Anti-HERC5 Antibody Picoband™

Boster Bio Anti-HERC5 Antibody Picoband™ catalog # A05285-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A05285-2
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, IF, ICC, WB

Product Name

Anti-HERC5 Antibody Picoband™

See all HERC5 products

SKU/Catalog Number

A05285-2

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HERC5 Antibody Picoband™ catalog # A05285-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HERC5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05285-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human HERC5 recombinant protein (Position: F793-G1024).

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A05285-2 is reactive to HERC5 in Human

Applications

A05285-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For HERC5 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9UII4

Gene ID

51191

Gene Name

HERC5

Full Name

E3 ISG15--protein ligase HERC5

Weight

116.852kDa

Alternative Names

CEB1E3 ISG15--protein ligase HERC5; CEBP1; cyclin-E binding protein 1; Cyclin-E-binding protein 1; EC 6.3.2; EC 6.3.2.-; HECT domain and RCC1-like domain-containing protein 5; hect domain and RLD 5; HECT E3 ubiquitin ligase; probable E3 ubiquitin-protein ligase HERC5 HERC5 CEB1, CEBP1 HECT and RLD domain containing E3 ubiquitin protein ligase 5 E3 ISG15--protein ligase HERC5|HECT domain and RCC1-like domain-containing protein 5|cyclin-E-binding protein 1|hect domain and RLD 5|probable E3 ubiquitin-protein ligase HERC5

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on HERC5, check out the HERC5 Infographic

HERC5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for HERC5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Anti-HERC5 Antibody Picoband™

Question

Here is the WB image, lot number and protocol we used for embryonic kidney using anti-HERC5 antibody A05285-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-24

Question

We are currently using anti-HERC5 antibody A05285-2 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?

Z. Yang

Verified customer

Asked: 2019-12-23

Answer

The anti-HERC5 antibody (A05285-2) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-23

Question

Is this A05285-2 anti-HERC5 antibody reactive to the isotypes of HERC5?

Verified Customer

Verified customer

Asked: 2018-08-23

Answer

The immunogen of A05285-2 anti-HERC5 antibody is E.coli-derived human HERC5 recombinant protein (Position: F793-G1024). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-08-23

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-HERC5 antibody A05285-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-03-05

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-03-05

Question

Will A05285-2 anti-HERC5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-01-22

Answer

It shows on the product datasheet, A05285-2 anti-HERC5 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-01-22

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A05285-2
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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