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Product Info Summary
| SKU: | A04441 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GIT2 Antibody
SKU/Catalog Number
A04441
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GIT2 Antibody catalog # A04441. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GIT2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04441)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human GIT2. at AA rangle: 330-410
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04441 is reactive to GIT2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
84543 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04441 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Positive Control
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HepG2 cells using GIT2 Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using GIT2 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For GIT2 (Source: Uniprot.org, NCBI)
Gene Name
GIT2
Full Name
ARF GTPase-activating protein GIT2
Weight
84543 MW
Alternative Names
ARF GAP GIT2; ARF GTPase-activating protein GIT2; CAT2; CAT-2; cool-associated, tyrosine phosphorylated protein 2; Cool-interacting tyrosine-phosphorylated protein 2; DKFZp686G01261; G protein-coupled receptor kinase interacting ArfGAP 2; G protein-coupled receptor kinase interactor 2; G protein-coupled receptor kinase-interactor 2; KIAA0148GRK-interacting protein 2; MGC760 GIT2 CAT-2, CAT2, PKL GIT ArfGAP 2 ARF GTPase-activating protein GIT2|ARF GAP GIT2|G protein-coupled receptor kinase interacting ArfGAP 2|G protein-coupled receptor kinase-interactor 2|GRK-interacting protein 2|Paxillin kinase linker|cool-associated, tyrosine phosphorylated protein 2|cool-interacting tyrosine-phosphorylated protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GIT2, check out the GIT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GIT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GIT2 Antibody (A04441)
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No publications found for A04441
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-GIT2 Antibody
Question
We are interested in to test anti-GIT2 antibody A04441 on human bone marrow for research purposes, then I may be interested in using anti-GIT2 antibody A04441 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
The products we sell, including anti-GIT2 antibody A04441, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bone marrow using anti-GIT2 antibody A04441. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-03
Question
I was wanting to use your anti-GIT2 antibody for WB for human bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bone marrow identification?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
It shows on the product datasheet, A04441 anti-GIT2 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-18
Question
Is this A04441 anti-GIT2 antibody reactive to the isotypes of GIT2?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
The immunogen of A04441 anti-GIT2 antibody is Synthesized peptide derived from the Internal region of human GIT2. at AA rangle: 330-410. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-06
Question
Does A04441 anti-GIT2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
As indicated on the product datasheet, A04441 anti-GIT2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-03
Question
Will anti-GIT2 antibody A04441 work on primate WB with bone marrow?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
Our lab technicians have not validated anti-GIT2 antibody A04441 on primate. You can run a BLAST between primate and the immunogen sequence of anti-GIT2 antibody A04441 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate bone marrow in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-24
Question
I see that the anti-GIT2 antibody A04441 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-02
Question
Is a blocking peptide available for product anti-GIT2 antibody (A04441)?
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
We do provide the blocking peptide for product anti-GIT2 antibody (A04441). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-30
Question
See attached the WB image, lot number and protocol we used for bone marrow using anti-GIT2 antibody A04441. Please let me know if you require anything else.
B. Gonzalez
Verified customer
Asked: 2018-01-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-29
Question
Would anti-GIT2 antibody A04441 work for WB with bone marrow?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
According to the expression profile of bone marrow, GIT2 is highly expressed in bone marrow. So, it is likely that anti-GIT2 antibody A04441 will work for WB with bone marrow.
Boster Scientific Support
Answered: 2017-11-13
Question
I have a question about product A04441, anti-GIT2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Banerjee
Verified customer
Asked: 2017-04-19
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04441 anti-GIT2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-04-19
Question
Is there a BSA free version of anti-GIT2 antibody A04441 available?
S. Moore
Verified customer
Asked: 2016-11-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GIT2 antibody A04441 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-11-28
Question
We are currently using anti-GIT2 antibody A04441 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
H. Dhar
Verified customer
Asked: 2016-04-15
Answer
The anti-GIT2 antibody (A04441) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-04-15
