doi:10.3892/etm.2020.9079. Epub 2020 Jul 29.PMID:32855700;PMCID:PMC7444425.
Species: Human
M00227-1 usage in article: APP:WB, SAMPLE:H9C2 CELL, DILUTION:1:2000

Fan YN,Li C,Huang L,Chen L,Tang Z,Han G,Liu Y.Characterization of Group I Metabotropic Glutamate Receptors in Rat and Human Adrenal Glands.Front Physiol.2020 May 27;11:401.doi:10.3389/fphys.2020.00401. PMID:32536873;PMCID:PMC7267184.
Species: Rat,Human
M00227-1 usage in article: APP:WB, SAMPLE:ADRENAL MEDULLA, DILUTION:1:1000

Chen H,Sheng H,Zhao Y,Zhu G.Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway.Med Sci Monit.2020 Dec 31;26:e928403.doi:10.12659/MSM.928403.PMID:33382
Species: Human,Mouse
M00227-1 usage in article: APP:WB, SAMPLE:SNU-16 CELL, DILUTION:NA

Zheng WP,Meng FL,Wang LY.miR-544a Stimulates endometrial carcinoma growth via targeted inhibition of reversion-inducing cysteine-rich protein with Kazal motifs.Mol Cell Probes.2020 Oct;53:101572.doi:10.1016/j. mcp.2020.101572.Epub 2020 Jun 7.PMID:32525042
Species: Human,Mouse
M00227-1 usage in article: APP:WB, SAMPLE:HEC-1B CELLS, DILUTION:1:1000

Guo L,Huang W,Tong F,Chen X,Cao S,Xu H,Luo W,Li Z,Nie Q.Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Host‘s Immune Response to Coccidiosis.Front Physiol.2020 Oct 30;11:573676.doi:10.3389/fphys.2020.573676.PM
Species: Ross 308 commercial broilers
M00227-1 usage in article: APP:WB, SAMPLE:HD11 CELLS, DILUTION:1:2000

Chen Y,Zhang S,Bai C,Guan Z,Chen W.Circ_0000885 Enhances Osteosarcoma Progression by Increasing FGFR1 Expression via Sponging MiR-1294.Cancer Manag Res.2020 Jul 28;12:6441-6452.doi:10.2147/CMAR. S244382.PMID:32801884;PMCID:PMC7395693.
Species: Human, Mouse
M00227-1 usage in article: APP:WB, SAMPLE:SAOS-2 CELLS AND SOSP-9607 CELLS, DILUTION:1:400

Zhang J,Fu ZL,Chu ZX,Song BW.Gastroprotective Activity of the Total Flavones from Abelmoschus manihot (L.) Medic Flowers. Evid Based Complement Alternat Med. 2020 Feb 24;2020:6584945.doi: 10.1155/2020/6584945.PMID:32184895;PMCID:PMC7060849.
Species: Abelmoschus manihot (L.) Medic, Mouse
M00227-1 usage in article: APP:WB, SAMPLE:GASTRIC TISSUE, DILUTION:1:200

Chen L, Wu Q, Xu X, Yang C, You J, Chen F, Zeng Y. Cancer/testis antigen LDHC promotes proliferation and metastasis by activating the PI3K/Akt/GSK-3β-signaling pathway and the in lung adenocarcinoma. Exp Cell Res. 2020 Dec 7:112414. doi: 10.1016/j.yexcr.2
Species: Human, Mouse
M00227-1 usage in article: APP:WB, SAMPLE:A549 CELL,H1975 CELL,H1299 CELL,SPCA1 CELL AND HBE CELL, DILUTION:NA

He L,Wang Z,Zhou R,Xiong W,Yang Y,Song N,Qian J. Dexmedetomidine exerts cardioprotective effect through miR-146a-3p targeting IRAK1 and TRAF6 via inhibition of the NF-κB pathway. Biomed Pharmacother.2021 Jan;133:110993.doi:10.1016/j.biopha.2020.110993.Epu
Species: Rat
M00227-1 usage in article: APP:WB, SAMPLE:H9C2 CELL, DILUTION:1:1000

ANXA2 silencing inhibits proliferation, invasion, and migration in gastric cancer cells Rui Xie 1, et al. J Oncol. 2019 May 2;2019:4035460. doi: 10.1155/2019/4035460. eCollection 2019.

