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EWSR1 antibody (M00589) performs well in IHC, WB and shows reactivity with Human, Monkey, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of EWSR1 using anti-EWSR1 antibody (M00589).
EWSR1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-EWSR1 Antibody (M00589) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
EWSR1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-EWSR1 Antibody (M00589) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Designing a Western blot for EWSR1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the EWSR1 Western Blot GuideBundle Save
Anti-EWSR1 Antibody Picoband® (monoclonal, 4B4)
EWSR1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse | Mouse | IHC, WB |
Tested Applications
EWSR1 antibody was positively detected by WB in:
K562 whole cell, Jurkat whole cell, A549 whole cell, MCF-7 whole cell, COLO-320 whole cell, SW620 whole cell, A431 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
EWSR1 antibody was positively detected by IHC in:
human placenta tissue, mouse spleen tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
Human
Tested
Recommend
Mouse
Tested
Recommend
Rat
Predicted
Predicted
Bovine
Predicted
Predicted
Dog
Predicted
Predicted
Monkey
Predicted
Predicted
Sheep
Predicted
Predicted
Product Information
Product Name
Anti-EWSR1 Antibody Picoband® (monoclonal, 4B4)
View all EWSR1 Antibodies
View all EWSR1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
4B4
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human EWSR1, different from the related mouse sequence by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
95 kDa
Calculated Molecular Weight
68.5 kDa
Background of EWSR1
This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t (11;22) (q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.
Cite This Product
Anti-EWSR1 Antibody Picoband® (monoclonal, 4B4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00589)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rat
100%
High Homology
Mouse
97%
High Homology
Cow
97%
High Homology
Dog
97%
High Homology
Monkey
97%
High Homology
Sheep
90%
High Homology
Zebrafish
45%
Low homology
Drosophila
35%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for EWSR1 antibody
Immunohistochemistry (IHC) protocol for EWSR1 antibody
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Product Promise
We promise Anti-EWSR1 Antibody Picoband® (monoclonal, 4B4) performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00589
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 25, 2026