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Product Info Summary
| SKU: | M00010-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-HER2 ERBB2 Monoclonal Antibody
SKU/Catalog Number
M00010-3
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HER2 ERBB2 Monoclonal Antibody catalog # M00010-3. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HER2 ERBB2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00010-3)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
11H9
Isotype
IgG
Immunogen
Synthetic Peptide of HER2
Reactive Species
M00010-3 is reactive to ERBB2 in Human, Mouse, Rat
Calculated molecular weight
137.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00010-3 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000-4000
IHC 1:200
IF 1:200
Validation Images & Assay Conditions
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Western blot analysis of lysates from 1) Hela, 2) A431, 3) MCF-7 cells, (Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red） Actin β Polyclonal Antibody was diluted at 1:5000 as loading control, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.
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Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, HER2 Monoclonal Antibody (11H9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, HER2 Monoclonal Antibody (11H9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunofluorescence analysis of Hela cell. 1, E2F-1 Polyclonal Antibody (red) was diluted at 1:200 (4° overnight). HER2 Monoclonal Antibody (11H9) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
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Immunofluorescence analysis of Rat-spleen tissue. 1, HER2 Monoclonal Antibody (11H9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Specific Publications For Anti-HER2 ERBB2 Monoclonal Antibody (M00010-3)
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