Anti-GFAP Antibody

Boster Bio Anti-GFAP Antibody catalog # PA1239. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 111 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: PA1239
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, WB

Product Name

Anti-GFAP Antibody

See all GFAP products

SKU/Catalog Number

PA1239

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GFAP Antibody catalog # PA1239. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GFAP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1239)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GFAP (417-432aa DGEVIKESKQEHKDVM), identical to the related rat sequence, and different from the related mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1239 is reactive to GFAP in Human, Mouse, Rat

Applications

PA1239 is guaranteed for IF, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunofluorescence, 2μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For GFAP (Source: Uniprot.Org, NCBI)

Uniprot ID

P14136

Gene ID

2670

Gene Name

GFAP

Full Name

Glial fibrillary acidic protein

Weight

49.88kDa

Superfamily

intermediate filament family

Alternative Names

FLJ45472; GFAP astrocytes; GFAP immunohistochemistry; GFAP mouse; GFAP rabbit; GFAP stain; GFAP; glial fibrillary acidic protein GFAP ALXDRD glial fibrillary acidic protein glial fibrillary acidic protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GFAP, check out the GFAP Infographic

GFAP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GFAP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1239 has been cited in 111 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

OLIG2 Drives Abnormal Neurodevelopmental Phenotypes in Human iPSC-Based Organoid and Chimeric Mouse Models of Down Syndrome
Species: Mouse

Xiang Z,Jiang X,Ji R,Yuan H.Enhanced expression of P2X4 purinoceptors in pyramidal neurons of the rat hippocampal CA1 region may be involved ischemia-reperfusion injury.Purinergic Signal.2021 May 9.doi:10.1007/s11302-021-09780-z.Epub ahead of print.PMID:33966147.
Species: Rat
PA1239 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:400

Xiang S,Zhao D,Hao H,Wang XU,Li L,Yang T.α-Helical protein absorption at post-traumatic epileptic foci monitored by Fourier transform infrared mapping.J Biosci.2020;45:55.PMID:32345781.
Species: Rat
PA1239 usage in article: APP:IHC, SAMPLE:FRONTAL BRAIN LOBE, DILUTION:1:100

Zhang Y,Zhang M,Zhu W,Pan X,Wang Q,Gao X,Wang C,Zhang X,Liu Y,Li S,Sun H.Role of Elevated Thrombospondin-1 in Kainic Acid-Induced Status Epilepticus.Neurosci Bull.2020 Mar;36(3):263-276.doi:10.1007/s12264-019-00437-x.Epub 2019 Oct 29.PMID:31664678;PMCID:P
Species: Rat
PA1239 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS AND CORTEX, DILUTION:1:1000

Chen X,Zhang L,Hua F,Zhuang Y,Liu H,Wang S.EphA4 Obstructs Spinal Cord Neuron Regeneration by Promoting Excessive Activation of Astrocytes.Cell Mol Neurobiol.2021 Feb 17.doi:10.1007/s10571-021-01046-x.Epub ahead of print.PMID:33595805.
Species: Mouse
PA1239 usage in article: APP:IF, SAMPLE:SPINAL CORD TISSUE, DILUTION:1:500

Zhang LY,Jin QQ,Hölscher C,Li L.Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model. Neural Regen Res 2021;16:1660-70
Species: Human,Rat
PA1239 usage in article: APP:IF, SAMPLE:SN TISSUE, DILUTION:1:100

Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
PA1239 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Jiangong Wang,Bin Liu,Yong Xu et al. Inhibition of Histamine H3 receptor Attenuates Neuroinflammation and Cognitive Impairments in Alzheimer’s Disease via activating CREB Pathway,16 December 2020, PREPRINT (Version 1) available at Research Square [https:/
Species: Mouse
PA1239 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Ahmed S. Ahmed,JAK-1/STAT-3 pathway mediated role in aging cerebellar cortex degenerative changes of albino wistar rats, Translational Research in Anatomy, 2020,100089,ISSN 2214-854X, https://doi.org/10. 1016/j.tria.2020.100089.
Species: Rat
PA1239 usage in article: APP:IHC, SAMPLE:CEREBELLAR CORTEX, DILUTION:1:20

Zhou Feng,Shengyan Liu,Qianwei Chen,Qiang Tan,Jishu Xian,Hua Feng,Zhi Chen,Gang Li,uPA alleviates kaolin-induced hydrocephalus by promoting the release and activation of hepatocyte growth factor in rats, Neuroscience Letters,Volume 731,2020,135011,ISSN 03
Species: Rat
PA1239 usage in article: APP:IF, SAMPLE:BRAIN TISSUE,

