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Product Info Summary
| SKU: | M05856 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Monkey |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-H6PD Mouse Monoclonal Antibody [Clone ID: OTI2A7]
SKU/Catalog Number
M05856
Size
100 µl
Description
Boster Bio Anti-H6PD mouse monoclonal antibody, clone OTI2A7 (formerly 2A7). Catalog# M05856. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-H6PD Mouse Monoclonal Antibody [Clone ID: OTI2A7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05856)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2A7
Isotype
IgG1
Immunogen
Full length human recombinant protein of human H6PD (NP_004276) produced in HEK293T cell.
Reactive Species
M05856 is reactive to H6PD in Human, Monkey
Calculated molecular weight
88.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05856 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:250~500
IHC 1:50
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY H6PD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-H6PD.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-H6PD monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-H6PD monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-H6PD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-H6PD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-H6PD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-H6PD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either H6PD (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-H6PD antibody (M05856)
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Flow cytometric Analysis of Jurkat cells
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Anti-H6PD mouse monoclonal antibody (M05856) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY H6PD.
Specific Publications For Anti-H6PD Mouse Monoclonal Antibody [Clone ID: OTI2A7] (M05856)
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