Anti-EWSR1 Antibody Picoband™

EWSR1 antibody

Boster Bio Anti-EWSR1 Antibody Picoband™ catalog # PB9585. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9585
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Product Name

Anti-EWSR1 Antibody Picoband™

View all EWSR1 Antibodies

SKU/Catalog Number

PB9585

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EWSR1 Antibody Picoband™ catalog # PB9585. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EWSR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9585)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human EWSR1, different from the related mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9585 is reactive to EWSR1 in Human, Mouse, Rat

Applications

PB9585 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

95 kDa

Calculated molecular weight

68.478kDa

Background of EWSR1

This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t (11;22) (q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For EWSR1 (Source: Uniprot.org, NCBI)

Gene Name

EWSR1

Full Name

RNA-binding protein EWS

Weight

68.478kDa

Superfamily

RRM TET family

Alternative Names

bK984G1.4; Ewing sarcoma breakpoint region 1 protein; Ewing sarcoma breakpoint region 1; Ewings sarcoma EWS-Fli1 (type 1) oncogene; EWS oncogene; EWSRNA-binding protein EWS EWSR1 EWS, EWS-FLI1, bK984G1.4 EWS RNA binding protein 1 RNA-binding protein EWS|EWS RNA-binding protein variant 6|Ewing sarcoma breakpoint region 1|Ewings sarcoma EWS-Fli1 (type 1) oncogene

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EWSR1, check out the EWSR1 Infographic

EWSR1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EWSR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-EWSR1 Antibody Picoband™

Question

Has PB9585 antibody been tested on mouse samples with Western Blot, Immunocytochemistry and Immunofluorescence applications?

Verified customer

Asked: 2019-09-19

Answer

The Anti-EWSR1 Antibody Picoband PB9585 was tested on mouse samples with WB only. Our lab hasn't worked on mouse samples with Immunocytochemistry and Immunofluorescence applications due to sample limitation. It is suggested to run pilot tests for this.

Boster Scientific Support

Answered: 2019-09-20

Question

I was wanting to use your anti-EWSR1 antibody for Flow Cytometry for rat testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat testis identification?

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

You can see on the product datasheet, PB9585 anti-EWSR1 antibody has been tested for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-18

Question

We are currently using anti-EWSR1 antibody PB9585 for rat tissue, and we are satisfied with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?

B. Jones

Verified customer

Asked: 2018-10-09

Answer

The anti-EWSR1 antibody (PB9585) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-09

Question

See below the WB image, lot number and protocol we used for testis using anti-EWSR1 antibody PB9585. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-11-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-11-27

Question

Do you have a BSA free version of anti-EWSR1 antibody PB9585 available?

S. Carter

Verified customer

Asked: 2016-09-26

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-EWSR1 antibody PB9585 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-09-26

Order DetailsPrice
PB9585

100μg

$370
PB9585-10ug

10μg sample (liquid)

$99
PB9585-Biotin

100 μg Biotin conjugated

$570
PB9585-Cy3

100 μg Cy3 conjugated

$570
PB9585-Dylight488

100 μg Dylight488 conjugated

$570
PB9585-Dylight550

100 μg Dylight550 conjugated

$570
PB9585-Dylight594

100 μg Dylight594 conjugated

$570
PB9585-FITC

100 μg FITC conjugated

$570
PB9585-HRP

100 μg HRP conjugated

$570
PB9585-APC

100 μg APC conjugated

$670
PB9585-PE

100 μg PE conjugated

$670
PB9585-carrier-free

Carrier Free

$370

More conjugates/formats

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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