|Reactivity||Human, Rabbit, Rat|
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for S-100 (beta-subunit), S100 calcium binding protein B (S100B) detection. Tested with IHC-P in Human;rabbit;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-S-100(Beta-subunit) Antibody (Monoclonal, SH-B1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1088)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Bovine brain S-100b.|
|Reactivity||Human, Rabbit, Rat|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, rabbit, rat, By Heat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
Images And Assay Conditions
Anti-S-100(beta-subunit) antibody (monoclonal), MA1088, IHC(P)
IHC(P): Rat Brain Tissue Lysate
Protein Target Info (Source: Uniprot.org)
|Protein Name||Protein S100-B|
|Tissue Specificity||Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. .|
|Alternative Names||Protein S100-B;S-100 protein beta chain;S-100 protein subunit beta;S100 calcium-binding protein B;S100b;|
|Subcellular Localization||Cytoplasm . Nucleus .|
|Molecular Weight||10744 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. .|
|Research Areas||Cell Biology, Cell Cycle, Cell Differentiation, Epigenetics And Nuclear Signaling, Neural Stem Cells, Stem Cells
*You can search these to find other products in these research areas.
|Background||S100 protein is a 21,000-Da component first isolated from brain by Moore(1965). Immunocytochemical studies demonstrated that S100 protein is produced by a wide variety of normal and neoplastic cells of mesodermal, neuroectodermal, and epithelial origin(Herrera et al., 1988). The S100 molecule is a dimer; thus there are 3 forms of S100: alpha-alpha, known as S-100a(0); alpha-beta, known as S-100a; and beta-beta, known as S-100b. S100 genes are clustered on human chromosome 1q21. S-100 protein expression by primary and metastatic adenocarcinomas.|
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