Anti-GRP94/HSP90B1 Antibody

gp96/HSP90B1/GRP94 antibody

Boster Bio Anti-GRP94/HSP90B1 Antibody catalog # PA1340. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: PA1340
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, ICC, WB

Product Name

Anti-GRP94/HSP90B1 Antibody

View all gp96/HSP90B1/GRP94 Antibodies

SKU/Catalog Number

PA1340

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GRP94/HSP90B1 Antibody catalog # PA1340. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GRP94/HSP90B1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1340)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human GRP94 (687-700aa INPRHPLIRDMLRR), identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1340 is reactive to HSP90B1 in Human, Mouse, Rat

Applications

PA1340 is guaranteed for IHC, ICC, WB Boster Guarantee

Background of gp96/HSP90B1/GRP94

Heat shock protein 90kDa beta member 1  (HSP90B1), known also as endoplasmin, GRP94, is a chaperone protein that in humans is encoded by the HSP90B1 gene. It is mapped to chromosome 12q23.3. This gene encodes a member of a family of adenosine triphosphate (ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For HSP90B1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P14625

Gene ID

7184

Gene Name

HSP90B1

Full Name

Endoplasmin

Weight

92.469kDa

Superfamily

heat shock protein 90 family

Alternative Names

94 kDa glucose-regulated protein; ECGP; Endoplasmin; endothelial cell (HBMEC) glycoprotein; glucose regulated protein, 94 kDa; gp96; GP96ECGP; Grp94; GRP-94; GRP94gp96 homolog; Heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; HSP90B1; TRA1endoplasmin; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen-1 (gp96) HSP90B1 ECGP, GP96, GRP94, HEL-S-125m, HEL35, TRA1 heat shock protein 90 beta family member 1 endoplasmin|94 kDa glucose-regulated protein|endothelial cell (HBMEC) glycoprotein|epididymis luminal protein 35|epididymis secretory sperm binding protein Li 125m|heat shock protein 90 kDa beta member 1|heat shock protein 90kDa beta (Grp94), member 1|heat shock protein 90kDa beta family member 1|stress-inducible tumor rejection antigen gp96|tumor rejection antigen (gp96) 1|tumor rejection antigen 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on HSP90B1, check out the HSP90B1 Infographic

HSP90B1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for HSP90B1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1340 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ibutilide protects against cardiomyocytes injury via inhibiting endoplasmic reticulum and mitochondrial stress pathways

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1 Reviews For Anti-GRP94/HSP90B1 Antibody

0

"Reliable and robust"

Excellent

Rat il 6 elisa kit

Source: Biocompare.com

"Reliable and robust"

--R.P., BIOLOGY, PENN STATE UNIVERSITY, TECHNICIAN

Applications Western Blot
Sample Cell lysate
Detection Enhanced chemiluminescence

"This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation."

Verified customer

Submitted

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7 Customer Q&As for Anti-GRP94/HSP90B1 Antibody

Question

Can you help my question with product PA1340, anti-GRP94/HSP90B1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1340 anti-GRP94/HSP90B1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-31

Question

I see that the anti-GRP94/HSP90B1 antibody PA1340 works with IHC, what is the protocol used to produce the result images on the product page?

Z. Baker

Verified customer

Asked: 2019-10-22

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-22

Question

Does anti-GRP94/HSP90B1 antibody PA1340 work for IHC with melanoma?

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

According to the expression profile of melanoma, HSP90B1 is highly expressed in melanoma. So, it is likely that anti-GRP94/HSP90B1 antibody PA1340 will work for IHC with melanoma.

Boster Scientific Support

Answered: 2019-06-25

Question

Would anti-GRP94/HSP90B1 antibody PA1340 work on feline WB with skin?

Verified Customer

Verified customer

Asked: 2019-06-14

Answer

Our lab technicians have not tested anti-GRP94/HSP90B1 antibody PA1340 on feline. You can run a BLAST between feline and the immunogen sequence of anti-GRP94/HSP90B1 antibody PA1340 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline skin in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-14

Question

Is a blocking peptide available for product anti-GRP94/HSP90B1 antibody (PA1340)?

J. Patel

Verified customer

Asked: 2018-04-09

Answer

We do provide the blocking peptide for product anti-GRP94/HSP90B1 antibody (PA1340). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-04-09

Question

Is there a BSA free version of anti-GRP94/HSP90B1 antibody PA1340 available?

Verified Customer

Verified customer

Asked: 2017-10-03

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-GRP94/HSP90B1 antibody PA1340 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-10-03

Question

Here is the WB image, lot number and protocol we used for melanoma using anti-GRP94/HSP90B1 antibody PA1340. Please let me know if you require anything else.

R. Wu

Verified customer

Asked: 2014-12-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-12-18

Size

Total: $315

SKU:PA1340

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
PA1340
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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