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Product Info Summary
| SKU: | PB9629 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HDAC7 Antibody Picoband®
SKU/Catalog Number
PB9629
PB0673 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HDAC7 Antibody Picoband® catalog # PB9629. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HDAC7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9629)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HDAC7, different from the related mouse and rat sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9629 is reactive to HDAC7 in Human, Rat
Observed Molecular Weight
103 kDa
Calculated molecular weight
102.9 kDa
Background of HDAC7
Histone deacetylase 7 is an enzyme that in humans is encoded by the HDAC7 gene. It is mapped to 12q13.1. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation / deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9629 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Rat Spleen Tissue, Rat Thymus Tissue, Human Placenta Tissue, HELA Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of HDAC7 using anti-HDAC7 antibody (PB9629).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Spleen Tissue Lysate at 50ug,
Lane 2: Rat Thymus Tissue Lysate at 50ug,
Lane 3: Human Placenta Tissue Lysate at 50ug,
Lane 4: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC7 antigen affinity purified polyclonal antibody (Catalog # PB9629) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC7 at approximately 103 kDa. The expected band size for HDAC7 is at 103 kDa.
Specific Publications For Anti-HDAC7 Antibody Picoband® (PB9629)
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Customer Q&As
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5 Customer Q&As for Anti-HDAC7 Antibody Picoband®
Question
Is a blocking peptide available for product anti-HDAC7 antibody (PB9629)?
R. Taylor
Verified customer
Asked: 2019-11-01
Answer
We do provide the blocking peptide for product anti-HDAC7 antibody (PB9629). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-11-01
Question
Does anti-HDAC7 antibody PB9629 work for WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
According to the expression profile of leukemic t-cell, HDAC7 is highly expressed in leukemic t-cell. So, it is likely that anti-HDAC7 antibody PB9629 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2019-10-17
Question
We are currently using anti-HDAC7 antibody PB9629 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-10
Answer
The anti-HDAC7 antibody (PB9629) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-10
Question
I was wanting to use to test anti-HDAC7 antibody PB9629 on rat leukemic t-cell for research purposes, then I may be interested in using anti-HDAC7 antibody PB9629 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-22
Answer
The products we sell, including anti-HDAC7 antibody PB9629, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-22
Question
I see that the anti-HDAC7 antibody PB9629 works with WB, what is the protocol used to produce the result images on the product page?
O. Miller
Verified customer
Asked: 2015-11-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-11-17
