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- Table of Contents
GLS2 antibody (A05334) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence Validation of GLS2 in Mouse Brain
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling GLS2 with A05334 at 20 μg g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling GLS2 with A05334 at 20 μg g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
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Anti-GLS2 Antibody
GLS2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
WB: 0.5-2 μg/mL; IHC-P: 0.5-2 μg/mL; IHC-P/IF: 10-20 μg/mL.
Antibody validated: Western Blot in human
mouse and rat samples; Immunohistochemistry in human
mouse, and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-GLS2 Antibody
View all GLS2 Antibodies
View all GLS2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
GLS2 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
GLS2 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2. The immunogen is located within amino acids 300 - 350 of GLS2.
Cross-Reactivity
Multiple isoforms of GLS2 are known to exist.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
66.3 kDa
Background of GLS2
Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53's role in antioxidant defense in addition to its role in energy metabolism.
Cite This Product
Anti-GLS2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05334)
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Product Promise
We promise Anti-GLS2 Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A05334
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026