Anti-GM130 GOLGA2 Antibody Picoband™ (monoclonal, 6D4)

Boster Bio Anti-GM130 GOLGA2 Antibody Picoband™ (monoclonal, 6D4) catalog # M05865-2. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M05865-2
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-GM130 GOLGA2 Antibody Picoband™ (monoclonal, 6D4)

See all GM130/GOLGA2 products

SKU/Catalog Number

M05865-2

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GM130 GOLGA2 Antibody Picoband™ (monoclonal, 6D4) catalog # M05865-2. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GM130 GOLGA2 Antibody Picoband™ (monoclonal, 6D4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05865-2)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

6D4

Isotype

IgG1

Immunogen

E. coli-derived human GM130 recombinant protein (Position: E796-E913).

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M05865-2 is reactive to GOLGA2 in Human, Mouse, Rat

Applications

M05865-2 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For GOLGA2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q08379

Gene ID

2801

Gene Name

GOLGA2

Full Name

Golgin subfamily A member 2

Weight

113.086kDa

Superfamily

GOLGA2 family

Alternative Names

130 kDa cis-Golgi matrix protein; GM130 autoantigen; GM130; GM130Golgi matrix protein GM130; GOLGA2; golgi autoantigen, golgin subfamily a, 2; golgin A2; Golgin subfamily A member 2; Golgin-95; MGC20672; SY11 Protein GOLGA2 GM130 golgin A2 golgin subfamily A member 2|130 kDa cis-Golgi matrix protein|GM130 autoantigen|Golgi matrix protein GM130|SY11 protein|golgi autoantigen, golgin subfamily a, 2|golgin-95

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GOLGA2, check out the GOLGA2 Infographic

GOLGA2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GOLGA2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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15 Customer Q&As for Anti-GM130 GOLGA2 Antibody Picoband™ (monoclonal, 6D4)

Question

My colleagues were well pleased with the WB result of your anti-GM130 antibody (monoclonal, 6D4). However we have seen positive staining in cervix carcinoma golgi apparatus using this antibody. Is that expected? Could you tell me where is GOLGA2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-03-20

Answer

From what I have seen in literature, cervix carcinoma does express GOLGA2. Generally GOLGA2 expresses in golgi apparatus, cis-golgi network membrane. Regarding which tissues have GOLGA2 expression, here are a few articles citing expression in various tissues:
Brain, and Colon adenocarcinoma, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 8315394, 24275569
Placenta, Pubmed ID: 11306556

Boster Scientific Support

Answered: 2020-03-20

Question

Please see the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-GM130 antibody (monoclonal, 6D4) M05865-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-10

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-GM130 antibody (monoclonal, 6D4) M05865-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-07

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-07

Question

I was wanting to use your anti-GM130 antibody (monoclonal, 6D4) for IF for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

You can see on the product datasheet, M05865-2 anti-GM130 antibody (monoclonal, 6D4) has been tested for Flow Cytometry, IF, IHC-P, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-22

Question

We ordered your anti-GM130 antibody (monoclonal, 6D4) for IF on brain colon adenocarcinoma in the past. I am using human, and We are going to use the antibody for WB next. I am interested in examining brain colon adenocarcinoma as well as cervix carcinoma erythroleukemia in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-07-16

Answer

I looked at the website and datasheets of our anti-GM130 antibody (monoclonal, 6D4) and it seems that M05865-2 has been validated on human in both IF and WB. Thus M05865-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-16

Question

Does anti-GM130 antibody (monoclonal, 6D4) M05865-2 work for IF with cervix carcinoma erythroleukemia?

E. Johnson

Verified customer

Asked: 2019-03-07

Answer

According to the expression profile of cervix carcinoma erythroleukemia, GOLGA2 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-GM130 antibody (monoclonal, 6D4) M05865-2 will work for IF with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2019-03-07

Question

Is a blocking peptide available for product anti-GM130 antibody (monoclonal, 6D4) (M05865-2)?

Verified Customer

Verified customer

Asked: 2018-10-16

Answer

We do provide the blocking peptide for product anti-GM130 antibody (monoclonal, 6D4) (M05865-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-10-16

Question

Would M05865-2 anti-GM130 antibody (monoclonal, 6D4) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

S. Carter

Verified customer

Asked: 2018-06-22

Answer

As indicated on the product datasheet, M05865-2 anti-GM130 antibody (monoclonal, 6D4) as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-06-22

Question

Is there a BSA free version of anti-GM130 antibody (monoclonal, 6D4) M05865-2 available?

Verified Customer

Verified customer

Asked: 2018-03-19

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GM130 antibody (monoclonal, 6D4) M05865-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-03-19

Question

Our lab want to know about to test anti-GM130 antibody (monoclonal, 6D4) M05865-2 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-GM130 antibody (monoclonal, 6D4) M05865-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-03-15

Answer

The products we sell, including anti-GM130 antibody (monoclonal, 6D4) M05865-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-03-15

Question

We have been able to see staining in human brain colon adenocarcinoma. Any tips? Is anti-GM130 antibody (monoclonal, 6D4) supposed to stain brain colon adenocarcinoma positively?

Verified Customer

Verified customer

Asked: 2017-12-25

Answer

From what I have seen in literature brain colon adenocarcinoma does express GOLGA2. From what I have seen in Uniprot.org, GOLGA2 is expressed in adenohypophysis, liver, brain colon adenocarcinoma, placenta, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have GOLGA2 expression, here are a few articles citing expression in various tissues:
Brain, and Colon adenocarcinoma, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 8315394, 24275569
Placenta, Pubmed ID: 11306556

Boster Scientific Support

Answered: 2017-12-25

Question

Is this M05865-2 anti-GM130 antibody (monoclonal, 6D4) reactive to the isotypes of GOLGA2?

S. Parker

Verified customer

Asked: 2017-06-30

Answer

The immunogen of M05865-2 anti-GM130 antibody (monoclonal, 6D4) is E. coli-derived human GM130 recombinant protein (Position: E796-E913). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-06-30

Question

We are currently using anti-GM130 antibody (monoclonal, 6D4) M05865-2 for rat tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

R. Johnson

Verified customer

Asked: 2016-11-07

Answer

The anti-GM130 antibody (monoclonal, 6D4) (M05865-2) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-11-07

Question

Can you help my question with product M05865-2, anti-GM130 antibody (monoclonal, 6D4). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

P. Kulkarni

Verified customer

Asked: 2015-02-05

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05865-2 anti-GM130 antibody (monoclonal, 6D4), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-02-05

Question

I see that the anti-GM130 antibody (monoclonal, 6D4) M05865-2 works with IF, what is the protocol used to produce the result images on the product page?

L. Lewis

Verified customer

Asked: 2015-01-19

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-01-19

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