Jian Wang,Xiaolin Li,Yu Song,Qiangfei Su,Xiakeerzhati Xiaohalati,Wen Yang,Luming Xu,Bo Cai,Guobin Wang,Zheng Wang,Lin Wang,Injectable silk sericin scaffolds with programmable shape-memory property and neuro-differentiation-promoting activity for individua
Species: Silkworm,Mouse
PA1239 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:100

Jiangong Wang,Bin Liu,Yong Xu et al. Inhibition of Histamine H3 receptor Attenuates Neuroinflammation and Cognitive Impairments in Alzheimer’s Disease via activating CREB Pathway,16 December 2020,PREPRINT (Version 1)available at Research Square [+https://
Species: Mouse
PA1239 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Luo Z,Wang J,Tang S,Zheng Y,Zhou X,Tian F,Xu Z.Dynamic-related protein 1 inhibitor eases epileptic seizures and can regulate equilibrative nucleoside transporter 1 expression.BMC Neurol.2020 Sep 22;20(1):353.doi:10.1186/s12883-020-01921-y.PMID:32962663;PM
Species: Rat
PA1239 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Wang X,Tong Y,Zhang J,Khan N,Zhang K,Bai H,Zhang Q,Chen Y.Neuroinflammation changes with periodontal inflammation status during periodontitis in wild-type mice.Oral Dis.2020 Aug 20.doi:10.1111/odi.13618.Epub ahead of print.PMID:32815656.
Species: Mouse
PA1239 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:50

Wang X,Zhou W,Li X,Ren J,Ji G,Du J,Tian W,Liu Q,Hao A. Graphene oxide suppresses the growth and malignancy of glioblastoma stem cell-like spheroids via epigenetic mechanisms. J Transl Med.2020 May 14;18(1):200. doi:10.1186/s12967-020-02359-z.PMID:32410622
Species: Human
PA1239 usage in article: APP:WB, SAMPLE:GSCS, DILUTION:1:1000

Li R,Wen Y,Wu B,He M,Zhang P,Zhang Q,Chen Y.MicroRNA-25-3p suppresses epileptiform discharges through inhibiting oxidative stress and apoptosis via targeting OXSR1 in neurons.Biochem Biophys Res Commun. 2020 Mar 19;523(4):859-866.doi:10.1016/j.bbrc.2020.
Species: Rat
PA1239 usage in article: APP:IHC, SAMPLE:HIPPOCAMPUL NEURONS, DILUTION:NA

Deng Z,Wei Y,Yao Y,Gao S,Wang X.Let-7f promotes the differentiation of neural stem cells in rats.Am J Transl Res.2020 Sep 15;12(9):5752-5761.PMID:33042454;PMCID:PMC7540113.
Species: Rat
PA1239 usage in article: APP:IF, SAMPLE:NSCS, DILUTION:1:100

Li X,Shi MQ,Chen C,Du JR.Phthalide derivative CD21 ameliorates ischemic brain injury in a mouse model of global cerebral ischemia: involvement of inhibition of NLRP3.Int Immunopharmacol.2020 Sep;86:106714.doi: 10.1016/j.intimp.2020.106714.Epub 2020 Jun 24
Species: Mouse
PA1239 usage in article: APP:IF, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:100

Xue NY,Ge DY,Dong RJ,Kim HH,Ren XJ,Tu Y.Effect of electroacupuncture on glial fibrillary acidic protein and nerve growth factor in the hippocampus of rats with hyperlipidemia and middle cerebral artery thrombus. Neural Regen Res.2021 Jan;16(1):137-142.doi
Species: Rat
PA1239 usage in article: APP:IHC, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:1:100

Zhu X,Yao Y,Yang J,Zhengxie J,Li X,Hu S,Zhang A,Dong J,Zhang C,Gan G.COX-2-PGE2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice. Int Immunopharmacol. 2020 Oct;87:106801.doi: 10.1016/j.inti
Species: Mouse
PA1239 usage in article: APP:ICC, SAMPLE:BRAIN TISSUE, DILUTION:1:100

Zhou Z,Hou J,Mo Y,Ren M,Yang G,Qu Z,Hu Y.Geniposidic acid ameliorates spatial learning and memory deficits and alleviates neuroinflammation via inhibiting HMGB-1 and downregulating TLR4/2 signaling pathway in APP/PS1 mice.Eur J Pharmacol.2020 Feb 15;869:1
Species: Mouse
PA1239 usage in article: APP:IHC, SAMPLE:BRAIN SECTION, DILUTION:1:200

