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Product Info Summary
| SKU: | M00589 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-EWSR1 Antibody Picoband® (monoclonal, 4B4)
SKU/Catalog Number
M00589
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EWSR1 Antibody Picoband® (monoclonal, 4B4) catalog # M00589. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EWSR1 Antibody Picoband® (monoclonal, 4B4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00589)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
4B4
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human EWSR1, different from the related mouse sequence by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00589 is reactive to EWSR1 in Human, Monkey, Mouse
Observed Molecular Weight
95 kDa
Calculated molecular weight
68.5 kDa
Background of EWSR1
This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t (11;22) (q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00589 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Positive Control
WB: K562 whole cell, Jurkat whole cell, A549 whole cell, MCF-7 whole cell, COLO-320 whole cell, SW620 whole cell, A431 whole cell
IHC: human placenta tissue, mouse spleen tissue
Validation Images & Assay Conditions
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Western blot analysis of EWSR1 using anti-EWSR1 antibody (M00589).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: K562 whole cell lysates,
Lane 2: Jurkat whole cell lysates,
Lane 3: A549 whole cell lysates,
Lane 4: MCF-7 whole cell lysates,
Lane 5: COLO-320 whole cell lysates,
Lane 6: SW620 whole cell lysates,
Lane 7: A431 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-EWSR1 antigen affinity purified monoclonal antibody (Catalog # M00589) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for EWSR1 at approximately 95KD. The expected band size for EWSR1 is at 95KD.
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IHC analysis of EWSR1 using anti-EWSR1 antibody (M00589).
EWSR1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-EWSR1 Antibody (M00589) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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IHC analysis of EWSR1 using anti-EWSR1 antibody (M00589).
EWSR1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-EWSR1 Antibody (M00589) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Specific Publications For Anti-EWSR1 Antibody Picoband® (monoclonal, 4B4) (M00589)
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