Anti-HDAC-2 (C-terminus) Antibody

Product Name

Anti-HDAC-2 (C-terminus) Antibody

View all HDAC2 Antibodies

SKU/Catalog Number

A00325

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-HDAC-2 (C-terminus) Antibody (Catalog # A00325). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Primate.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening. (Ship on dry ice.)

Cite This Product

Anti-HDAC-2 (C-terminus) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00325)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol

Clonality

Polyclonal

Isotype

IgG

Immunogen

HDAC2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus region of human HDAC2.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00325 is reactive to HDAC2 in Human, Mouse, Rat, Primate

Observed Molecular Weight

42 kDa

Calculated molecular weight

55.4 kDa

Background of HDAC2

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDAC2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human. Anti-HDAC2 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Chromatin Research, Epigenetics, Histone Deacetylases, and Stem Cell Markers research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00325 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:60,000
IHC: 1:100-1:500
IF Microscopy: 1:100-1:500
WB: 1 µg/ml
Anti-HDAC2 antibody is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~59 kDa corresponding to the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information HDAC2

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