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Product Info Summary
| SKU: | MA1095 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Chicken, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-Tropomyosin(36/39 kDa) Tpm1 Antibody (Monoclonal, TM31)
SKU/Catalog Number
MA1095
BM1440 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tropomyosin (36/39 kDa) Tpm1 Antibody (Monoclonal, TM31) catalog # MA1095. Tested in WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tropomyosin(36/39 kDa) Tpm1 Antibody (Monoclonal, TM31) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1095)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
TM31
Isotype
Mouse IgG1
Immunogen
Chicken gizzard tropomyosin.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1095 is reactive to Tpm1 in Chicken, Human, Mouse, Rat
Observed Molecular Weight
35-45 kDa
Calculated molecular weight
32.7 kDa
Background of Tpm1
The tropomyosins are a family of actin filament binding proteins. These proteins were first isolated from skeletal muscle, but later identified in many nonmuscle tissues. Tropomyosins are ubiquitous proteins of 35 to 45 kD associated with the actin filaments of myofibrils and stress fibers. Vertebrates have at least 4 different tropomyosin genes; in humans, they are named TPM1, TPM2, TPM3, and TPM4. Tropomyosins expressed as different isoforms in muscle and non-muscle cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1095 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.5-1μg/ml, Human, mouse, rat, chicken
Positive Control
WB: rat skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue, mouse heart tissue
Validation Images & Assay Conditions
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Western blot analysis of Tropomyosin(36/39kDa) using anti-Tropomyosin(36/39kDa) antibody (MA1095).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse skeletal muscle tissue lysates,
Lane 4: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Tropomyosin(36/39kDa) antigen affinity purified monoclonal antibody (Catalog # MA1095) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tropomyosin(36/39kDa) at approximately 35-45 kDa. The expected band size for Tropomyosin(36/39kDa) is at 33 kDa.
Specific Publications For Anti-Tropomyosin(36/39 kDa) Tpm1 Antibody (Monoclonal, TM31) (MA1095)
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Customer Q&As
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7 Customer Q&As for Anti-Tropomyosin(36/39 kDa) Tpm1 Antibody (Monoclonal, TM31)
Question
Is there a BSA free version of anti-Tropomyosin(36/39 kDa) antibody (Monoclonal, TM31) MA1095 available?
A. Anderson
Verified customer
Asked: 2020-04-21
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Tropomyosin(36/39 kDa) antibody (Monoclonal, TM31) MA1095 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-21
Question
I was wanting to use your anti-Tropomyosin(36/39 kDa) antibody (Monoclonal, TM31) for WB for chicken uterus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for chicken uterus identification?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
It shows on the product datasheet, MA1095 anti-Tropomyosin(36/39 kDa) antibody (Monoclonal, TM31) has been validated for WB on chicken, human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in chicken uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-09
Question
I see that the anti-Tropomyosin(36/39 kDa) antibody (Monoclonal, TM31) MA1095 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-12
Question
I have a question about product MA1095, anti-Tropomyosin(36/39 kDa) antibody (Monoclonal, TM31). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Jones
Verified customer
Asked: 2017-11-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1095 anti-Tropomyosin(36/39 kDa) antibody (Monoclonal, TM31), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-21
Question
Does anti-Tropomyosin(36/39 kDa) antibody (Monoclonal, TM31) MA1095 work on pig WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2017-11-10
Answer
Our lab technicians have not validated anti-Tropomyosin(36/39 kDa) antibody (Monoclonal, TM31) MA1095 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Tropomyosin(36/39 kDa) antibody (Monoclonal, TM31) MA1095 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-10
Question
I am interested in to test anti-Tropomyosin(36/39 kDa) antibody (Monoclonal, TM31) MA1095 on chicken uterus for research purposes, then I may be interested in using anti-Tropomyosin(36/39 kDa) antibody (Monoclonal, TM31) MA1095 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Williams
Verified customer
Asked: 2017-09-12
Answer
The products we sell, including anti-Tropomyosin(36/39 kDa) antibody (Monoclonal, TM31) MA1095, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-12
Question
Here is the WB image, lot number and protocol we used for uterus using anti-Tropomyosin(36/39 kDa) antibody (Monoclonal, TM31) MA1095. Please let me know if you require anything else.
L. Johnson
Verified customer
Asked: 2015-04-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-29