MiR%u201030a%u20105p ameliorates spinal cord injury%u2010induced inflammatory responses and oxidative stress by targeting Neurod 1 through MAPK/ERK signalling

Hypoxia inducible factor 1%u03B1 promotes survival of mesenchymal stem cells under hypoxia

Astragaloside IV inhibits ventricular remodeling and improves fatty acid utilization in rats with chronic heart failure

The relationship between bile acid concentration, glucagon-like-peptide 1, fibroblast growth factor 15 and bile acid receptors in rats during progression of %u2026

Puerarin protects rat brain against ischemia/reperfusion injury by suppressing autophagy via the AMPK-mTOR-ULK1 signaling pathway

Research on Roles of Mongolian Medical Warm Acupuncture in Inhibiting p38 MAPK Activation and Apoptosis of Nucleus Pulposus Cells

Glucagon-like peptide-1 receptor agonists inhibit hepatic stellate cell activation by blocking the p38 MAPK signaling pathway

Orally administered berberine modulates hepatic lipid metabolism by altering microbial bile acid metabolism and the intestinal FXR signaling pathway

Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter

Alterations in necroptosis during ALDH2%u2011mediated protection against high glucose%u2011induced H9c2 cardiac cell injury

WNT10A/%u03B2%u2011catenin pathway in tumorigenesis of papillary thyroid carcinoma

microRNA-130a is an oncomir suppressing the expression of CRMP4 in gastric cancer

Age-dependent down-regulation of hyperpolarization-activated cyclic nucleotide-gated channel 4 causes deterioration of canine sinoatrial node function

The legacy effects of electromagnetic fields on bone marrow mesenchymal stem cell self-renewal and multiple differentiation potential

Polydatin attenuates diet-induced nonalcoholic steatohepatitis and fibrosis in mice

CXCL8 Antagonist Improves Diabetic Nephropathy in Male Mice With Diabetes and Attenuates High Glucose%u2013Induced Mesangial Injury

KPNA2 promotes metabolic reprogramming in glioblastomas by regulation of c-myc

Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC)

The putative tumour suppressor miR-1-3p modulates prostate cancer cell aggressiveness by repressing E2F5 and PFTK1

Dicer regulates non-homologous end joining and is associated with chemosensitivity in colon cancer patients

Expression of Hsp70 reveals significant differences between fin regeneration and inflammation in Paramisgurnus dabryanus

Rs12976445 Polymorphism Is Associated with Post-Ablation Recurrence of Atrial Fibrillation by Modulating the Expression of MicroRNA-125a and Interleukin %u2026

High levels of glucose promote the activation of hepatic stellate cells via the p38-mitogen-activated protein kinase signal pathway

Synergistic effect of TRAIL and irradiation in elimination of glioblastoma stem-like cells

The notch pathway promotes NF-%u03BAB activation through Asb2 in T cell acute lymphoblastic leukemia cells

HDAC6 inhibition suppresses chondrosarcoma by restoring the expression of primary cilia

Radiation-induced G2/M arrest rarely occurred in glioblastoma stem-like cells

Wear particles enhance autophagy through up-regulation of CD147 to promote osteoclastogenesis

Phenylephrine promotes cardiac fibroblast proliferation through calcineurin-NFAT pathway

Kaempferol inhibits interleukin%u20111%u03B2 stimulated matrix metalloproteinases by suppressing the MAPK%u2011associated ERK and P38 signaling pathways

DYT-40, a novel synthetic 2-styryl-5-nitroimidazole derivative, blocks malignant glioblastoma growth and invasion by inhibiting AEG-1 and NF-?B signaling pathways

Inhibition of STAT1 sensitizes radioresistant nasopharyngeal carcinoma cell line CNE-2R to radiotherapy