Wu K,Yue J,Shen K,He J,Zhu G,Liu S,Zhang C,Yang H. Increased Expression of Fibroblast Growth Factor 13 in Cortical Lesions of the Focal Cortical Dysplasia. Brain Res Bull. 2020 Dec 4:S0361-9230(20)30710-3.doi: 10.1016/j.brainresbull.2020.11.023.Epub ahead
Species: Human
PA1239 usage in article: APP:IHC, SAMPLE:BRAIN TISSU E, DILUTION:1:50

Metalloprotease Adam10 suppresses epilepsy through repression of hippocampal neuroinflammation

Activation of LILRB2 signal pathway in temporal lobe epilepsy patients and in a pilocarpine induced epilepsy model

Neuroprotective effects of a GIP analogue in the MPTP Parkinson's disease mouse model

Neuroprotective Effects of Pycnogenol Against Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury in Primary Rat Astrocytes via NF-%u03BAB and ERK1/2 MAPK %u2026

Tumor-associated microglia/macrophages enhance the invasion of glioma stem-like cells via TGF-%u03B21 signaling pathway

Local injection of bone morphogenetic protein 7 promotes neuronal regeneration and motor function recovery after acute spinal cord injury

Electroacupuncture Suppresses the NF-?B Signaling Pathway by Upregulating Cylindromatosis to Alleviate Inflammatory Injury in Cerebral Ischemia/Reperfusion Rats

Interactions between GHRH and GABAARs in the brains of patients with epilepsy and in animal models of epilepsy

Inhibition of P2X7 receptors improves outcomes after traumatic brain injury in rats

Water-soluble ginseng oligosaccharides protect against scopolamine-induced cognitive impairment by functioning as an antineuroinflammatory agent

Reversion of malignant phenotypes of human glioblastoma cells by ?-elemene through ?-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules

The Neuropilin-1 Inhibitor, ATWLPPR Peptide, Prevents Experimental Diabetes-Induced Retinal Injury by Preserving Vascular Integrity and Decreasing Oxidative Stress

Brain Localization and Neurotoxicity Evaluation of Polysorbate 80-Modified Chitosan Nanoparticles in Rats

Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats

Trimebutine, a small molecule mimetic agonist of adhesion molecule L1, contributes to functional recovery after spinal cord injury in mice

Loss of the golgin GM130 causes Golgi disruption, Purkinje neuron loss, and ataxia in mice

In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats

The association and significance of H3K27me3 and a folate metabolic gene ACat2 in neural tube defects

Cyclin-dependent kinase inhibitor flavopiridol promotes remyelination in a cuprizone induced demyelination model

Evaluation of the Combination of Methylprednisolone and Tranilast after Spinal Cord Injury in Rat Models

Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium

Effect of Corilagin on the Proliferation and NF-?B in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells

GDNF Enhances Therapeutic Efficiency of Neural Stem Cells-Based Therapy in Chronic Experimental Allergic Encephalomyelitis in Rat

The hGFAP-driven conditional TSPO knockout is protective in a mouse model of multiple sclerosis

Transplantation of Neural Stem Cells Cotreated with Thyroid Hormone and GDNF Gene Induces Neuroprotection in Rats of Chronic Experimental Allergic Encephalomyelitis

Re-infection of the prion from the scrapie?infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c

Effect of siRNA-Livin on drug resistance to chemotherapy in glioma U251 cells and CD133+ stem cells

Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro

Histidine provides long-term neuroprotection after cerebral ischemia through promoting astrocyte migration

Different methods for inducing adipose-derived stem cells to differentiate into Schwann-like cells

Chronic Noise Exposure Acts Cumulatively to Exacerbate Alzheimer?s Disease-Like Amyloid-? Pathology and Neuroinflammation in the Rat Hippocampus

Acrylamide neurotoxicity on the cerebrum of weaning rats

Optimal electroacupuncture frequency for maintaining astrocyte structural integrity in cerebral ischemia

Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke

Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia

Wang Y, Huang N, Li H, Liu S, Chen X, Yu S, Wu N, Bian XW, Shen HY, Li C, Xiao L. Oncotarget. 2017 Jun 6;8(23):37511-37524. doi: 10.18632/oncotarget.16400. Promoting oligodendroglial-oriented differentiation of glioma stem cell: a repurposing of q...

Zhou J, Wang J, Li W, Wang C, Wu L, Zhang J. Mol Med Rep. 2017 Mar 24. doi: 10.3892/mmr.2017.6371. Paeoniflorin attenuates the neuroinflammatory response in a rat model of chronic constriction injury.

Zhang R, Pei H, Ru L, Li H, Liu G. Biomed Rep. 2013 Nov;1(6):895-900. Epub 2013 Sep 6. Bone Morphogenetic Protein 7 Upregulates The Expression Of Nestin And Glial Fibrillary Acidic Protein In Rats With Cerebral Ischemia-Reperfusion Injury.

