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HSP90B1 antibody (PB9637) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Flow Cytometry analysis of THP-1 cells using anti-GRP94 antibody (PB9637).
Overlay histogram showing THP-1 cells stained with PB9637 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRP94 Antibody (PB9637,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with PB9637 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRP94 Antibody (PB9637,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-GRP94/HSP90B1 Antibody Picoband®
HSP90B1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
HSP90B1 antibody was positively detected by WB in:
human Hela whole cell, human U-87MG whole cell, human A431 whole cell, huamn CACO-2 whole cell, human THP-1 whole cell, huamn CACO-2 whole cell, huamn HepG2 whole cell, huamn HL-60 whole cell, human HEK293- WT whole cell, human HEK293-HSP90B1 KO whole cell, rat PC-12 whole cell, rat RH35 whole cell, rat C6 whole cell, mouse HEPA1-6 whole cell, mouse NIH/3T3 whole cell, mouse RAW2647 whole cell, mosue SP2/0 whole cell, mouse ANA-1 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HSP90B1 antibody was positively detected by IHC in:
human tonsil tissue, mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HSP90B1 antibody was positively detected by ICC/IF in:
A431 cell
HSP90B1 antibody was positively detected by FC in:
THP-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
1-2μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-GRP94/HSP90B1 Antibody Picoband®
View all HSP90B1 Antibodies
View all HSP90B1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GRP94 recombinant protein (Position: R43-H221). Human GRP94 shares 99.4% and 98.9% amino acid (aa) sequence identity with mouse and rat GRP94, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated Molecular Weight
92.5 kDa
Background of HSP90B1
Heat shock protein 90kDa beta member 1 (HSP90B1), known as endoplasmin, or GRP94, is a chaperone protein that in humans is encoded by the HSP90B1 gene. It is mapped to chromosome 12q23.3. This gene encodes a member of a family of adenosine triphosphate (ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation.
Cite This Product
Anti-GRP94/HSP90B1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9637)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Mouse
99%
High Homology
Rabbit
99%
High Homology
Cow
99%
High Homology
Dog
99%
High Homology
Rat
99%
High Homology
Sheep
99%
High Homology
Zebrafish
89%
Moderate Homology
Drosophila
65%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for HSP90B1 antibody
Immunohistochemistry (IHC) protocol for HSP90B1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HSP90B1 antibody
Flow Cytometry (FC) protocol for HSP90B1 antibody
Publications
Application:
Species:
Identification and validation of pyroptosis-related genes in Alzheimer’s disease based on multi-transcriptome and machine learning
Published: 2025-05-14
Journal: Frontiers in Aging Neuroscience
Application: WB
Impact Factor: 4.50
Species: Mouse
PMID: 40438507
Low miR-224-5p in exosomes confers colorectal cancer 5-FU resistance by upregulating S100A4
Published: 2025-02-06
Journal: DRUG RESISTANCE UPDATES
Application: WB
Impact Factor: 21.70
Species: Human
PMID: 39956015
Tumor-Derived Exosomal miR-29b Reduces Angiogenesis in Pancreatic Cancer by Silencing ROBO1 and SRGAP2
Published: 2022-10-14
Journal: Journal of Immunology Research
Application: WB
Impact Factor: 4.49
Species: Human
PMID: 36277474
Total flavonoids, extracted from Polygonum knotweed L, exert beneficial hepatoprotection against liver injury
Published: 2019-03-10
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: IHC
Impact Factor: 3.45
Species: Mouse
PMID: 30854697
Expression and functional characterization of NPA motif-null aquaporin-1 mutations
Published: 2009-05-26
Journal: IUBMB LIFE
Application: IF
Impact Factor: 2.36
Species: Hamster
PMID: 19472194
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Product Promise
We promise Anti-GRP94/HSP90B1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9637
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026