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Product Info Summary
| SKU: | A14528 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GPR113 Antibody
SKU/Catalog Number
A14528
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GPR113 Antibody catalog # A14528. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR113 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14528)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR113. AA range:21-70
Reactive Species
A14528 is reactive to ADGRF3 in Human, Mouse
Calculated molecular weight
116.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14528 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot analysis of Hela cells using GPR113 Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from LOVO cells, using GPR113 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR113 Antibody (A14528)
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5 Customer Q&As for Anti-GPR113 Antibody
Question
I have a question about product A14528, anti-GPR113 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14528 anti-GPR113 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-31
Question
Would A14528 anti-GPR113 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
As indicated on the product datasheet, A14528 anti-GPR113 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-31
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellum using anti-GPR113 antibody A14528. Let me know if you need anything else.
M. Anderson
Verified customer
Asked: 2017-06-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-26
Question
We want to test anti-GPR113 antibody A14528 on human cerebellum for research purposes, then I may be interested in using anti-GPR113 antibody A14528 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Krishna
Verified customer
Asked: 2016-07-21
Answer
The products we sell, including anti-GPR113 antibody A14528, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-07-21
Question
Does anti-GPR113 antibody A14528 work on primate WB with left adrenal gland?
D. Zhang
Verified customer
Asked: 2016-05-27
Answer
Our lab technicians have not tested anti-GPR113 antibody A14528 on primate. You can run a BLAST between primate and the immunogen sequence of anti-GPR113 antibody A14528 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate left adrenal gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-27