Zhang M, Chai Y, Liu T, Xu N, Yang C. Exp Ther Med. 2015 Apr;9(4):1141-1148. Epub 2015 Feb 2. Synergistic Effects Of Buyang Huanwu Decoction And Embryonic Neural Stem Cell Transplantation On The Recovery Of Neurological Function In A Rat Model Of ...

Li T, Mo X, Jiang Z, He W, Lu W, Zhang H, Zhang J, Zeng L, Yang B, Xiao H, Hu Z. Oxid Med Cell Longev. 2012;2012:945071. Doi: 10.1155/2012/945071. Epub 2012 Oct 12. Study Of ??b-Crystallin Expression In Gerbil Bcao Model Of Transient Global Cerebr...

Zhou H, Cheng X, Rao L, Li T, Duan Yy. Acta Biomater. 2013 May;9(5):6439-49. Doi: 10.1016/J.Actbio.2013.01.042. Epub 2013 Feb 9. Poly(3,4-Ethylenedioxythiophene)/Multiwall Carbon Nanotube Composite Coatings For Improving The Stability Of Microelec...

Zhao Bq, Guo Yr, Li Xl, Zang T, Qu Hy, Zhou Jp, Li Q, Sun Mj. Int J Pharm. 2011 Feb 28;405(1-2):1-8. Doi: 10.1016/J.Ijpharm.2010.11.018. Epub 2010 Nov 18. Amelioration Of Dementia Induced By A?? 22-35 Through Rectal Delivery Of Undecapeptide-Hegf ...

Zhang S, Xie R, Wan F, Ye F, Guo D, Lei T. Oncol Lett. 2013 Dec;6(6):1649-1655. Epub 2013 Oct 11. Identification Of U251 Glioma Stem Cells And Their Heterogeneous Stem-Like Phenotypes.

Wang H, Li C, Wang H, Mei F, Liu Z, Shen Hy, Xiao L. Neurosci Bull. 2013 Apr;29(2):251-9. Doi: 10.1007/S12264-013-1323-1. Epub 2013 Apr 5. Cuprizone-Induced Demyelination In Mice: Age-Related Vulnerability And Exploratory Behavior Deficit.

Xu Dh, Yan M, Li Hd, Fang Pf, Liu Yw. Eur J Pharmacol. 2012 Sep 5;690(1-3):68-76. Doi: 10.1016/J.Ejphar.2012.06.032. Epub 2012 Jun 30. Influence Of P-Glycoprotein On Brucine Transport At The In Vitro Blood-Brain Barrier.

Yu Cz, Liu Yp, Liu S, Yan M, Hu Sj, Song Xj. Eur J Pain. 2014 Jan;18(1):76-85. Doi: 10.1002/J.1532-2149.2013.00390.X. Epub 2013 Sep 5. Systematic Administration Of B Vitamins Attenuates Neuropathic Hyperalgesia And Reduces Spinal Neuron Injury Fol...

Bai Y, Xu G, Xu M, Li Q, Qin X. Int J Mol Med. 2014 Dec;34(6):1473-82. Doi: 10.3892/Ijmm.2014.1946. Epub 2014 Sep 24. Inhibition Of Src Phosphorylation Reduces Damage To The Blood-Brain Barrier Following Transient Focal Cerebral Ischemia In Rats.

Zhang L, Wei Ws, Li Yj, Wang Y. Neuropathology. 2011 Apr;31(2):112-21. Doi: 10.1111/J.1440-1789.2010.01137.X. A Rat Model Of Mild Cognitive Impairment Associated With Vascular Factor.

Zhang X, Zheng Z, Shu B, Liu X, Zhang Z, Liu Y, Bai B, Hu Q, Mao P, Wang H. J Virol. 2013 Nov;87(22):12407-21. Doi: 10.1128/Jvi.02090-13. Epub 2013 Sep 11. Human Astrocytic Cells Support Persistent Coxsackievirus B3 Infection.

Huang F, Wang J, Chen A. J Huazhong Univ Sci Technolog Med Sci. 2006;26(2):206-10. Effects Of Co-Grafts Mesenchymal Stem Cells And Nerve Growth Factor Suspension In The Repair Of Spinal Cord Injury.

Ma H, Yu B, Kong L, Zhang Y, Shi Y. Neurochem Res. 2012 Jan;37(1):69-83. Doi: 10.1007/S11064-011-0584-1. Epub 2011 Sep 7. Neural Stem Cells Over-Expressing Brain-Derived Neurotrophic Factor (Bdnf) Stimulate Synaptic Protein Expression And Promote ...

