Anti-HERC5 Antibody Picoband™

HERC5 antibody

Boster Bio Anti-HERC5 Antibody Picoband™ catalog # A05285-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A05285-2
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, IF, ICC, WB

Product Name

Anti-HERC5 Antibody Picoband™

View all HERC5 Antibodies

SKU/Catalog Number

A05285-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HERC5 Antibody Picoband™ catalog # A05285-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HERC5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05285-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human HERC5 recombinant protein (Position: F793-G1024).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05285-2 is reactive to HERC5 in Human

Applications

A05285-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

117 kDa

Calculated molecular weight

116.852kDa

Background of HERC5

Probable E3 ubiquitin-protein ligase HERC5 is an enzyme that in humans is encoded by the HERC5 gene. It is mapped to 4q22.1. This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For HERC5 (Source: Uniprot.org, NCBI)

Gene Name

HERC5

Full Name

E3 ISG15--protein ligase HERC5

Weight

116.852kDa

Alternative Names

CEB1E3 ISG15--protein ligase HERC5; CEBP1; cyclin-E binding protein 1; Cyclin-E-binding protein 1; EC 6.3.2; EC 6.3.2.-; HECT domain and RCC1-like domain-containing protein 5; hect domain and RLD 5; HECT E3 ubiquitin ligase; probable E3 ubiquitin-protein ligase HERC5 HERC5 CEB1, CEBP1 HECT and RLD domain containing E3 ubiquitin protein ligase 5 E3 ISG15--protein ligase HERC5|HECT domain and RCC1-like domain-containing protein 5|cyclin-E-binding protein 1|hect domain and RLD 5|probable E3 ubiquitin-protein ligase HERC5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HERC5, check out the HERC5 Infographic

HERC5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HERC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-HERC5 Antibody Picoband™

Question

Here is the WB image, lot number and protocol we used for embryonic kidney using anti-HERC5 antibody A05285-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-24

Question

We are currently using anti-HERC5 antibody A05285-2 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?

Z. Yang

Verified customer

Asked: 2019-12-23

Answer

The anti-HERC5 antibody (A05285-2) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-23

Question

Is this A05285-2 anti-HERC5 antibody reactive to the isotypes of HERC5?

Verified Customer

Verified customer

Asked: 2018-08-23

Answer

The immunogen of A05285-2 anti-HERC5 antibody is E.coli-derived human HERC5 recombinant protein (Position: F793-G1024). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-08-23

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-HERC5 antibody A05285-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-03-05

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-03-05

Question

Will A05285-2 anti-HERC5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-01-22

Answer

It shows on the product datasheet, A05285-2 anti-HERC5 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-01-22

Order DetailsPrice
A05285-2

100μg

$370
A05285-2-10ug

10μg sample (liquid)

$99
A05285-2-Biotin

100 μg Biotin conjugated

$570
A05285-2-Cy3

100 μg Cy3 conjugated

$570
A05285-2-Dylight488

100 μg Dylight488 conjugated

$570
A05285-2-Dylight550

100 μg Dylight550 conjugated

$570
A05285-2-Dylight594

100 μg Dylight594 conjugated

$570
A05285-2-FITC

100 μg FITC conjugated

$570
A05285-2-HRP

100 μg HRP conjugated

$570
A05285-2-APC

100 μg APC conjugated

$670
A05285-2-PE

100 μg PE conjugated

$670
A05285-2-carrier-free

Carrier Free

$370

More conjugates/formats

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A05285-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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