|Validated Species:||Human, Rabbit, Rat|
Data & Images
|Product Name||Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1)|
|Description||Mouse IgG monoclonal antibody for S-100 (beta-subunit), S100 calcium binding protein B (S100B) detection. Tested with IHC-P in Human;rabbit;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1) SH-B1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1088)|
|Replacement Item||This antibody may replace the following items: sc-136061|sc-28533|sc-393919|sc-398707|sc-52204|sc-52205|sc-52206|sc-58839|sc-71990|sc-71991|sc-7851|sc-7852|sc-81709 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rabbit, Rat|
|Predicted Species||Horse, Monkey, Rabbit|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Bovine brain S-100b.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Protein S100-B|
|Molecular Weight||10744 MW|
|Protein Function||Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. .|
|Tissue Specificity||Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. .|
|Sequence Similarities||Belongs to the S-100 family.|
|Subcellular Localization||Cytoplasm . Nucleus .|
|Alternative Names||Protein S100-B;S-100 protein beta chain;S-100 protein subunit beta;S100 calcium-binding protein B;S100b;|
|Research Areas|||cell biology|cell cycle|cell differentiation| epigenetics and nuclear signaling| stem cells|neural stem cells|glial restricted lineage|astrocyte||
Background for Protein S100-B
Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1) Images
Click the images to enlarge.
IHC(P): Rat Brain Tissue Lysate