Huo Sj, Li Yc, Xie J, Li Y, Raisman G, Zeng Yx, He Jr, Weng Ch, Yin Zq. Curr Eye Res. 2012 Aug;37(8):749-58. Doi: 10.3109/02713683.2012.697972. Epub 2012 Jun 12. Transplanted Olfactory Ensheathing Cells Reduce Retinal Degeneration In Royal College...

Chen Sw, Chen Yx, Zhang Xr, Qian H, Chen Wz, Xie Wf. Gene Ther. 2008 Nov;15(21):1424-35. Doi: 10.1038/Gt.2008.93. Epub 2008 May 29. Targeted Inhibition Of Platelet-Derived Growth Factor Receptor-Beta Subunit In Hepatic Stellate Cells Ameliorates H...

Hu Ww, Wang Z, Zhang Ss, Jiang L, Zhang J, Zhang X, Lei Qf, Park Hj, Fang Wj, Chen Z. Biomaterials. 2014 Aug;35(26):7386-97. Doi: 10.1016/J.Biomaterials.2014.05.026. Epub 2014 Jun 2. Morphology And Functions Of Astrocytes Cultured On Water-Repelle...

Chu H, Sun J, Xu H, Niu Z, Xu M. Neurol Res. 2012 Nov;34(9):871-88. Doi: 10.1179/1743132812Y.0000000085. Epub 2012 Aug 13. Effect Of Periaqueductal Gray Melanocortin 4 Receptor In Pain Facilitation And Glial Activation In Rat Model Of Chronic Cons...

Zhang Y, Sun Lg, Ye Lp, Wang B, Li Y. Toxicol Mech Methods. 2008 Jan;18(9):751-7. Doi: 10.1080/15376510802390908. Lead-Induced Stress Response In Endoplasmic Reticulum Of Astrocytes In Cns.

Huo Sj, Li Y, Raisman G, Yin Zq. Brain Res. 2011 Mar 25;1382:238-44. Doi: 10.1016/J.Brainres.2010.12.079. Epub 2011 Jan 6. Transplanted Olfactory Ensheathing Cells Reduce The Gliotic Injury Response Of M??ller Cells In A Rat Model Of Retinitis Pig...

Zhao Yy, Yuan Y, Chen Y, Jiang L, Liao Rj, Wang L, Zhang Xn, Ohtsu H, Hu Ww, Chen Z. Cns Neurosci Ther. 2015 May;21(5):454-62. Doi: 10.1111/Cns.12379. Epub 2015 Jan 24. Histamine Promotes Locomotion Recovery After Spinal Cord Hemisection Via Inhib...

Yuan H, Chen R, Wu L, Chen Q, Hu A, Zhang T, Wang Z, Zhu X. Mol Neurobiol. 2015 Apr;51(2):512-22. Doi: 10.1007/S12035-014-8717-6. Epub 2014 Apr 29. The Regulatory Mechanism Of Neurogenesis By Igf-1 In Adult Mice.

Wang R, Zhang X, Zhang J, Fan Y, Shen Y, Hu W, Chen Z. Plos One. 2012;7(5):E37574. Doi: 10.1371/Journal.Pone.0037574. Epub 2012 May 22. Oxygen-Glucose Deprivation Induced Glial Scar-Like Change In Astrocytes.

Zhang J, Gao Y, Shen Y, Li K. Indian J Med Res. 2009 Feb;129(2):164-9. Asymmetrical Release Of Interleukin-6 By Cultured Cerebral Cortical Astrocytes Treated With Lipopolysaccharide.

Shi Xh, Han J, Zhang J, Shi Q, Chen Jm, Xia Sl, Xie Zq, Shen Xj, Shan B, Lei Yj, Shi S, Zhou W, Zhang By, Gao C, Liu Yh, Song J, Guo Yj, Wang Dx, Xu Bl, Dong Xp. Infect Genet Evol. 2010 Mar;10(2):292-7. Doi: 10.1016/J.Meegid.2010.01.007. Epub 2010...

Zhang G, Zhao Z, Gao L, Deng J, Wang B, Xu D, Liu B, Qu Y, Yu J, Li J, Gao G. Pharmacol Biochem Behav. 2011 Jul;99(1):42-51. Doi: 10.1016/J.Pbb.2011.03.019. Epub 2011 Apr 1. Gypenoside Attenuates White Matter Lesions Induced By Chronic Cerebral Hy...

Ma H, Yu B, Kong L, Zhang Y, Shi Y. Mol Med Rep. 2011 Sep-Oct;4(5):849-56. Doi: 10.3892/Mmr.2011.510. Epub 2011 Jun 16. Transplantation Of Neural Stem Cells Enhances Expression Of Synaptic Protein And Promotes Functional Recovery In A Rat Model Of...

