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Product Info Summary
| SKU: | MA1088 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rabbit, Rat |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-S-100(Beta-subunit) S100b Antibody (Monoclonal, SH-B1)
SKU/Catalog Number
MA1088
BM0120 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-S-100 (Beta-subunit) S100b Antibody (Monoclonal, SH-B1) catalog # MA1088. Tested in IHC applications. This antibody reacts with Human, Rabbit, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-S-100(Beta-subunit) S100b Antibody (Monoclonal, SH-B1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1088)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
SH-B1
Isotype
Mouse IgG1
Immunogen
Bovine brain S-100b.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1088 is reactive to S100b in Human, Rabbit, Rat
Calculated molecular weight
10.7 kDa
Background of S100b
S100 protein is a 21,000-Da component first isolated from brain by Moore (1965). Immunocytochemical studies demonstrated that S100 protein is produced by a wide variety of normal and neoplastic cells of mesodermal, neuroectodermal, and epithelial origin (Herrera et al., 1988). The S100 molecule is a dimer; thus there are 3 forms of S100: alpha-alpha, known as S-100a (0); alpha-beta, known as S-100a; and beta-beta, known as S-100b. S100 genes are clustered on human chromosome 1q21. S-100 protein expression by primary and metastatic adenocarcinomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1088 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, rabbit, rat
Positive Control
IHC: Rat Brain Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-S-100(beta-subunit) antibody (monoclonal), MA1088, IHC(P)
IHC(P): Rat Brain Tissue Lysate
Specific Publications For Anti-S-100(Beta-subunit) S100b Antibody (Monoclonal, SH-B1) (MA1088)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-S-100(Beta-subunit) S100b Antibody (Monoclonal, SH-B1)
Question
Is a blocking peptide available for product anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) (MA1088)?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
We do provide the blocking peptide for product anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) (MA1088). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-05
Question
We have seen staining in rat skin. Any tips? Is anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) supposed to stain skin positively?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
From what I have seen in literature skin does express S100B. From what I have seen in Uniprot.org, S100B is expressed in c1 segment of cervical spinal cord, skin, among other tissues. Regarding which tissues have S100B expression, here are a few articles citing expression in various tissues:
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-15
Question
Can you clarify the statement on this page "No cross reactivity with other proteins"?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
It means the antibody only recognizes S-100 beta and does not cross-react with any other cytokines.
Boster Scientific Support
Answered: 2019-10-28
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for c1 segment of cervical spinal cord using anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088. Let me know if you need anything else.
N. Mitchell
Verified customer
Asked: 2019-10-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-02
Question
We are currently using anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 for rabbit tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, rabbit, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
The anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) (MA1088) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-02
Question
Does anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 work for IHC with c1 segment of cervical spinal cord?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
According to the expression profile of c1 segment of cervical spinal cord, S100B is highly expressed in c1 segment of cervical spinal cord. So, it is likely that anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 will work for IHC with c1 segment of cervical spinal cord.
Boster Scientific Support
Answered: 2019-03-21
Question
We are interested in using your anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) for response to methylmercury studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.
S. Jackson
Verified customer
Asked: 2019-03-18
Answer
Thanks for your inquiry. This MA1088 anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) is tested on rat brain tissue, tissue lysate. It is guaranteed to work for IHC in human, rabbit, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-03-18
Question
Here is the WB image, lot number and protocol we used for c1 segment of cervical spinal cord using anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088. Please let me know if you require anything else.
B. Johnson
Verified customer
Asked: 2019-02-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-14
Question
I was wanting to use your anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) for IHC for rat c1 segment of cervical spinal cord on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat c1 segment of cervical spinal cord identification?
Verified Customer
Verified customer
Asked: 2018-11-22
Answer
It shows on the product datasheet, MA1088 anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) has been validated for IHC on human, rabbit, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat c1 segment of cervical spinal cord in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-22
Question
Does MA1088 anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-03-29
Answer
As indicated on the product datasheet, MA1088 anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-29
Question
Is this MA1088 anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) reactive to the isotypes of S100B?
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
The immunogen of MA1088 anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) is Bovine brain S-100b. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-06
Question
I have a question about product MA1088, anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1088 anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-01
Question
I see that the anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 works with IHC, what is the protocol used to produce the result images on the product page?
S. Jones
Verified customer
Asked: 2016-08-02
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-08-02
Question
We need to test anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 on rat c1 segment of cervical spinal cord for research purposes, then I may be interested in using anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Anderson
Verified customer
Asked: 2015-08-19
Answer
The products we sell, including anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-08-19
Question
My boss were content with the WB result of your anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1). However we have seen positive staining in c1 segment of cervical spinal cord cytoplasm. using this antibody. Is that expected? Could you tell me where is S100B supposed to be expressed?
E. Gonzalez
Verified customer
Asked: 2013-12-16
Answer
From what I have seen in literature, c1 segment of cervical spinal cord does express S100B. Generally S100B expresses in cytoplasm. Regarding which tissues have S100B expression, here are a few articles citing expression in various tissues:
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-12-16
Question
Is there a BSA free version of anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 available?
T. Johnson
Verified customer
Asked: 2013-04-25
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-S-100(Beta-subunit) antibody (Monoclonal, SH-B1) MA1088 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-04-25
