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Pack Size:100μg/vial
Validated Species:Human, Rabbit, Rat
Clone Number:SH-B1
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Product Name Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1)
SKU/Catalog Number MA1088
Description Mouse IgG monoclonal antibody for S-100 (beta-subunit), S100 calcium binding protein B (S100B) detection. Tested with IHC-P in Human;rabbit;rat. No cross reactivity with other proteins.
Cite This Product Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1) SH-B1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1088)
Replacement Item This antibody may replace the following items: sc-136061|sc-28533|sc-393919|sc-398707|sc-52204|sc-52205|sc-52206|sc-58839|sc-71990|sc-71991|sc-7851|sc-7852|sc-81709 from Santa Cruz Biotechnology.
Host Mouse
Validated Species Human, Rabbit, Rat
Predicted Species Horse, Monkey, Rabbit

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen Bovine brain S-100b.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Monoclonal
Clone Number SH-B1
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name S100b
Protein Name Protein S100-B
Molecular Weight 10744 MW
Protein Function Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. .
Tissue Specificity Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. .
Sequence Similarities Belongs to the S-100 family.
Subcellular Localization Cytoplasm . Nucleus .
Uniprot ID P04631
Alternative Names Protein S100-B;S-100 protein beta chain;S-100 protein subunit beta;S100 calcium-binding protein B;S100b;
Research Areas |cell biology|cell cycle|cell differentiation| epigenetics and nuclear signaling| stem cells|neural stem cells|glial restricted lineage|astrocyte|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Protein S100-B

S100 protein is a 21,000-Da component first isolated from brain by Moore(1965). Immunocytochemical studies demonstrated that S100 protein is produced by a wide variety of normal and neoplastic cells of mesodermal, neuroectodermal, and epithelial origin(Herrera et al., 1988). The S100 molecule is a dimer; thus there are 3 forms of S100: alpha-alpha, known as S-100a(0); alpha-beta, known as S-100a; and beta-beta, known as S-100b. S100 genes are clustered on human chromosome 1q21. S-100 protein expression by primary and metastatic adenocarcinomas.

Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1) Images

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Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1)
Anti-S-100(beta-subunit) antibody (monoclonal), MA1088, IHC(P)
IHC(P): Rat Brain Tissue Lysate
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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: Can you clarify the statement on this page "No cross reactivity with other proteins"?
A: It means the antibody only recognizes S-100 beta and does not cross-react with any other cytokines.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to s100 antibody, s100b antibody, nef antibody