NDRG4 promotes myogenesis via Akt/CREB activation

Alleviation of Toxicity Caused by Overactivation of Ppar? through Ppar?-Inducible miR-181a2

The role of copper transporter ATP7A in platinum-resistance of esophageal squamous cell cancer (ESCC)

Combined effects of interleukin-1? and cyclic stretching on metalloproteinase expression in corneal fibroblasts in vitro

Regulation of tumorigenesis in oral epithelial cells by defined reprogramming factors Oct4 and Sox2

miR-506 inhibits cell proliferation and invasion by targeting TET family in colorectal cancer

Knockdown of versicanV1 induces a severe inflammatory response in LPS-induced acute lung injury via the TLR2-NF-?B signaling pathway in C57BL/6J mice

Iron-Mediated Lysosomal Membrane Permeabilization in Ethanol-Induced Hepatic Oxidative Damage and Apoptosis: Protective Effects of Quercetin

Targeted p53 activation by saRNA suppresses human bladder cancer cells growth and metastasis

Targeting the D1-N-methyl-D-aspartate receptor complex reduces L-dopa-induced dyskinesia in 6-hydroxydopamine-lesioned Parkinson's rats

Involvement of epithelial-to-mesenchymal transition and associated transforming growth factor-?/Smad signaling in paraquat-induced pulmonary fibrosis

SIRT1 protects against myocardial ischemia?reperfusion injury via activating eNOS in diabetic rats

Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD

IL-10 Protects Neurites in Oxygen-Glucose-Deprived Cortical Neurons through the PI3K/Akt Pathway

Dasatinib suppresses invasion and induces apoptosis in nasopharyngeal carcinoma

TSPAN8 promotes gastric cancer growth and metastasis via ERK MAPK pathway

Endogenous Nodal promotes melanoma undergoing epithelial-mesenchymal transition via Snail and Slug in vitro and in vivo.

Clinicopathologic and prognostic significance of p21 (Cip1/Waf1) expression in bladder cancer.

EGFR tyrosine kinase inhibitors promote pro-caspase-8 dimerization that sensitizes cancer cells to DNA-damaging therapy

Downregulation of phosphoglycerate dehydrogenase inhibits proliferation and enhances cisplatin sensitivity in cervical adenocarcinoma cells by regulating Bcl-2 and caspase-3

High-density lipoprotein induces cyclooxygenase-2 expression and prostaglandin I-2 release in endothelial cells through sphingosine kinase-2

mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27

Cytochrome P450 26A1 modulates natural killer cells in mouse early pregnancy

Acupuncture Alters Expression of Insulin Signaling Related Molecules and Improves Insulin Resistance in OLETF Rats

Microarray-based identification of genes associated with cancer progression and prognosis in hepatocellular carcinoma

Inhibition of SDF-1?/CXCR4 Signalling in Subchondral Bone Attenuates Post-Traumatic Osteoarthritis

The combination of blueberry juice and probiotics reduces apoptosis of alcoholic fatty liver of mice by affecting SIRT1 pathway

Autophagy protects ovarian cancer-associated fibroblasts against oxidative stress

Dual Receptor Recognizing Cell Penetrating Peptide for Selective Targeting, Efficient Intratumoral Diffusion and Synthesized Anti-Glioma Therapy

Glucagon-like peptide-1 protects cardiomyocytes from advanced oxidation protein product-induced apoptosis via the PI3K/Akt/Bad signaling pathway.

Quercetin Alleviates High-Fat Diet-Induced Oxidized Low-Density Lipoprotein Accumulation in the Liver: Implication for Autophagy Regulation

Higher heat shock factor 1 expression in tumor stroma predicts poor prognosis in esophageal squamous cell carcinoma patients

miR-124 and miR-506 inhibit colorectal cancer progression by targeting DNMT3B and DNMT1

Knockdown of Gli1 by small-interfering RNA enhances the effects of BCNU on the proliferation and apoptosis of glioma U251 cells

Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats

Inhibition of DNA methyltransferase as a novel therapeutic strategy to overcome acquired resistance to dual PI3K/mTOR inhibitors