Zhang Gl, Deng Jp, Wang Bh, Zhao Zw, Li J, Gao L, Liu Bl, Xong Jr, Guo Xd, Yan Zq, Gao Gd. Behav Pharmacol. 2011 Oct;22(7):633-44. Doi: 10.1097/Fbp.0B013E32834Afef9. Gypenosides Improve Cognitive Impairment Induced By Chronic Cerebral Hypoperfusio...

Huile G, Shuaiqi P, Zhi Y, Shijie C, Chen C, Xinguo J, Shun S, Zhiqing P, Yu H. Biomaterials. 2011 Nov;32(33):8669-75. Doi: 10.1016/J.Biomaterials.2011.07.069. Epub 2011 Aug 16. A Cascade Targeting Strategy For Brain Neuroglial Cells Employing Nan...

Wu L, Miao S, Zou Lb, Wu P, Hao H, Tang K, Zeng P, Xiong J, Li Hh, Wu Q, Cai L, Ye Dy. J Mol Neurosci. 2012 Sep;48(1):185-200. Doi: 10.1007/S12031-012-9807-4. Epub 2012 Jun 3. Lipoxin A4 Inhibits 5-Lipoxygenase Translocation And Leukotrienes Biosy...

Chen Y, Wu X, Yu S, Lin X, Wu J, Li L, Zhao J, Zhao Y. Plos One. 2012;7(6):E40165. Doi: 10.1371/Journal.Pone.0040165. Epub 2012 Jun 29. Neuroprotection Of Tanshinone Iia Against Cerebral Ischemia/Reperfusion Injury Through Inhibition Of Macrophage...

Liu Lx, Zhou Xy, Li Cs, Liu Lq, Huang Sy, Zhou Sn. Neurol Sci. 2013 Sep;34(9):1671-8. Doi: 10.1007/S10072-013-1319-7. Epub 2013 Feb 13. Selenoprotein S Expression In The Rat Brain Following Focal Cerebral Ischemia.

Chen Z, Pan X, Georgakilas Ag, Chen P, Hu H, Yang Y, Tian S, Xia L, Zhang J, Cai X, Ge J, Yu K, Zhuang J. Cancer Lett. 2013 Aug 19;336(2):281-9. Doi: 10.1016/J.Canlet.2013.03.015. Epub 2013 Mar 21. Tetramethylpyrazine (Tmp) Protects Cerebral Neuro...

Chen X, Chen L, Chen J, Hu W, Gao H, Xie B, Wang X, Yin Z, Li S, Wang X. Brain Res. 2013 Nov 13;1538:151-8. Doi: 10.1016/J.Brainres.2013.02.025. Epub 2013 Mar 5. Adam17 Promotes U87 Glioblastoma Stem Cell Migration And Invasion.

Yu Q, Guo Z, Liu X, Ouyang Q, He C, Burnstock G, Yuan H, Xiang Z. Purinergic Signal. 2013 Dec;9(4):663-75. Doi: 10.1007/S11302-013-9379-Y. Epub 2013 Jul 23. Block Of P2X7 Receptors Could Partly Reverse The Delayed Neuronal Death In Area Ca1 Of The...

Sun Yx, Cong Yl, Liu Y, Jin B, Si L, Wang Ab, Cai H, Che Gy, Tang B, Wang Cf, Li Zy, Zhang Xm. Eur J Pharmacol. 2014 Oct 5;740:552-9. Doi: 10.1016/J.Ejphar.2014.06.032. Epub 2014 Jun 27. Schisandrin A And B Affect Subventricular Zone Neurogenesis ...

Xu X, Yang X, Xiong Y, Gu J, He C, Hu Y, Xiao F, Chen G, Wang X. J Neurochem. 2015 Apr;133(1):134-43. Doi: 10.1111/Jnc.13052. Epub 2015 Feb 26. Increased Expression Of Receptor For Activated C Kinase 1 In Temporal Lobe Epilepsy.

Lan Lf, Zheng L, Yang X, Ji Xt, Fan Yh, Zeng Js. Cns Neurosci Ther. 2015 May;21(5):410-6. Doi: 10.1111/Cns.12374. Epub 2015 Jan 22. Peroxisome Proliferator-Activated Receptor-?? Agonist Pioglitazone Ameliorates White Matter Lesion And Cognitive Im...

Zhang L, Li H, Zeng S, Chen L, Fang Z, Huang Q. Neurosci Lett. 2015 Mar 30;591:30-4. Doi: 10.1016/J.Neulet.2015.02.023. Epub 2015 Feb 11. Long-Term Tracing Of The Brdu Label-Retaining Cells In Adult Rat Brain.

