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Product Info Summary
| SKU: | M04969 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GIPC (GIPC1) Mouse Monoclonal Antibody [Clone ID: OTI5C10]
SKU/Catalog Number
M04969
Size
100 µl
Description
Boster Bio GIPC1 mouse monoclonal antibody,clone OTI5C10. Catalog# M04969. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-GIPC (GIPC1) Mouse Monoclonal Antibody [Clone ID: OTI5C10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04969)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI5C10
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human GIPC1 (NP_974199) produced in E.coli.
Reactive Species
M04969 is reactive to GIPC1 in Human, Mouse, Rat
Calculated molecular weight
36.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04969 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000, IHC 1:500
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GIPC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GIPC1.
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Western blot analysis of extracts (35ug) from Hela cell line by using anti-GIPC1 monoclonal antibody (1:500).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GIPC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-GIPC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and GIPC1-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-GIPC1 monoclonal antibody M04969 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Specific Publications For Anti-GIPC (GIPC1) Mouse Monoclonal Antibody [Clone ID: OTI5C10] (M04969)
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