Cai XS, Tan ZG, Li JJ, Gao WH, Li SJ, Li JL, Tang YM, Li HW, Hui HX. Med Sci Monit. 2017 Sep 8;23:4334-4342. Glucagon-Like Peptide-1 (GLP-1) Treatment Ameliorates Cognitive Impairment by Attenuating Arc Expression in Type 2 Diabetic Rats

Li X, Wang MH, Qin C, Fan WH, Tian DS, Liu JL. PLoS One. 2017 Nov 29;12(11):e0188748. doi: 10.1371/journal.pone.0188748. eCollection 2017. Fingolimod suppresses neuronal autophagy through the mTOR/p70S6K pathway and alleviates ischemic brain damag...

Jiang Q, Ma L, Li R, Sun J. Oncotarget. 2017 Dec 1;9(15):11989-11998. doi: 10.18632/oncotarget.22857. eCollection 2018 Feb 23. Colon cancer-induced interleukin-35 inhibits beta-catenin-mediated pro-oncogenic activity

Hu Y, Song J, Liu L, Zhang Y, Wang L, Li Q. Front Cell Infect Microbiol. 2018 Apr 9;8:110. doi: 10.3389/fcimb.2018.00110. eCollection 2018. microRNA-4516 Contributes to Different Functions of Epithelial Permeability Barrier by Targeting Poliovirus...

Wang W, Qing X, Wang B, Ma K, Wei Y, Shao Z. Evid Based Complement Alternat Med. 2018 Mar 12;2018:6719460. doi: 10.1155/2018/6719460. eCollection 2018. Tauroursodeoxycholic Acid Protects Nucleus Pulposus Cells from Compression-Induced Apoptosis an...

Feng X, Lin J, Xing S, Liu W, Zhang G. BMC Cancer. 2017 Jan 31;17(1):90. doi: 10.1186/s12885-017-3068-0. Higher IGFBP-1 to IGF-1 serum ratio predicts unfavourable survival in patients with nasopharyngeal carcinoma

Liao Y, Xing S, Xu B, Liu W, Zhang G. Oncotarget. 2017 May 2;8(18):30050-30062. doi: 10.18632/oncotarget.16274. Evaluation of the circulating level of fibroblast activation protein α for diagnosis of esophageal squamous cell carcinoma

Zheng M, Chen R, Chen H, Zhang Y, Chen J, Lin P, Lan Q, Yuan Q, Lai Y, Jiang X, Pan X, Liu N. Front Cell Neurosci. 2018 Feb 13;12:13. doi: 10.3389/fncel.2018.00013. eCollection 2018. Netrin-1 Promotes Synaptic Formation and Axonal Regeneration via...

Wang C, Ge Q, Chen Z, Hu J, Li F, Song X, Xu H, Ye Z. Biomed Res Int. 2015;2015:304753. Doi: 10.1155/2015/304753. Epub 2015 Mar 30. A New Double Stranded Rna Suppresses Bladder Cancer Development By Upregulating P21 (Waf1/Cip1) Expression.

Wu Q, Chang Y, Zhang L, Zhang Y, Tian T, Feng G, Zhou S, Zheng Q, Han F, Huang F. J Cancer. 2013 Nov 21;4(9):727-35. Doi: 10.7150/Jca.7576. Ecollection 2013. Srpk1 Dissimilarly Impacts On The Growth, Metastasis, Chemosensitivity And Angiogenesis O...

Lu Y, Zhang K, Li C, Yao Y, Tao D, Liu Y, Zhang S, Ma Y. Plos One. 2012;7(1):E30999. Doi: 10.1371/Journal.Pone.0030999. Epub 2012 Jan 27. Piwil2 Suppresses P53 By Inducing Phosphorylation Of Signal Transducer And Activator Of Transcription 3 In Tu...

Meng R, Zhu D, Bi Y, Yang D, Wang Y. Plos One. 2013;8(1):E53557. Doi: 10.1371/Journal.Pone.0053557. Epub 2013 Jan 10. Erythropoietin Inhibits Gluconeogenesis And Inflammation In The Liver And Improves Glucose Intolerance In High-Fat Diet-Fed Mice.

