Human Thioredoxin ELISA Kit PicoKine™

Human Thioredoxin ELISA Kit PicoKine™ (96 Tests). Quantitate Human TXN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1254
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human Thioredoxin ELISA Kit PicoKine™

See all Thioredoxin-1 products

SKU/Catalog Number

EK1254

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Thioredoxin ELISA Kit PicoKine™ (96 Tests). Quantitate Human TXN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Thioredoxin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1254)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V2-V105

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1254 is reactive to TXN in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1254 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1254-CAP Anti-Human TXN Pre-coated 96-well strip microplate 1
EK1254-ST Human TXN Standard 2 vials, 10 ng/tube
EK1254-DA Human TXN Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0160.1120.2260.3700.6431.1141.5661.925

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Thioredoxin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3001212458532913014269
Standard deviation18.683.62183.420.7289.76204.91
CV (%)6.2%6.9%4%6.9%6.9%4.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Thioredoxin-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 13002842852922906.412.2%
Sample 21212105611481119113356.094.9%
Sample 345855257493151114971250.995%
*number of samples for each test n=16.

Gene/Protein Information For TXN (Source: Uniprot.Org, NCBI)

Uniprot ID

P10599

Gene ID

7295

Gene Name

TXN

Full Name

Thioredoxin

Weight

11.737kDa

Superfamily

thioredoxin family

Alternative Names

Thioredoxin1; Thioredoxin-1; Trx1; TXN; TXN1 TXN TRDX, TRX, TRX1, Trx80 thioredoxin thioredoxin|ADF|ATL-derived factor|SASP|TXN delta 3|surface-associated sulphydryl protein|testicular tissue protein Li 199|thioredoxin delta 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TXN, check out the TXN Infographic

TXN infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TXN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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8 Customer Q&As for Human Thioredoxin ELISA Kit PicoKine™

Question

Q: how to analyze ELISA data? I have obtained Thioredoxin-1 level in serum.

Verified Customer

Verified customer

Asked: 2020-08-28

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-08-28

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Thioredoxin-1 using the EK1254 Human Thioredoxin-1 Picokine® ELISA Kit?

T. Thomas

Verified customer

Asked: 2019-07-13

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2019-07-13

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Thioredoxin-1 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-02-01

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Thioredoxin-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-02-01

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

W. Perez

Verified customer

Asked: 2018-11-10

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-11-10

Question

Q: What is the optimal O.D. value for Thioredoxin-1 ELISA kit? I used your Thioredoxin-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Thioredoxin-1 even though the O.D. values are not very high?

J. Jackson

Verified customer

Asked: 2018-06-28

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Thioredoxin-1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Thioredoxin-1 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-06-28

Question

Q: can I use citrate plasma as samples in Human Thioredoxin-1 Picokine® ELISA Kit (Catalog # EK1254)?

Verified Customer

Verified customer

Asked: 2018-02-04

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Thioredoxin-1 causing disruption of its protein structure. Thioredoxin-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Thioredoxin-1 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-02-04

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2017-04-23

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-04-23

Question

Q: if the enzyme conjugated Thioredoxin-1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Thioredoxin-1 antigen?

Verified Customer

Verified customer

Asked: 2014-07-13

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2014-07-13

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