Click image to see more details
Product Info Summary
| SKU: | A05285-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-HERC5 Antibody Picoband®
SKU/Catalog Number
A05285-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HERC5 Antibody Picoband® catalog # A05285-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HERC5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05285-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HERC5 recombinant protein (Position: F793-G1024).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05285-2 is reactive to HERC5 in Human
Observed Molecular Weight
117 kDa
Calculated molecular weight
116.9 kDa
Background of HERC5
Probable E3 ubiquitin-protein ligase HERC5 is an enzyme that in humans is encoded by the HERC5 gene. It is mapped to 4q22.1. This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05285-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human 293T whole cell, human A549 whole cell, human PC-3 whole cell
ICC/IF: U2OS cell
Validation Images & Assay Conditions
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Western blot analysis of HERC5 using anti-HERC5 antibody (A05285-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HERC5 antigen affinity purified polyclonal antibody (Catalog # A05285-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HERC5 at approximately 117 kDa. The expected band size for HERC5 is at 117 kDa.
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IF analysis of HERC5 using anti-HERC5 antibody (A05285-2).
HERC5 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-HERC5 Antibody (A05285-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-HERC5 Antibody Picoband® (A05285-2)
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5 Customer Q&As for Anti-HERC5 Antibody Picoband®
Question
Here is the WB image, lot number and protocol we used for embryonic kidney using anti-HERC5 antibody A05285-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-24
Question
We are currently using anti-HERC5 antibody A05285-2 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Z. Yang
Verified customer
Asked: 2019-12-23
Answer
The anti-HERC5 antibody (A05285-2) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-23
Question
Is this A05285-2 anti-HERC5 antibody reactive to the isotypes of HERC5?
Verified Customer
Verified customer
Asked: 2018-08-23
Answer
The immunogen of A05285-2 anti-HERC5 antibody is E.coli-derived human HERC5 recombinant protein (Position: F793-G1024). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-23
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-HERC5 antibody A05285-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-03-05
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-05
Question
Will A05285-2 anti-HERC5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-22
Answer
It shows on the product datasheet, A05285-2 anti-HERC5 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-22
