Product Info Summary
SKU: | M01263 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-beta Actin ACTB Rabbit Monoclonal Antibody
View all beta-Actin Antibodies
SKU/Catalog Number
M01263
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-beta Actin ACTB Rabbit Monoclonal Antibody catalog # M01263. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta Actin ACTB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01263)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BF-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human beta Actin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01263 is reactive to ACTB in Human, Mouse, Rat
Applications
M01263 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
41.737kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:50000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of beta Actin expression in (1)HeLa cell lysate; (2)Human fetal kidney lysate; (3) NIH/3T3 cell lysate; (4) PC-12 cell lysate.
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All lanes use beta Actin Antibody at 1:40000 dilution for 1 hour at room temperature.
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All lanes use beta Actin Antibody at 1:50000 dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded (1) Human heart; (2) Mouse testis; (3) Human bladder cancer; (4) Mouse heart, using beta Actin Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For ACTB (Source: Uniprot.org, NCBI)
Gene Name
ACTB
Full Name
Actin, cytoplasmic 1
Weight
41.737kDa
Superfamily
actin family
Alternative Names
ACTB; actin, beta; actin, cytoplasmic 1; beta actin; B-Actin; beta actin (ac-15); Beta Actin; beta cytoskeletal actin; beta-actin ac 15; beta-actin ac15; beta-actin ac-15; betaActin; beta-Actin; BRWS1; I(2)-Actin; PS1TP5-binding protein 1; PS1TP5BP1 ACTB BRWS1, PS1TP5BP1 actin beta actin, cytoplasmic 1|I(2)-actin|PS1TP5-binding protein 1|beta cytoskeletal actin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTB, check out the ACTB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta Actin ACTB Rabbit Monoclonal Antibody (M01263)
Hello CJ!
M01263 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of endoplasmic reticulum stress involved in manganese‑induced neurotoxicity in rats
Zileuton ameliorates depressive-like behaviors, hippocampal neuroinflammation, apoptosis and synapse dysfunction in mice exposed to chronic mild stress
Long non-coding RNA expression profile in Cdk5-knockdown mouse skin
Prolonged overnutrition with fructose or fat induces metabolic derangements in rats by disrupting the crosstalk between the hypothalamus and periphery: Possible amelioration with fenofibrate
Indomethacin decreases insulin secretion by reducing KCa3.1 as a biomarker of pancreatic tumor and causes apoptotic cell death
Liu J,Yang C,Yang J,Song X,Han W,Xie M,Cheng L,Xie L,Chen H,Jiang L.Effects of early postnatal exposure to fine particulate matter on emotional and cognitive development and structural synaptic plasticity in immature and mature rats.Brain Behav.2019 Dec;9(12):e01453.doi:10.1002/brb3.1453.Epub 2019 Nov 11.PMID:31709780; PMCID:PMC6908876.
Species: Rat
M01263 usage in article: APP:WB, SAMPLE:HIPPOCAMPI, DILUTION:1:500
Li Z,Yulei J,Yaqing J,Jinmin Z,Xinyong L,Jing G,Min L.Protective effects of tetramethylpyrazine analogue Z-11 on cerebral ischemia reperfusion injury.Eur J Pharmacol.2019 Feb 5;844:156-164.doi:10.1016/j.ejphar.2018.11.031.Epub 2018 Nov 29.PMID:30502344.
Species: Mouse,Rat
M01263 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:NA
Yang H,Lu Z,Huo C,Chen Y,Cao H,Xie P,Zhou H,Liu D,Liu J,Yu L.Liang-Ge-San, a Classic Traditional Chinese Medicine Formula, Attenuates Lipopolysaccharide-Induced Acute Lung Injury Through Up-Regulating miR-21.Front Pharmacol.2019 Nov 14;10:1332.doi:10.3389/fphar.2019.01332.PMID:31803051;PMCID:PMC6868520.
Species: Mouse
M01263 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA
Xu Y,Wang P,Xu C,Shan X,Feng Q.Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression.J Biomed Res.2019 Jun 4;33(3):181-191.doi:10.7555/JBR.31.20170016.PMID:28963442;PMCID:PMC6551424.
Species: Human
M01263 usage in article: APP:WB, SAMPLE:HEPG2 CELL, DILUTION:NA
Chen B,Li X,Sun Y,Hou Y,Shen J,Tong C,Ma J.Study of the effects of rabbit scleral fibroblasts on cellular biomechanical properties and MMP-2 expression using two modes of riboflavin/ultraviolet A wave collagen cross-linking.Exp Eye Res.2021 Jul 3:108695.doi:10.1016/j.exer.2021.108695.Epub ahead of print.PMID:34228966.
Species: New Zealand White Rabbit
M01263 usage in article: APP:WB, SAMPLE:FIBROBLASTS, DILUTION:1:100
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