Product Info Summary
SKU: | A11256-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GHITM Antibody Picoband™
SKU/Catalog Number
A11256-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GHITM Antibody Picoband™ catalog # A11256-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GHITM Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11256-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GHITM recombinant protein (Position: M1-K345).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A11256-1 is reactive to GHITM in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
Background of GHITM
Growth hormone-inducible transmembrane protein (GHITM), also known as transmembrane BAX inhibitor motif containing protein 5 (TMBIM5), is a protein that in humans is encoded by the GHITM gene on chromosome 10. GHITM, also known asMICS1, TMBIM5 orDERP2, is a mitochondrial protein which localizes in the inner membrane.GHITM is involved in mitochondrial morphology in specific cristae structures and the apoptotic release of cytochrome c from the mitochondria. The gene of GHITM maps to chromosome 10q23.1, and encodes a 345-amino-acid protein with a calculated molecular mass of 37 kDa. The apparent molecular weight has been reported to be 42 kDa, the increased size in the protein may be due to post-translational modifications. GHITM can be cleaved into smaller forms of 23-27 kDa.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11256-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Positive Control
Validation Images & Assay Conditions
![a11256 1 ghitm primary antibodies wb testing 1 a11256 1 ghitm primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a11256-1-ghitm-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of GHITM using anti-GHITM antibody (A11256-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GHITM antigen affinity purified polyclonal antibody (Catalog # A11256-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GHITM at approximately 37 kDa. The expected band size for GHITM is at 37 kDa.
![a11256 1 ghitm primary antibodies ihc testing 2 a11256 1 ghitm primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a11256-1-ghitm-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of GHITM using anti-GHITM antibody (A11256-1).
GHITM was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GHITM Antibody (A11256-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a11256 1 ghitm primary antibodies ihc testing 3 a11256 1 ghitm primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/1/a11256-1-ghitm-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of GHITM using anti-GHITM antibody (A11256-1).
GHITM was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GHITM Antibody (A11256-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a11256 1 ghitm primary antibodies ihc testing 4 a11256 1 ghitm primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/1/a11256-1-ghitm-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of GHITM using anti-GHITM antibody (A11256-1).
GHITM was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GHITM Antibody (A11256-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a11256 1 ghitm primary antibodies fcm testing 5 a11256 1 ghitm primary antibodies fcm testing 5](https://www.bosterbio.com/media/catalog/product/a/1/a11256-1-ghitm-primary-antibodies-fcm-testing-5.png)
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Figure 5. Flow Cytometry analysis of A431 cells using anti-GHITM antibody (A11256-1).
Overlay histogram showing A431 cells stained with A11256-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GHITM Antibody (A11256-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![a11256 1 bhitm primary antibodies if testing 6 a11256 1 bhitm primary antibodies if testing 6](https://www.bosterbio.com/media/catalog/product/a/1/a11256-1-bhitm-primary-antibodies-if-testing-6.jpg)
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Figure 6. IF analysis of GHITM using anti-GHITM antibody (A11256-1).
GHITM was detected in an immunocytochemical section of HEP3B cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GHITM Antibody (A11256-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For GHITM (Source: Uniprot.org, NCBI)
Gene Name
GHITM
Full Name
Growth hormone-inducible transmembrane protein
Weight
Superfamily
BI1 family
Alternative Names
Dermal papilla-derived protein 2; DERP2MICS1; DKFZp566C0746; FLJ26584; growth hormone inducible transmembrane protein; growth hormone-inducible transmembrane protein; HSPC282; Mitochondrial morphology and cristae structure 1; PTD010; TMBIM5My021; transmembrane BAX inhibitor motif containing 5; Transmembrane BAX inhibitor motif-containing protein 5 GHITM DERP2, HSPC282, MICS1, My021, PTD010, TMBIM5 growth hormone inducible transmembrane protein growth hormone-inducible transmembrane protein|dermal papilla-derived protein 2|mitochondrial morphology and cristae structure 1|transmembrane BAX inhibitor motif containing 5|transmembrane BAX inhibitor motif-containing protein 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GHITM, check out the GHITM Infographic
![GHITM infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GHITM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GHITM Antibody Picoband™ (A11256-1)
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