Skip to the beginning of the images gallery

ACTB antibody (M01263) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 254 Publications

Datasheet MSDS Request COA

View All Images (9)

Immunofluorescent analysis using the Antibody at 1:50 dilution.

Bundle Save

Bundle & Save Program

Save up to 50% on Picoband® antibodies + earn 10% back on eligible partner reagents.

View Details

BUY MORE, SAVE MORE

This antibody qualifies!
2 = 20% off · 3 = 30% off · 4 = 40% off · 5+ = 50% off

View Details

Free Secondary Antibody

How to redeem? While adding to cart, choose a size with a FREE Secondary Antibody!

View Details

Anti-beta Actin ACTB Rabbit Monoclonal Antibody

ACTB antibody

254 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IF, IHC, ICC, WB

Tested Applications

ACTB antibody was positively detected by WB in:

human GES-1 whole cell, human Hela whole cell, human A431 whole cell, human K562 whole cell, rat C6 whole cell, rat PC-12 whole cell, mouse Neuro-2a whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

ACTB antibody was positively detected by IHC in:

Human heart, Mouse testis, Human bladder cancer, Mouse heart tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ICC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-beta Actin ACTB Rabbit Monoclonal Antibody
View all ACTB Antibodies

Form

Liquid

Uniprot

P60709

Gene ID

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

BF-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human beta Actin

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

42 kDa

Calculated Molecular Weight

41.7 kDa

Cite This Product

Anti-beta Actin ACTB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01263)

Protocols

Western Blot protocol for ACTB antibody

Review Protocol

Immunohistochemistry (IHC) protocol for ACTB antibody

Review Protocol

Publications

Protocol for studying Chemerin 15-enhanced microglial phagocytosis in cerebral ischemia-reperfusion injury via the ChemR23/p38 MAPK pathway

Published: 2026-07-23

Journal: STAR Protocols

Application: WB

Impact Factor: 1.40

Species: Mouse

PMID: 42490223

Bundle Save

Bundle & Save Program

BUY MORE, SAVE MORE

Free Secondary Antibody

Anti-beta Actin ACTB Rabbit Monoclonal Antibody

ACTB antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Protocol for studying Chemerin 15-enhanced microglial phagocytosis in cerebral ischemia-reperfusion injury via the ChemR23/p38 MAPK pathway

Atractylenolide Ⅲ protects granulosa cells from oxidative injury by regulating the NOX2/NRF2 axis in polycystic ovary syndrome

LncRNA ANRIL inhibits TGF-β1-induced proliferation, migration, and fibrosis of Tenon’s capsule fibroblasts from glaucoma patients by reducing IL-22-RA1 stability and expression

Tumor-induced reprogramming of microglia to a DAM phenotype facilitates brain metastasis

MYLIP-dependent ubiquitination and degradation of LDLR in acute myeloid leukemia and MAPK signaling

Design, synthesis, and activity study of heterocyclic derivatives as JAK inhibitors

Study on the percutaneous absorption and synergistic anti-rheumatoid arthritis effect of the Zanthoxylum bungeanum essential oil on Qin Jiao-Du Huo

Effect of berberine-loaded ultrasonic micro-arc oxidation coating on the antibacterial performance and cytocompatibility of biomedical magnesium alloys

Wt1 facilitates visceral beige fat formation to combat abdominal obesity

Ovary single-nucleus transcriptomics reveal follicle cell maturation as a driver of reproductive hierarchy in bumblebees

Tanshinone IIA, salvianic acid A, salvianolic acid B, and hydroxysafflor yellow A reduce cerebral injury via TGF-β1/Smad3 pathway

Crocin Ameliorates Testicular Damage in Type 1 Diabetic Rats: Role of Akt-Mediated Nrf-2 Activation

Modulation of vascular remodeling and vasoconstriction by α-phellandrene in hypoxia-induced pulmonary hypertension

Exosomes Derived From Mesenchymal Stem Cells Regulating Myotube Cell Atrophy via NF‐κB Signaling Pathway

The Radioprotective Effects of (−)-Epigallocatechin-3-gallate (EGCG): Restoring the Gut Microbiota-Immune Axis to Ameliorate Radiation-Induced Intestinal Injury

The multilayer coatings on polylactic acid implants spatiotemporally regulates the microenvironment to enhance antibacterial and osseointegration capacity

Targeting the 4-HNE–H₂S pathway as a therapeutic strategy for placental injury in pregnancy with MAFLD

Parthenolide Alleviates Peritoneal Fibrosis by Blocking Smad2/3 Phosphorylation via Smad Anchor for Receptor Activation

Yangjing Shugan decoction targets the Sirt1/Nrf2 antioxidant pathway and gut microbiota-metabolite axis to protect against premature ovarian failure

Schisanhenol Inhibits MPTP/MPP+-Induced Ferroptosis in Dopaminergic Neurons Via Nrf2/TrxR1/GPX4 Pathway against Parkinson’s Disease

Amelioration of MASLD via Umbelliferone through Modulation of Hepatic Metabolic Function and Restoration of Gut-Liver Axis Homeostasis

β-Caryophyllene confers protection against type 2 diabetic osteoporosis by blocking ferroptosis via the AMPK/Nrf2 pathway

Gut microbiota dysbiosis impairs TGF-β/Smad4 signaling to drive postoperative metastasis in colorectal cancer

POSTN Enhances the Proliferation of Goat Skin Fibroblasts Through the TGFβ Pathway Activation

BMSC-EVs improve post-stroke cognition by promoting regionally distinct synaptic repair via Sema3G-Nrp2/PlexinA4 Signaling

Caffeine restricts hepatitis B virus transcription by inhibiting γ-H2AX formation

