Click image to see more details
Product Info Summary
| SKU: | A13005-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GRASP1/GRIPAP1 Antibody Picoband®
SKU/Catalog Number
A13005-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GRASP1/GRIPAP1 Antibody Picoband® catalog # A13005-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GRASP1/GRIPAP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13005-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GRASP1/GRIPAP1 recombinant protein (Position: R42-S841).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A13005-2 is reactive to GRIPAP1 in Human, Mouse, Rat
Observed Molecular Weight
96 kDa
Calculated molecular weight
96.0 kDa
Background of GRIPAP1
GRIP1-associated protein 1 is a protein that in humans is encoded by the GRIPAP1 gene. This gene encodes a guanine nucleotide exchange factor for the Ras family of small G proteins (RasGEF). The encoded protein interacts in a complex with glutamate receptor interacting protein 1 (GRIP1) and plays a role in the regulation of AMPA receptor function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13005-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, huamn HepG2 whole cell, human RT4 whole cell, human MCF-7 whole cell, human SiHa whole cell, human T-47D whole cell, rat brain tissue, rat panceras tissue, rat testis tissue, rat PC-12 whole cell, mouse brain tissue, mouse pancreas tissue, mouse NIH/3T3 whole cell
FCM: HepG2 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of GRASP1/GRIPAP1 using anti-GRASP1/GRIPAP1 antibody (A13005-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: huamn HepG2 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: human SiHa whole cell lysates,
Lane 6: human T-47D whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: rat panceras tissue lysates,
Lane 9: rat testis tissue lysates,
Lane 10: rat PC-12 whole cell lysates,
Lane 11: mouse brain tissue lysates,
Lane 12: mouse pancreas tissue lysates,
Lane 13: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRASP1/GRIPAP1 antigen affinity purified polyclonal antibody (Catalog # A13005-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRASP1/GRIPAP1 at approximately 96 kDa. The expected band size for GRASP1/GRIPAP1 is at 96 kDa.
Click image to see more details
Flow Cytometry analysis of HepG2 cells using anti-GRASP1/GRIPAP1 antibody (A13005-2).
Overlay histogram showing HepG2 cells stained with A13005-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRASP1/GRIPAP1 Antibody (A13005-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-GRASP1/GRIPAP1 Antibody Picoband® (A13005-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GRASP1/GRIPAP1 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-GRASP1/GRIPAP1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
