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Product Info Summary
| SKU: | PA1552 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-hCG receptor/LHCGR Antibody Picoband®
SKU/Catalog Number
PA1552
BA3590 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-hCG receptor/LHCGR Antibody catalog # PA1552. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-hCG receptor/LHCGR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1552)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human hCG receptor, different from the relative rat sequence by two amino acids, mouse sequence by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1552 is reactive to LHCGR in Human, Rat
Observed Molecular Weight
78 kDa
Calculated molecular weight
78.6 kDa
Background of LHCGR
The luteinizing hormone/choriogonadotropin receptor is a member of a subfamily of G protein-coupled receptors (GPCR) characterized by the presence of a large N-terminal extracellular domain containing several leucine-rich repeats (LRR). This glycoprotein hormone receptor family has been named the LRR-containing GPCR (LGR) family. The LHCGR gene contains 11 exons and spans approximately 80 kb and it is mapped to chromosome 2p21. In the ovary, theca, stromal, late-stage (luteinizing) granulosa, and luteal cells contain LHCGR. In the testes, Leydig cells contain LHCRG.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1552 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: MCF-7 Whole Cell
IHC: Rat Ovary Tissue
Validation Images & Assay Conditions
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Anti-hCG receptor antibody, PA1552, IHC(P)
IHC(P): Rat Ovary Tissue
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Anti-hCG receptor antibody, PA1552, Western blotting
All lanes: Anti hCG receptor (PA1552) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 78KD
Observed bind size: 78KD
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IHC analysis of LHCGR using anti-LHCGR antibody (PA1552).
LHCGR was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHCGR Antibody (PA1552) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Distribution of aldosterone synthase (CYP11B2) and LHCGR in the patient’s adrenal. (A) , Distribution of CYP11B2 immunoreactivity in the tumor region (T) of the adrenal tissue. (B-E) , Distribution of CYP11B2 (B, D) and LHCGR (C, E) immunoreactivities in consecutive sections of the APA tissue at low (B, C) and high (D, E) magnifications. Similar distribution of CYP11B2 and LHCGR immunoreactivities were observed in some areas (arrows) in (B, C) . PT indicates peritumoral tissue; and V, vein.
Index in PubMed under a CC BY license. PMID: 36926028
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The effects of treatment on ovarian FSHR, LHR, ER, and PR expression level. ( A ) The FSHR, LHR, ER, and PR were detected by immunohistochemistry, red arrows represent the expression of receptors. ( B ) The positive rate of FSHR, LHR, ER, and PR in four groups. *Represents MPA + hMG group vs control group, MPA group or hMG group. *P < 0.05.
Index in PubMed under a CC BY license. PMID: 29535409
Specific Publications For Anti-hCG receptor/LHCGR Antibody Picoband® (PA1552)
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Customer Q&As
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2 Customer Q&As for Anti-hCG receptor/LHCGR Antibody Picoband®
Question
What are your suggestions to reduce the background for my western blot? Keyword: troubleshooting, protocol support
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
Our technicians provide these suggestions to reduce the background: 1.Block the membrane 2 hours at room temperature with 5% no-fat milk in TBS-T; 2.Dilute second antibody with 5% no-fat milk in TBS-T; and 3.Extend the wash time to 10 minutes each time.
Boster Scientific Support
Answered: 2019-10-21
Question
We are currently using anti-hCG receptor/LHCGR antibody PA1552 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
The anti-hCG receptor/LHCGR antibody (PA1552) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-20
