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Product Info Summary
| SKU: | M00227 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GAPDH Rabbit Monoclonal Antibody
SKU/Catalog Number
M00227
BM3875 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GAPDH Rabbit Monoclonal Antibody catalog # M00227. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAPDH Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00227)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BH-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GAPDH
Reactive Species
M00227 is reactive to GAPDH in Human, Mouse, Rat
Observed Molecular Weight
36 kDa
Calculated molecular weight
36.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00227 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:50000
IHC 1:100-1:500
ICC/IF 1:100-1:250
IP 1:50
FC 1:50
Positive Control
IHC: Rat colon, Mouse testis, Human uterus, Human kidney tissue, Human pituitary adenoma tissue, Human squamous carcinoma tissue, Human testis cancer tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of GAPDH expression in (1) Hela cell lysate; (2)Jurkat cell lysate; (3)Mouse kidney lysate; (4) Mouse spleen lysate; (5) RAW 264.7 cell lysate; (6) Rat brain lysate with GAPDH Antibody.
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All lanes use GAPDH Antibody at 1:50000 dilution for 1 hour at room temperature.
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Effects of n-6 PUFA on liver lipid peroxidation and the inflammatory marker NF-κB in rats with NASH induced by a choline-deficient diet. (A) Liver MDA levels, (B) PPAR-α mRNA expression in the liver. Data are expressed as mean ±SEM; n = 6/group. (C) NF-κB protein expression (~65 kDa) in the liver as analyzed by Western blotting, normalized to GAPDH, with a representative blot (left) and quantification (right). Protein molecular weight standards (kDa) are labeled on the left of each blot. Data are expressed as mean ± SEM; n = 6/group.
Index in PubMed under a CC BY license. PMID: 40626231
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Effects of n-6 PUFA on liver macrophage phenotype in rats with NASH induced by a choline-deficient diet. (A) M1-type Kupffer cells (KCs) identified by double staining: red arrows show CD11c-positive cells, green arrows show CD68-positive cells, and yellow arrows highlight CD11c and CD68 double-positive M1-type KCs (Scale bar – 50 μM). (B) M2-type KCs identified similarly, with red arrows indicating CD163-positive cells, green arrows showing CD68-positive cells, and yellow arrows marking CD163 and CD68 double-positive M2-type KCs (Scale bar – 50 μM). For (A,B) (see ) for full-size photomicrographs. (C) M1/M2 phenotype ratio (unitless), calculated as the proportion of CD68 + CD11c + to CD68 + CD163 + cells. (D) Relative PPAR-γ2 mRNA expression (fold change normalized to GAPDH) in the liver, which is linked to macrophage polarization and inflammation. Data are expressed as mean ±SEM; n = 6/group.
Index in PubMed under a CC BY license. PMID: 40626231
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All lanes use GAPDH Antibody at 1:50000 dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded (1) Rat colon; (2) Mouse testis; (3) Human uterus; (4) Human kidney, using GAPDH Antibody .
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Immunohistochemical analysis of paraffin-embedded Human pituitary adenoma, using the Antibody at 1:400 dilution.
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Immunohistochemical analysis of paraffin-embedded Human squamous carcinoma, using the Antibody at 1:400 dilution.
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Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody at 1:400 dilution.
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Immunofluorescent analysis of Hela cells, using GAPDH Antibody .
Specific Publications For Anti-GAPDH Rabbit Monoclonal Antibody (M00227)
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16 Customer Q&As for Anti-GAPDH Rabbit Monoclonal Antibody
Question
We have tried in the past anti-GAPDH Rabbit Monoclonal antibody for Flow Cytometry on lymph placenta last year. I am using mouse, and We are going to use the antibody for WB next. you antibody examining lymph placenta as well as cervix carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
I took a look at the website and datasheets of our anti-GAPDH Rabbit Monoclonal antibody and it seems that M00227 has been tested on mouse in both Flow Cytometry and WB. Thus M00227 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-03
Question
I was wanting to use your anti-GAPDH Rabbit Monoclonal antibody for Flow Cytometry for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
As indicated on the product datasheet, M00227 anti-GAPDH Rabbit Monoclonal antibody has been tested for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-29
Question
Our team were happy with the WB result of your anti-GAPDH Rabbit Monoclonal antibody. However we have seen positive staining in kidney cytoplasm using this antibody. Is that expected? Could you tell me where is GAPDH supposed to be expressed?