Zhu X, Dong J, Shen K, Bai Y, Zhang Y, Lv X, Chao J, Yao H. Brain Res Bull. 2015 May;114:70-8. Doi: 10.1016/J.Brainresbull.2015.04.002. Epub 2015 Apr 17. Nmda Receptor Nr2B Subunits Contribute To Ptz-Kindling-Induced Hippocampal Astrocytosis And O...

Shi J, Ma Y, Zheng M, Ruan Z, Liu J, Tian S, Zhang D, He X, Li G. Toxicol Ind Health. 2012 Feb;28(1):10-20. Doi: 10.1177/0748233711401264. Epub 2011 Mar 28. Effect Of Sub-Acute Exposure To Acrylamide On Gabaergic Neurons And Astrocytes In Weaning ...

Li F, Miao Zn, Xu Yy, Zheng Sy, Qin Md, Gu Yz, Zhang Xg. Mol Med Rep. 2012 Sep;6(3):625-30. Doi: 10.3892/Mmr.2012.968. Epub 2012 Jun 26. Transplantation Of Human Amniotic Mesenchymal Stem Cells In The Treatment Of Focal Cerebral Ischemia.

Liu Q, Cheng G, Wang Z, Zhan S, Xiong B, Zhao X. In Vitro Cell Dev Biol Anim. 2015 Mar;51(3):319-27. Doi: 10.1007/S11626-015-9875-1. Epub 2015 Mar 14. Bone Marrow-Derived Mesenchymal Stem Cells Differentiate Into Nerve-Like Cells In Vitro After Tr...

Lu Y, Yuan X, Ou Y, Cai Y, Wang S, Sun Q, Zhang W. Neural Regen Res. 2012 Jun 5;7(16):1205-12. Doi: 10.3969/J.Issn.1673-5374.2012.16.001. Autophagy And Apoptosis During Adult Adipose-Derived Stromal Cells Differentiation Into Neuron-Like Cells In ...

Wang X, Tu Q, Zhao B, An Y, Wang Jc, Liu W, Yuan Ms, Ahmed Sm, Xu J, Liu R, Zhang Y, Wang J. Biomaterials. 2013 Jan;34(4):1155-69. Doi: 10.1016/J.Biomaterials.2012.10.063. Epub 2012 Nov 17. Effects Of Poly(L-Lysine)-Modified Fe3O4 Nanoparticles On...

Ping Yf, Yao Xh, Jiang Jy, Zhao Lt, Yu Sc, Jiang T, Lin Mc, Chen Jh, Wang B, Zhang R, Cui Yh, Qian C, Wang Jm, Bian Xw. J Pathol. 2011 Jul;224(3):344-54. Doi: 10.1002/Path.2908. Epub 2011 May 27. The Chemokine Cxcl12 And Its Receptor Cxcr4 Promote...

Wu Y, Ge C, Zeng W, Zhang C. Stem Cells Dev. 2010 Feb;19(2):195-202. Doi: 10.1089/Scd.2008.0383. Induced Multilineage Differentiation Of Chicken Embryonic Germ Cells Via Embryoid Body Formation.

Liu S, Zhang Y, Zhao Y, Cui H, Cao C, Guo J. Cell Biochem Biophys. 2015 Jan;71(1):401-6. Doi: 10.1007/S12013-014-0212-1. Effects Of Hypothermia On S100B And Glial Fibrillary Acidic Protein In Asphyxia Rats After Cardiopulmonary Resuscitation.

Ge Xt, Lei P, Wang Hc, Zhang Al, Han Zl, Chen X, Li Sh, Jiang Rc, Kang Cs, Zhang Jn. Sci Rep. 2014 Oct 24;4:6718. Doi: 10.1038/Srep06718. Mir-21 Improves The Neurological Outcome After Traumatic Brain Injury In Rats.

Meng Xt, Chen D, Dong Zy, Liu Jm. Cell Biol Int. 2007 Jul;31(7):691-8. Epub 2007 Jan 19. Enhanced Neural Differentiation Of Neural Stem Cells And Neurite Growth By Amniotic Epithelial Cell Co-Culture.

Longxi P, Buwu F, Yuan W, Sinan G. Plos One. 2011;6(10):E26500. Doi: 10.1371/Journal.Pone.0026500. Epub 2011 Oct 28. Expression Of P53 In The Effects Of Artesunate On Induction Of Apoptosis And Inhibition Of Proliferation In Rat Primary Hepatic St...

Niu Z, Ma J, Chu H, Zhao Y, Feng W, Cheng Y. Neurosci Lett. 2012 Nov 7;529(2):112-7. Doi: 10.1016/J.Neulet.2012.09.034. Epub 2012 Sep 27. Melanocortin 4 Receptor Antagonists Attenuates Morphine Antinociceptive Tolerance, Astroglial Activation And ...

