Human Kallikrein 14 ELISA Kit PicoKine™

Human Kallikrein 14 ELISA Kit PicoKine™ (96 Tests). Quantitate Human KLK14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2 pg/ml.

Product Info Summary

SKU: EK0949
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human Kallikrein 14 ELISA Kit PicoKine™

See all Kallikrein 14 products

SKU/Catalog Number

EK0949

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Kallikrein 14 ELISA Kit PicoKine™ (96 Tests). Quantitate Human KLK14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Kallikrein 14 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0949)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q19-M248

Sensitivity

<2 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0949 is reactive to KLK14 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0949 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0949-CAP Anti-Human KLK14 Pre-coated 96-well strip microplate 1
EK0949-ST Human KLK14 Standard 2 vials, 10 ng/tube
EK0949-DA Human KLK14 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0150.0610.0960.1600.3180.5871.1201.967

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Kallikrein 14 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2821151229216500
Standard deviation2.110.1229.692.7213.3937.5
CV (%)7.5%4.8%5.8%9.4%6.2%7.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Kallikrein 14 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 128293331301.926.4%
Sample 221118420120520010.035%
Sample 351248943046447330.436.4%
*number of samples for each test n=16.

Gene/Protein Information For KLK14 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9P0G3

Gene ID

43847

Gene Name

KLK14

Full Name

Kallikrein-14

Weight

29.122kDa

Superfamily

peptidase S1 family

Alternative Names

EC 3.4.21; EC 3.4.21.-; hK14; Kallikrein 14; Kallikrein-like protein 6; kallikrein-related peptidase 14; KLK14; KLKL6; KLK-L6kallikrein-14 KLK14 KLK-L6 kallikrein related peptidase 14 kallikrein-14|kallikrein-like protein 6

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on KLK14, check out the KLK14 Infographic

KLK14 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for KLK14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK0949

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9 Customer Q&As for Human Kallikrein 14 ELISA Kit PicoKine™

Question

Q: Can Kallikrein 14 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

M. Gonzalez

Verified customer

Asked: 2020-12-13

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-12-13

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-08-21

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-08-21

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Kallikrein 14 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-06-27

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Kallikrein 14 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-06-27

Question

Re: EK0949 Human Kallikrein 14 PicoKine ELISA Kit Please send the current manual. A customer would like to know if samples containing phosphatase inhibitors are compatible with this assay.

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

Cell lysate samples containing phosphatase inhibitors will be compatible with EK0949.

Boster Scientific Support

Answered: 2020-02-21

Question

What samples that contain phosphatase inhibitors will be compatible with Human Kallikrein 14 ELISA Kit PicoKine?

Verified customer

Asked: 2019-11-14

Answer

Cell lysate samples containing phosphatase inhibitors will be compatible with Human Kallikrein 14 ELISA Kit PicoKine.

Boster Scientific Support

Answered: 2019-11-14

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-09-18

Question

Q: if the enzyme conjugated Kallikrein 14 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Kallikrein 14 antigen?

L. Green

Verified customer

Asked: 2019-04-19

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-04-19

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

B. Young

Verified customer

Asked: 2018-11-06

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-11-06

Question

Q: can I use citrate plasma as samples in Human Kallikrein 14 Picokine® ELISA Kit (Catalog # EK0949)?

K. Campbell

Verified customer

Asked: 2018-09-14

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Kallikrein 14 causing degradation of its protein structure. Kallikrein 14 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Kallikrein 14 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-09-14

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