Anti-S-100(Beta-subunit) S100b Antibody (Monoclonal, SH-B1)

S100b antibody

Boster Bio Anti-S-100 (Beta-subunit) S100b Antibody (Monoclonal, SH-B1) catalog # MA1088. Tested in IHC applications. This antibody reacts with Human, Rabbit, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: MA1088
Size: 100 μg/vial
Reactive Species: Human, Rabbit, Rat
Host: Mouse
Application: IHC

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Product Name

Anti-S-100(Beta-subunit) S100b Antibody (Monoclonal, SH-B1)

View all S100b Antibodies

SKU/Catalog Number

MA1088

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-S-100 (Beta-subunit) S100b Antibody (Monoclonal, SH-B1) catalog # MA1088. Tested in IHC applications. This antibody reacts with Human, Rabbit, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-S-100(Beta-subunit) S100b Antibody (Monoclonal, SH-B1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1088)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

SH-B1

Isotype

Mouse IgG1

Immunogen

Bovine brain S-100b.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1088 is reactive to S100b in Human, Rabbit, Rat

Applications

MA1088 is guaranteed for IHC Boster Guarantee

Background of S100b

S100 protein is a 21,000-Da component first isolated from brain by Moore (1965). Immunocytochemical studies demonstrated that S100 protein is produced by a wide variety of normal and neoplastic cells of mesodermal, neuroectodermal, and epithelial origin (Herrera et al., 1988). The S100 molecule is a dimer; thus there are 3 forms of S100: alpha-alpha, known as S-100a (0); alpha-beta, known as S-100a; and beta-beta, known as S-100b. S100 genes are clustered on human chromosome 1q21. S-100 protein expression by primary and metastatic adenocarcinomas.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, rabbit, rat, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For S100b (Source: Uniprot.org, NCBI)

Uniprot ID

P04631

Gene ID

25742

Gene Name

S100b

Full Name

Protein S100-B

Weight

10.744kDa

Superfamily

S-100 family

Alternative Names

beta (neural); NEF; S100 beta; S100 calcium binding protein B; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain, 10protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100; S100B; S100beta S100b|S100P|S100 calcium binding protein B|protein S100-B|S-100 protein beta chain|S-100 protein subunit beta|S100 calcium-binding protein, beta (neural)|S100 protein, beta polypeptide, neural

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on S100b, check out the S100b Infographic

S100b infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for S100b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

MA1088 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Olfactory ensheathing cells promote nerve regeneration and functional recovery after facial nerve defects Jian Gu,1,et al. Neural Regen Res. 2019 Jan; 14(1): 124–131. doi: 10.4103/1673-5374.243717

Baicalin promotes the viability of Schwann cells in vitro by regulating neurotrophic factors

Expression of S100B during the innate immune of corneal epithelium against fungi invasion

A metanephric adenoma of the kidney associated with polycythemia: A case report

Dendritic cells and macrophages neurally hard-wired in the lymph node

Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury

Graft of a Tissue-Engineered Neural Scaffold Serves as a Promising Strategy to Restore Myelination after Rat Spinal Cord Transection

Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-?B signaling in an IL-6-dependent manner

Pro?neurogenic effects of andrographolide on RSC96 Schwann cells in?vitro

Different methods for inducing adipose-derived stem cells to differentiate into Schwann-like cells

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16 Customer Q&As for Anti-S-100(Beta-subunit) S100b Antibody (Monoclonal, SH-B1)

Question

Is a blocking peptide available for product anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) (MA1088)?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

We do provide the blocking peptide for product anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) (MA1088). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-05

Question

We have seen staining in rat skin. Any tips? Is anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) supposed to stain skin positively?

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

From what I have seen in literature skin does express S100B. From what I have seen in Uniprot.org, S100B is expressed in c1 segment of cervical spinal cord, skin, among other tissues. Regarding which tissues have S100B expression, here are a few articles citing expression in various tissues:
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-11-15

Question

Can you clarify the statement on this page "No cross reactivity with other proteins"?

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

It means the antibody only recognizes S-100 beta and does not cross-react with any other cytokines.

Boster Scientific Support

Answered: 2019-10-28

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for c1 segment of cervical spinal cord using anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088. Let me know if you need anything else.

N. Mitchell

Verified customer

Asked: 2019-10-02

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-02

Question

We are currently using anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 for rabbit tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, rabbit, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-02

Answer

The anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) (MA1088) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-02

Question

Does anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 work for IHC with c1 segment of cervical spinal cord?

Verified Customer

Verified customer

Asked: 2019-03-21

Answer

According to the expression profile of c1 segment of cervical spinal cord, S100B is highly expressed in c1 segment of cervical spinal cord. So, it is likely that anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 will work for IHC with c1 segment of cervical spinal cord.

Boster Scientific Support

Answered: 2019-03-21

Question

We are interested in using your anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) for response to methylmercury studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.

S. Jackson

Verified customer

Asked: 2019-03-18

Answer

Thanks for your inquiry. This MA1088 anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) is tested on rat brain tissue, tissue lysate. It is guaranteed to work for IHC in human, rabbit, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-03-18

Question

Here is the WB image, lot number and protocol we used for c1 segment of cervical spinal cord using anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088. Please let me know if you require anything else.

B. Johnson

Verified customer

Asked: 2019-02-14

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-02-14

Question

I was wanting to use your anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) for IHC for rat c1 segment of cervical spinal cord on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat c1 segment of cervical spinal cord identification?

Verified Customer

Verified customer

Asked: 2018-11-22

Answer

It shows on the product datasheet, MA1088 anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) has been validated for IHC on human, rabbit, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat c1 segment of cervical spinal cord in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-11-22

Question

Does MA1088 anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-03-29

Answer

As indicated on the product datasheet, MA1088 anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-03-29

Question

Is this MA1088 anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) reactive to the isotypes of S100B?

Verified Customer

Verified customer

Asked: 2017-10-06

Answer

The immunogen of MA1088 anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) is Bovine brain S-100b. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-10-06

Question

I have a question about product MA1088, anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-06-01

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1088 anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-06-01

Question

I see that the anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 works with IHC, what is the protocol used to produce the result images on the product page?

S. Jones

Verified customer

Asked: 2016-08-02

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-08-02

Question

We need to test anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 on rat c1 segment of cervical spinal cord for research purposes, then I may be interested in using anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

M. Anderson

Verified customer

Asked: 2015-08-19

Answer

The products we sell, including anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-08-19

Question

My boss were content with the WB result of your anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1). However we have seen positive staining in c1 segment of cervical spinal cord cytoplasm. using this antibody. Is that expected? Could you tell me where is S100B supposed to be expressed?

E. Gonzalez

Verified customer

Asked: 2013-12-16

Answer

From what I have seen in literature, c1 segment of cervical spinal cord does express S100B. Generally S100B expresses in cytoplasm. Regarding which tissues have S100B expression, here are a few articles citing expression in various tissues:
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2013-12-16

Question

Is there a BSA free version of anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 available?

T. Johnson

Verified customer

Asked: 2013-04-25

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-04-25

Size

Conjugation

Total: $315

SKU:MA1088

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MA1088
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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