Have you used Anti-GFAP Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

1 Reviews For Anti-GFAP Antibody

0

"This Antibody Works Perfectly!"

Excellent

Source: Biocompare.com

"This Antibody Works Perfectly!"

--Maria Teresa Dell'Anno, NEUROSCIENCE, YALE UNIVERSITY, POSTDOC

Applications Immunohistochemistry
Sample Spinal cord
Detection Confocal microscope

"I used it for IHC on frozen sections at a dilution of 1:500. It did not need several trials to optimize the protocol. No bad things overall. I will purchase it again."

Verified customer

Submitted

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

16 Customer Q&As for Anti-GFAP Antibody

Question

Is a blocking peptide available for product anti-GFAP antibody (PA1239)?

Verified Customer

Verified customer

Asked: 2020-05-06

Answer

We do provide the blocking peptide for product anti-GFAP antibody (PA1239). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-05-06

Question

Will PA1239 anti-GFAP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

You can see on the product datasheet, PA1239 anti-GFAP antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-22

Question

We have been able to see staining in rat fetal brain cortex. Any tips? Is anti-GFAP antibody supposed to stain fetal brain cortex positively?

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

From what I have seen in literature fetal brain cortex does express GFAP. From what I have seen in Uniprot.org, GFAP is expressed in dorsal motor nucleus of vagus nerve, brain thalamus, brain, kidney, fetal brain cortex, fetal brain, blood, among other tissues. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2020-02-18

Question

I have attached the WB image, lot number and protocol we used for fetal brain using anti-GFAP antibody PA1239. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-23

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-GFAP antibody PA1239. Let me know if you need anything else.

L. Rodriguez

Verified customer

Asked: 2019-12-16

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-16

Question

Can you help my question with product PA1239, anti-GFAP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-29

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1239 anti-GFAP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-29

Question

I was wanting to use your anti-GFAP antibody for IHC for rat fetal brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat fetal brain identification?

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

You can see on the product datasheet, PA1239 anti-GFAP antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fetal brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-22

Question

Is this PA1239 anti-GFAP antibody reactive to the isotypes of GFAP?

Z. Carter

Verified customer

Asked: 2019-07-15

Answer

The immunogen of PA1239 anti-GFAP antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human GFAP(417-432aa DGEVIKESKQEHKDVM), identical to the related rat sequence, and different from the related mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-07-15

Question

I see that the anti-GFAP antibody PA1239 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-12

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-12

Question

Is there a BSA free version of anti-GFAP antibody PA1239 available?

Verified Customer

Verified customer

Asked: 2019-05-15

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GFAP antibody PA1239 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-15

Question

I was wanting to use using your anti-GFAP antibody for regulation of chaperone-mediated autophagy studies. Has this antibody been tested with western blotting on u87 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-03-20

Answer

We appreciate your inquiry. This PA1239 anti-GFAP antibody is validated on rat brain tissue, tissue lysate, mouse brain, u87 whole cell lysate. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-03-20

Question

Will anti-GFAP antibody PA1239 work for IHC with fetal brain?

Verified Customer

Verified customer

Asked: 2018-12-20

Answer

According to the expression profile of fetal brain, GFAP is highly expressed in fetal brain. So, it is likely that anti-GFAP antibody PA1239 will work for IHC with fetal brain.

Boster Scientific Support

Answered: 2018-12-20

Question

We are currently using anti-GFAP antibody PA1239 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2018-07-11

Answer

The anti-GFAP antibody (PA1239) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-07-11

Question

I am looking for to test anti-GFAP antibody PA1239 on rat fetal brain for research purposes, then I may be interested in using anti-GFAP antibody PA1239 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-09-19

Answer

The products we sell, including anti-GFAP antibody PA1239, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-09-19

Question

We were content with the WB result of your anti-GFAP antibody. However we have seen positive staining in brain thalamus cytoplasm. using this antibody. Is that expected? Could you tell me where is GFAP supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-07-19

Answer

From literature, brain thalamus does express GFAP. Generally GFAP expresses in cytoplasm. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2017-07-19

Question

We purchased anti-GFAP antibody for IF on fetal brain in the past. I am using rat, and We are going to use the antibody for IHC next. I would like examining fetal brain as well as dorsal motor nucleus of vagus nerve in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

H. Dhar

Verified customer

Asked: 2016-04-15

Answer

I viewed the website and datasheets of our anti-GFAP antibody and it seems that PA1239 has been tested on rat in both IF and IHC. Thus PA1239 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2016-04-15

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
PA1239
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.