Chen C, Mei H, Shi W, Deng J, Zhang B, Guo T, Wang H, Hu Y. Plos One. 2013 Apr 10;8(4):E60860. Doi: 10.1371/Journal.Pone.0060860. Print 2013. Egfp-Egf1-Conjugated Plga Nanoparticles For Targeted Delivery Of Sirna Into Injured Brain Microvascular E...

Zafar Mi, Hu C, Liu D, Shafqat Ra, Gao F. J Diabetes Res. 2014;2014:458104. Doi: 10.1155/2014/458104. Epub 2014 Aug 14. Insulin Detemir Causes Lesser Weight Gain In Comparison To Insulin Glargine: Role On Hypothalamic Npy And Galanin.

Zhao Y, Xie P, Fan H. Environ Sci Technol. 2012 Jan 3;46(1):34-41. Doi: 10.1021/Es201514H. Epub 2011 Sep 19. Genomic Profiling Of Micrornas And Proteomics Reveals An Early Molecular Alteration Associated With Tumorigenesis Induced By Mc-Lr In Mice.

Li Y, Xu N, Cai L, Gao Z, Shen L, Zhang Q, Hou W, Zhong H, Wang Q, Xiong L. Plos One. 2013;8(2):E57130. Doi: 10.1371/Journal.Pone.0057130. Epub 2013 Feb 22. Ndrg2 Is A Novel P53-Associated Regulator Of Apoptosis In C6-Originated Astrocytes Exposed...

Liu Y, Zhang Y, Lin L, Lin F, Li T, Du H, Chen R, Zheng W, Liu N. Plos One. 2013 Nov 12;8(11):E78514. Doi: 10.1371/Journal.Pone.0078514. Ecollection 2013. Effects Of Bone Marrow-Derived Mesenchymal Stem Cells On The Axonal Outgrowth Through Activa...

Liu M, Wang Q, Liu F, Cheng X, Wu X, Wang H, Wu M, Ma Y, Wang G, Hao H. Plos One. 2013 Nov 14;8(11):E79172. Doi: 10.1371/Journal.Pone.0079172. Ecollection 2013. Udp-Glucuronosyltransferase 1A Compromises Intracellular Accumulation And Anti-Cancer ...

Zhu W, Chen S, Li Z, Zhao X, Li W, Sun Y, Zhang Z, Ling W, Feng X. Nutr Metab (Lond). 2014 Aug 12;11:35. Doi: 10.1186/1743-7075-11-35. Ecollection 2014. Effects And Mechanisms Of Resveratrol On The Amelioration Of Oxidative Stress And Hepatic Stea...

Zhai B, Liu H, Li X, Dai L, Gao Y, Li C, Zhang L, Ding Y, Yu X, Zhang J. Cell Physiol Biochem. 2013;32(2):264-78. Doi: 10.1159/000354435. Epub 2013 Jul 31. Bmp15 Prevents Cumulus Cell Apoptosis Through Ccl2 And Fbn1 In Porcine Ovaries.

Liu F, Yu G, Wang G, Liu H, Wu X, Wang Q, Liu M, Liao K, Wu M, Cheng X, Hao H. Plos One. 2012;7(7):E42138. Doi: 10.1371/Journal.Pone.0042138. Epub 2012 Jul 27. An Nqo1-Initiated And P53-Independent Apoptotic Pathway Determines The Anti-Tumor Effec...

Wu G, Yu F, Xiao Z, Xu K, Xu J, Tang W, Wang J, Song E. Br J Cancer. 2011 Jun 28;105(1):146-53. Doi: 10.1038/Bjc.2011.190. Epub 2011 May 31. Hepatitis B Virus X Protein Downregulates Expression Of The Mir-16 Family In Malignant Hepatocytes In Vitro.

Yu Y, Xie M, He Yl, Xu Wq, Zhu S, Cao Lz. Ai Zheng. 2008 Sep;27(9):929-33. [Role Of High Mobility Group Box 1 In Adriamycin-Induced Apoptosis In Leukemia K562 Cells].

