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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-TLS/FUS Antibody Picoband™
Boster Bio Anti-TLS/FUS Antibody Picoband™ catalog # A00771-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TLS/FUS Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00771-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence of human TLS / FUS (DNNTIFVQGLGENVTIESVADYFKQIGIIKTNKKT).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00771-1 is reactive to FUS in Human, Mouse, Rat
A00771-1 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TLS / FUS using anti-TLS / FUS antibody (A00771-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: mouse NIH3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLS / FUS antigen affinity purified polyclonal antibody (Catalog # A00771-1) at 0.5 ug/mL overnight at 4?? then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLS / FUS at approximately 75KD. The expected band size for TLS / FUS is at 53KD.
Gene/Protein Information For FUS (Source: Uniprot.Org, NCBI)
RNA-binding protein FUS
RRM TET family
75 kDa DNA-pairing protein; ALS6; amyotrophic lateral sclerosis 6; FUS1; fused in sarcoma; fusion (involved in t(12;16) in malignant liposarcoma); fusion gene in myxoid liposarcoma; fusion, derived from t(12;16) malignant liposarcoma; fus-like protein; heterogeneous nuclear ribonucleoprotein P2; HNRNPP2; Oncogene FUS; Oncogene TLS; POMP75; RNA-binding protein FUS; TLShnRNP-P2; Translocated in liposarcoma protein FUS ALS6, ETM41, HNRNPP2, POMP75, TLS, altFUS, FUS FUS RNA binding protein RNA-binding protein FUS|75 kDa DNA-pairing protein|fus-like protein|fused in sarcoma|fusion gene in myxoid liposarcoma|heterogeneous nuclear ribonucleoprotein P2|oncogene FUS|oncogene TLS|translocated in liposarcoma protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on FUS, check out the FUS Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FUS: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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