Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1)
|Reactivity||Human, Rabbit, Rat|
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for S-100 (beta-subunit), S100 calcium binding protein B (S100B) detection. Tested with IHC-P in Human;rabbit;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1088)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Bovine brain S-100b.|
|Reactivity||Human, Rabbit, Rat|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, rabbit, rat, By Heat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
Images And Assay Conditions
Anti-S-100(beta-subunit) antibody (monoclonal), MA1088, IHC(P)
IHC(P): Rat Brain Tissue Lysate
Protein Target Info (Source: Uniprot.org)
|Protein Name||Protein S100-B|
|Tissue Specificity||Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. .|
|Alternative Names||Protein S100-B;S-100 protein beta chain;S-100 protein subunit beta;S100 calcium-binding protein B;S100b;|
|Subcellular Localization||Cytoplasm . Nucleus .|
|Molecular Weight||10744 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. .|
|Research Areas||Human, Rabbit, Rat
*You can search these to find other products in these research areas.
|Background||S100 protein is a 21,000-Da component first isolated from brain by Moore(1965). Immunocytochemical studies demonstrated that S100 protein is produced by a wide variety of normal and neoplastic cells of mesodermal, neuroectodermal, and epithelial origin(Herrera et al., 1988). The S100 molecule is a dimer; thus there are 3 forms of S100: alpha-alpha, known as S-100a(0); alpha-beta, known as S-100a; and beta-beta, known as S-100b. S100 genes are clustered on human chromosome 1q21. S-100 protein expression by primary and metastatic adenocarcinomas.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact firstname.lastname@example.org
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to email@example.com.
Q: Can you clarify the statement on this page "No cross reactivity with other proteins"?A: It means the antibody only recognizes S-100 beta and does not cross-react with any other cytokines.
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to s100 antibody, s100b antibody, nef antibody