Xu G, Zhang Y, Wei J, Jia W, Ge Z, Zhang Z, Liu X. Bmc Cancer. 2013 Oct 10;13:469. Doi: 10.1186/1471-2407-13-469. Microrna-21 Promotes Hepatocellular Carcinoma Hepg2 Cell Proliferation Through Repression Of Mitogen-Activated Protein Kinase-Kinase 3.

Liu Z, Wang T, Yang J, Wang S, Yang W, Liu J, Ye Z. Mol Med Rep. 2012 May;5(5):1241-4. Doi: 10.3892/Mmr.2012.813. Epub 2012 Feb 28. Calcium Oxalate Monohydrate Crystals Stimulate Monocyte Chemoattractant Protein-1 And Transforming Grow...

Wu Ty, Zhang Th, Qu Lm, Feng Jp, Tian Ll, Zhang Bh, Li Dd, Sun Yn, Liu M. Int J Clin Exp Pathol. 2013 Dec 15;7(1):56-63. Ecollection 2014. Mir-19A Is Correlated With Prognosis And Apoptosis Of Laryngeal Squamous Cell Carcinoma By Regulating Timp-2...

Zhou X, Wu W, Chen J, Wang X, Wang Y. Nutr Metab (Lond). 2015 Mar 8;12:10. Doi: 10.1186/S12986-015-0006-5. Ecollection 2015. Amp-Activated Protein Kinase Is Required For The Anti-Adipogenic Effects Of Alpha-Linolenic Acid.

Zhang S, Xie R, Wan F, Ye F, Guo D, Lei T. Oncol Lett. 2013 Dec;6(6):1649-1655. Epub 2013 Oct 11. Identification Of U251 Glioma Stem Cells And Their Heterogeneous Stem-Like Phenotypes.

Zhang K, Lu Y, Yang P, Li C, Sun H, Tao D, Liu Y, Zhang S, Ma Y. Plos One. 2012;7(7):E41973. Doi: 10.1371/Journal.Pone.0041973. Epub 2012 Jul 27. Hili Inhibits Tgf-?? Signaling By Interacting With Hsp90 And Promoting T??r Degradation.

Huang Gf, Zou J, Shi J, Zhang Dy, Pen Hf, Zhang Q, Gao Y, Wang By. Evid Based Complement Alternat Med. 2014;2014:731395. Doi: 10.1155/2014/731395. Epub 2014 May 27. The Effect Of Electroacupuncture On The Extracellular Matrix Synthesis And Degrada...

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17 Customer Q&As for Anti-GAPDH Rabbit Monoclonal Antibody

Question

Will anti-GAPDH Rabbit Monoclonal antibody M00227-1 work for Flow Cytometry with lymphoblast?

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

According to the expression profile of lymphoblast, GAPDH is highly expressed in lymphoblast. So, it is likely that anti-GAPDH Rabbit Monoclonal antibody M00227-1 will work for Flow Cytometry with lymphoblast.

Boster Scientific Support

Answered: 2020-03-30

Question

I have a question about product M00227-1, anti-GAPDH Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-24

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00227-1 anti-GAPDH Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-24

Question

Is there a BSA free version of anti-GAPDH Rabbit Monoclonal antibody M00227-1 available?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GAPDH Rabbit Monoclonal antibody M00227-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-14

Question

Is a blocking peptide available for product anti-GAPDH Rabbit Monoclonal antibody (M00227-1)?

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

We do provide the blocking peptide for product anti-GAPDH Rabbit Monoclonal antibody (M00227-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-04

Question

Does anti-GAPDH Rabbit Monoclonal antibody M00227-1 work on canine Flow Cytometry with cervix carcinoma?

K. Mitchell

Verified customer

Asked: 2019-07-19

Answer

Our lab technicians have not validated anti-GAPDH Rabbit Monoclonal antibody M00227-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-GAPDH Rabbit Monoclonal antibody M00227-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cervix carcinoma in Flow Cytometry, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-19

Question

We have been able to see staining in rat muscle. Do you have any suggestions? Is anti-GAPDH Rabbit Monoclonal antibody supposed to stain muscle positively?