Roumudan decoction attenuates hepatic fibrosis by suppressing the Warburg effect via inhibiting key glycolytic proteins

Early α-Synuclein Pathology Induces Neuroinflammation and Decreases Topoisomerase IIβ Expression in A53T Mice

Investigation of Kai-Xin-San in alleviating cognitive impairment in aβ transgenic Caenorhabditis elegans through mitochondrial function regulation

Morphological and histopathological study of edible bird’s nest on wound healing in mice

Crocetin Attenuates Fibroblast-to-Myofibroblast Transition after Myocardial Infarction in Mice by Regulating Autophagy

Chemerin 15 enhances microglial phagocytosis to attenuate cerebral ischemia-reperfusion injury through the ChemR23/p38 MAPK pathway

Senkyunolide I targets CXCR4 to attenuate liver fibrosis via suppression of the NLRP3/GSDMD pathway

Anti-hyperlipidemic effects of Apocynum venetum L. leaves extract on high-fat diet-induced hyperlipidemia: Modulation of lipid metabolism and oxidative stress

Network pharmacology to explore the novel anti-inflammatory mechanism of naringenin in intestinal ischemia/reperfusion injury

Chrysin inhibits PEDV replication by antagonizing apoptosis via the ROS/JNK/p53 axis

Sinapic acid and glabridin synergistically mitigate androgenetic alopecia by modulating ferroptosis through stabilization of β-catenin against ubiquitin-dependent degradation

Inhibition of Rac1 Attenuated Propofol-Induced Neurotoxicity in the Hippocampal Dentate Gyrus in Developing Mice

Paeonol mitigates chronic stress–induced amygdalar neuronal damage through glycogen synthase kinase-3β/calcineurin axis regulation of synaptic plasticity

Therapeutic Potential of Targeting PCLAF in Endometrial Cancer: Insights from Wnt/β-catenin and p53 Regulatory Mechanisms

MiR-134-5p/BDNF/TrkB/CREB signaling pathway involved in the depression-like behaviors in mice following exposure to benzo[a]pyrene

Insufficient BK channel function enhances NF-κB nuclear translocation and promotes IL-6 synthesis in vascular smooth muscle cells induced by AT1-AA

SMPD3 Inhibition Contributes to Nicotinamide-Ameliorated Hepatic Steatosis in Chronic Alcohol-Fed Mice

Wogonin Attenuates Atherosclerosis via KLF11-Mediated Suppression of PPARα-YAP1-Driven Glycolysis and Enhancement of ABCA1/G1-Mediated Cholesterol Efflux

Ultrasound Visualization of Spatiotemporal Autophagy-Regulated Nanodroplets for Amplifying ICB in Melanoma via Remodeling Tumor Inflammatory Microenvironment

Lnc MSTRG 4701.7 targets miR-1786/RORa to competitively regulate proliferation and apoptosis in chicken follicular granulosa cells

High-dose Vitamin C inhibits PD-L1 expression by activating AMPK in colorectal cancer

1,25-dihydroxyvitamin D3 improves non-alcoholic steatohepatitis phenotype in a diet-induced rat model

Roquin-1 interaction with Regnase-1 inhibits the progression of rheumatoid arthritis via suppressing FGF2 expression and NF-κB pathway

L1CAM mimetic compound duloxetine improves cognitive impairment in 5xFAD mice and protects Aβ1-42-damaged HT22 cells

Folic acid intervention ameliorates hepatic steatosis after long-term alcohol exposure by alleviating endoplasmic reticulum stress

Phosducin inhibits the cell proliferation and promotes the antitumor effect of temozolomide in glioma

Dedifferentiated fat cells-derived exosomes (DFATs-Exos) loaded in GelMA accelerated diabetic wound healing through Wnt/β-catenin pathway

Phytochemical Composition and Skin-Friendly Activities of the Ethyl Acetate Fraction in Ophioglossum vulgatum Linn., an In Vitro Study

Vanillic acid ameliorates collagen-induced arthritis by suppressing the inflammation response via inhibition of the MAPK and NF-κB signaling pathways

Manshenkang granules alleviate fibrosis in chronic renal failure rats by regulating the PDE4b/cAMP pathway

Serine starvation suppresses the progression of esophageal cancer by regulating the synthesis of purine nucleotides and NADPH

Effect of Tristetraprolin on Esophageal Squamous Cell Carcinoma Cell Proliferation

Identification of m6A methyltransferase-related WTAP and ZC3H13 predicts immune infiltrates in glioblastoma

Temporal and spatial expression of Phosphodiesterase-4B after sciatic nerve compression in rats and its mechanism of action on sciatic nerve repair

O-GlcNAcylation attenuates ischemia-reperfusion-induced pulmonary epithelial cell ferroptosis via the Nrf2/G6PDH pathway

Rod-Shaped Microgel Scaffolds with Interconnective Pores and Oxygen-generating Functions Promote Skin Wound Healing and Alleviate Hypertrophic Scar Formation

Antitumor Effects of Tryptanthrin on Colorectal Cancer by Regulating the Mitogen-Activated Protein Kinase Signaling Pathway and Targeting Topo I and IDO1

Opposite effects of sevoflurane at high and low concentration on the airway inflammation in juvenile asthma rats: A TMT-based proteomics analysis study

Pathophysiology-Directed Engineering of a Combination Nanoanalgesic for Neuropathic Pain

Lactobacillus reuteri Assists Engineered Bacteria That Target Tumors to Release PD-L1nb to Mitigate the Adverse Effects of Breast Cancer Immunotherapy

Perillaldehyde pretreatment alleviates cerebral ischemia-reperfusion injury by improving mitochondrial structure and function via the Nrf2/Keap1/Trx2 axis