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- Table of Contents
VCP antibody (PA2137) performs well in Flow Cytometry, IF, IHC, IHC-F, ICC, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of HELA cells using anti-VCP antibody (PA2137).
Overlay histogram showing HELA cells stained with PA2137 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VCP Antibody (PA2137, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HELA cells stained with PA2137 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VCP Antibody (PA2137, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-VCP Antibody Picoband®
VCP antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
Tested Applications
VCP antibody was positively detected by WB in:
human Hela whole cell, human SH-SY5Y whole cell, human MCF-7 whole cell, human A431 whole cell, human K562 whole cell, human HepG2 whole cell, monkey COS-7 whole cell, rat brain tissue, rat lung tissue, rat stomach tissue, rat C6 whole cell, mouse brain tissue, mouse lung tissue, mouse RAW2647 whole cell, human Hela whole cell, human SH-SY5Y whole cell, human MCF-7 whole cell, human A431 whole cell, human HepG2 whole cell, human K562 whole cell, human U87 whole cell, rat brain tissue, rat lung tissue, rat kidney tissue, rat C6 whole cell, mouse brain tissue, mouse lung tissue, mouse kidney tissue, mouse RAW2647 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
VCP antibody was positively detected by IHC in:
Human Mammary Cancer tissue, Rat Cerebellum tissue, Rat Epinephros tissue, Rat Intestine tissue, mouse intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
VCP antibody was positively detected by IHC-F in:
Rat Intestine tissue
VCP antibody was positively detected by ICC/IF in:
A431 cell
VCP antibody was positively detected by ICC in:
K562 cell
VCP antibody was positively detected by FC in:
HELA cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Rat
Immunocytochemistry
0.5-1μg/ml
Human
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Tested
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Fruit fly
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-VCP Antibody Picoband®
View all VCP Antibodies
View all VCP Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human VCP, identical to the related rat and mouse sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
89 kDa
Calculated Molecular Weight
89.3 kDa
Background of VCP
Valosin-containing protein also called CDC48 is an enzyme that in humans is encoded by the VCP gene. It is a member of the AAA+ (ATPase associated with various activities) protein family. The VCP gene maps to chromosome 9p13.3. It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. It is involved in the formation of the transitional endoplasmic reticulum. This gene plays a role in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. It also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites.
Cite This Product
Anti-VCP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2137)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Zebrafish
100%
High Homology
Drosophila
100%
High Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for VCP antibody
Immunohistochemistry (IHC) protocol for VCP antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for VCP antibody
Immunocytochemistry (ICC) protocol for VCP antibody
Flow Cytometry (FC) protocol for VCP antibody
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PA2137
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 18, 2026