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

Based on literature muscle does express GAPDH. Based on Uniprot.org, GAPDH is expressed in smooth muscle tissue, liver, lung, placenta, astrocytoma, leukemia, colon adenocarcinoma, eye, kidney, lung, lymph placenta, muscle, platelet, prostatic carcinoma, brain, cajal-retzius cell fetal brain cortex, heart, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305

Boster Scientific Support

Answered: 2019-07-03

Question

My boss were happy with the WB result of your anti-GAPDH Rabbit Monoclonal antibody. However we have observed positive staining in cervix carcinoma erythroleukemia cytosol using this antibody. Is that expected? Could you tell me where is GAPDH supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

From literature, cervix carcinoma erythroleukemia does express GAPDH. Generally GAPDH expresses in cytoplasm, cytosol. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305

Boster Scientific Support

Answered: 2019-06-18

Question

I was wanting to use your anti-GAPDH Rabbit Monoclonal antibody for Flow Cytometry for cow lymphoblast on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for cow lymphoblast identification?

Verified Customer

Verified customer

Asked: 2019-02-11

Answer

You can see on the product datasheet, M00227-1 anti-GAPDH Rabbit Monoclonal antibody has been tested for Flow Cytometry, IF, IHC, ICC, WB on canine, chicken, cow, human, monkey, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in cow lymphoblast in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-02-11

Question

I see that the anti-GAPDH Rabbit Monoclonal antibody M00227-1 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-01-16

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-01-16

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphoblast using anti-GAPDH Rabbit Monoclonal antibody M00227-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-05-30

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-05-30

Question

We are currently using anti-GAPDH Rabbit Monoclonal antibody M00227-1 for chicken tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says canine, chicken, cow, human, monkey, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?

B. Jones

Verified customer

Asked: 2018-04-04

Answer

The anti-GAPDH Rabbit Monoclonal antibody (M00227-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-04-04

Question

We want to test anti-GAPDH Rabbit Monoclonal antibody M00227-1 on cow lymphoblast for research purposes, then I may be interested in using anti-GAPDH Rabbit Monoclonal antibody M00227-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-04-02

Answer

The products we sell, including anti-GAPDH Rabbit Monoclonal antibody M00227-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-04-02

Question

We are interested in using your anti-GAPDH Rabbit Monoclonal antibody for antimicrobial humoral immune response mediated by antimicrobial peptide studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-03-09

Answer

We appreciate your inquiry. This M00227-1 anti-GAPDH Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for Flow Cytometry, IF, IHC, ICC, WB in canine, chicken, cow, human, monkey, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-03-09

Question

See attached the WB image, lot number and protocol we used for lymphoblast using anti-GAPDH Rabbit Monoclonal antibody M00227-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-01-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-05

Question

Will M00227-1 anti-GAPDH Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-09-27

Answer

As indicated on the product datasheet, M00227-1 anti-GAPDH Rabbit Monoclonal antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-09-27

Question

We bought anti-GAPDH Rabbit Monoclonal antibody for IHC on leukemia in the past. I am using canine, and We are going to use the antibody for Flow Cytometry next. I am interested in examining leukemia as well as lymphoblast in our next experiment. Do you have any suggestion on which antibody would work the best for Flow Cytometry?

M. Wu

Verified customer

Asked: 2015-06-17

Answer

I have checked the website and datasheets of our anti-GAPDH Rabbit Monoclonal antibody and I see that M00227-1 has been tested on canine in both IHC and Flow Cytometry. Thus M00227-1 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in canine even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2015-06-17

Question

Is this M00227-1 anti-GAPDH Rabbit Monoclonal antibody reactive to the isotypes of GAPDH?

H. Li

Verified customer

Asked: 2014-09-15

Answer

The immunogen of M00227-1 anti-GAPDH Rabbit Monoclonal antibody is A synthesized peptide derived from human GAPDH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-09-15

Size

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M00227-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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