K. Krishna
Verified customer
Asked: 2019-11-06
Answer
Based on literature, kidney does express GAPDH. Generally GAPDH expresses in cytoplasm, cytosol. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305
Boster Scientific Support
Answered: 2019-11-06
Question
Can you help my question with product M00227, anti-GAPDH Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00227 anti-GAPDH Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-16
Question
Is this M00227 anti-GAPDH Rabbit Monoclonal antibody reactive to the isotypes of GAPDH?
Verified Customer
Verified customer
Asked: 2018-07-27
Answer
The immunogen of M00227 anti-GAPDH Rabbit Monoclonal antibody is A synthesized peptide derived from human GAPDH . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-27
Question
I see that the anti-GAPDH Rabbit Monoclonal antibody M00227 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-06-08
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-06-08
Question
We are interested in to test anti-GAPDH Rabbit Monoclonal antibody M00227 on human leukemic t-cell for research purposes, then I may be interested in using anti-GAPDH Rabbit Monoclonal antibody M00227 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-14
Answer
The products we sell, including anti-GAPDH Rabbit Monoclonal antibody M00227, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-14
Question
Would M00227 anti-GAPDH Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-02
Answer
It shows on the product datasheet, M00227 anti-GAPDH Rabbit Monoclonal antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-02
Question
Is there a BSA free version of anti-GAPDH Rabbit Monoclonal antibody M00227 available?
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-GAPDH Rabbit Monoclonal antibody M00227 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-01
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-GAPDH Rabbit Monoclonal antibody M00227. Let me know if you need anything else.
S. Anderson
Verified customer
Asked: 2017-07-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-04
Question
We have seen staining in rat platelet. What should we do? Is anti-GAPDH Rabbit Monoclonal antibody supposed to stain platelet positively?
E. Mitchell
Verified customer
Asked: 2016-09-27
Answer
According to literature platelet does express GAPDH. According to Uniprot.org, GAPDH is expressed in smooth muscle tissue, liver, lung, placenta, astrocytoma, leukemia, colon adenocarcinoma, eye, kidney, lung, lymph placenta, muscle, platelet, prostatic carcinoma, brain, cajal-retzius cell fetal brain cortex, heart, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have GAPDH expression, here are a few articles citing expression in various tissues:
Astrocytoma, Pubmed ID: 10944468
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 7498159
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 2987855, 24275569
Lung, Pubmed ID: 3664468
Lymphoblast, Pubmed ID: 14654843
Muscle, Pubmed ID: 1193541, 11724794
Placenta, Pubmed ID: 1924305
Boster Scientific Support
Answered: 2016-09-27
Question
My question regards using your anti-GAPDH Rabbit Monoclonal antibody for cellular response to interferon-gamma studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
B. Banerjee
Verified customer
Asked: 2016-01-27
Answer
We appreciate your inquiry. This M00227 anti-GAPDH Rabbit Monoclonal antibody is tested on hela cells, mouse kidney. It is guaranteed to work for Flow Cytometry, IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-01-27
Question
Is a blocking peptide available for product anti-GAPDH Rabbit Monoclonal antibody (M00227)?
M. Bhatt
Verified customer
Asked: 2015-04-29
Answer
We do provide the blocking peptide for product anti-GAPDH Rabbit Monoclonal antibody (M00227). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-04-29
Question
See attached the WB image, lot number and protocol we used for leukemic t-cell using anti-GAPDH Rabbit Monoclonal antibody M00227. Please let me know if you require anything else.
B. Kulkarni
Verified customer
Asked: 2015-01-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-21
Question
We are currently using anti-GAPDH Rabbit Monoclonal antibody M00227 for mouse tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
W. Krishna
Verified customer
Asked: 2015-01-20
Answer
The anti-GAPDH Rabbit Monoclonal antibody (M00227) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-20
Question
Does anti-GAPDH Rabbit Monoclonal antibody M00227 work for Flow Cytometry with leukemic t-cell?
J. Anderson
Verified customer
Asked: 2013-02-13
Answer
According to the expression profile of leukemic t-cell, GAPDH is highly expressed in leukemic t-cell. So, it is likely that anti-GAPDH Rabbit Monoclonal antibody M00227 will work for Flow Cytometry with leukemic t-cell.
Boster Scientific Support
Answered: 2013-02-13
