Anti-GLCNE GNE Antibody

N-Acetylmannosamine Kinase/GNE antibody

Boster Bio Anti-GLCNE GNE Antibody catalog # A01647-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01647-1
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Customers Who Bought This Also Bought

Product Name

Anti-GLCNE GNE Antibody

View all N-Acetylmannosamine Kinase/GNE Antibodies

SKU/Catalog Number

A01647-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-GLCNE GNE Antibody catalog # A01647-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GLCNE GNE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01647-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

2C5

Isotype

IgG

Immunogen

Synthesized peptide derived from the C-terminal region of human GLCNE. at AA rangle: 570-650

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01647-1 is reactive to GNE in Human, Mouse, Rat

Applications

A01647-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

79.275kDa

Background of N-Acetylmannosamine Kinase/GNE

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Fulton, D. et al. (1999) Nature 399, 597-601.
Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For GNE (Source: Uniprot.org, NCBI)

Gene Name

GNE

Full Name

Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase

Weight

79.275kDa

Alternative Names

bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; DMRV; GLCNE; GLCNENM; glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase; GNE; IBM2; NAcetylmannosamine Kinase; N-Acetylmannosamine Kinase; N-acylmannosamine kinase; Uae1; UDP-GlcNAc-2-epimerase/ManAc kinase; UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase GNE DMRV, GLCNE, IBM2, NM, Uae1 glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase|N-acylmannosamine kinase|UDP-GlcNAc-2-epimerase/ManAc kinase|UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GNE, check out the GNE Infographic

GNE infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GNE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A01647-1

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-GLCNE GNE Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-GLCNE GNE Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Anti-GLCNE GNE Antibody

Question

Is a blocking peptide available for product anti-GLCNE antibody (A01647-1)?

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

We do provide the blocking peptide for product anti-GLCNE antibody (A01647-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-01

Question

See attached the WB image, lot number and protocol we used for small intestine using anti-GLCNE antibody A01647-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-10

Question

you antibody to test anti-GLCNE antibody A01647-1 on mouse small intestine for research purposes, then I may be interested in using anti-GLCNE antibody A01647-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

The products we sell, including anti-GLCNE antibody A01647-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-20

Question

Will A01647-1 anti-GLCNE antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Edwards

Verified customer

Asked: 2020-01-06

Answer

You can see on the product datasheet, A01647-1 anti-GLCNE antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-06

Question

Does anti-GLCNE antibody A01647-1 work on bovine WB with hippocampus tongue?

Verified Customer

Verified customer

Asked: 2018-01-17

Answer

Our lab technicians have not tested anti-GLCNE antibody A01647-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-GLCNE antibody A01647-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine hippocampus tongue in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-17

Question

We are currently using anti-GLCNE antibody A01647-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

D. Li

Verified customer

Asked: 2015-04-09

Answer

The anti-GLCNE antibody (A01647-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-04-09

Question

I see that the anti-GLCNE antibody A01647-1 works with WB, what is the protocol used to produce the result images on the product page?

T. Patel

Verified customer

Asked: 2015-01-19

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-01-19

Order DetailsPrice
A01647-1

100uL

$399

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
A01647-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.