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Product Info Summary
| SKU: | A00227-HRP |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Chicken, Human, Monkey, Mouse, Rat, Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GAPDH Antibody Picoband® (HRP)
SKU/Catalog Number
A00227-HRP
Size
100μg/vial
Form
Liquid
Description
Boster Bio Anti-GAPDH Antibody (HRP) (Catalog # A00227-HRP). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
HRP-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-GAPDH Antibody Picoband® (HRP) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00227-HRP)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.
Cross-reactivity
HRP-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.
Reactive Species
A00227-HRP is reactive to GAPDH in Chicken, Human, Monkey, Mouse, Rat, Zebrafish
Observed Molecular Weight
36 kDa
Calculated molecular weight
36.1 kDa
Background of GAPDH
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00227-HRP is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.02-0.1 μg/ml
Positive Control
WB: human Hela whole cell, human A549 whole cell, human A562 whole cell, human MCF-7 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue, chicken heart tissue, chicken liver tissue, chicken brain tissue, monkey heart tissue, monkey lung tissue, monkey kidney tissue, monkey liver tissue
Validation Images & Assay Conditions
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Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-HRP).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human A562 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (Catalog # A00227-HRP) at 0.1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
Click image to see more details
Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-HRP).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: chicken heart tissue lysates,
Lane 2: chicken liver tissue lysates,
Lane 3: chicken brain tissue lysates,
Lane 4: monkey heart tissue lysates,
Lane 5: monkey lung tissue lysates,
Lane 6: monkey kidney tissue lysates,
Lane 7: monkey liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (Catalog # A00227-HRP) at 0.1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
Specific Publications For Anti-GAPDH Antibody Picoband® (HRP) (A00227-HRP)
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